Isolation of Phenolate Type Siderophore from Pseudomonas sp. PY002

Phenolate type siderophore was produced in Pseudomonas sp. PY002 (P. sp. PY002) which cultured in M9 minimal medium supplemented with 0 to 500 uM of dipyridyl. Pyochelin, a kind of siderophore, was detected as a single broad absorption band (280 nm) at pH 12.0, which is a characteristic of phenolate type siderophore. The 280 nm absorption spectrum of pyochelin was changed to 310 nm at pH 1.5. The pyochelin produced was a structurally unique phenolate siderophore, designated 2-[2-(o-hydroxyphenyl)-2-thiazolin-4-yl]-3-methyl-4-thiazolidine car- boxylic acid on the analysis of infrared radiation and 'H and ""C nuclear magnetic resonance spectroscopy. Additionally, purified pyochelin increased the cell growth rate, like as growth fac- tor. All these results suggest that phenolate type siderophore play an important role in cell growth of P. sp. PY002.

Biological characteristics and serovars of vibrio vulnificus

No abstract available.

Lumbar Epidural Venography

The clinical value of lumbar epidural venography(L.E.V.) has been stressed in diagnosing lumbar disc since Batson's desciption of vertebral venous system in 1940. We have tried this procedure by the selective catheterization of ascending lumbar and, or internal iliac vein to 16 patients with disc symptoms. In 6 patients with abnormal myelography, 4 revealed abnormal L.E.V. and in 2, the above procedure was failed. In 6 patients with disc-like symptoms but normal myelography, 2 were abnormal L.E.V., 2 normal and 2 failed. 3 with disc-like symptoms but equivocal myelography, abnormal L.E.V. was definitely shown in 2 patients and failed. Operation was done in 7 patients(6, abnormal myelography and one, only abnormal L.E.V.).

Intractable Pain Relief by the Intrathecal Neurolytic Agents

Chemical rhizotomy with the intrathecal alcohol injection was firstly attempted by Dogliotti in 1931, and with phenol in 1955 by Maher. The intrathecal neurolytic injection was a very simple, effective and nonsurgical procedure without any great danger. Good indications for the procedure are the bilateral pelvic and lower limb pain syndrome, well circumscribed pain in limited area and in poor general condition. We have reported two cases of intractable pain of limited area, successfully relieved by chemical rhizotomy, and discussed the importance of the procedure and presented the review of literatures.

Two Cases of Cerebral Arteriovenous Malformation

We experienced 2 cases of cerebral arteriovenous malformation(AVM) with tital extirpation recently. One patient, 23 year old man had chief complaints of sudden headache and loss of consciousness of 3 days duration. On admission, the patient was in a drowsy mental state and had mild papilledema on both sides. Other neurological and physical examinations were normal. Right carotid angiogram revealed AVM which is fed from the right middle cerebral artery and is drained into the right transverse sinus. We found intracerebral hematoma(100 cc) on operative field. The other patient, 30 year old man had chief complaints of Jackson's type seizure several times on the left hand during a five month duration and also had a severe headache intermittently for three months. Left carotid angiogram revealed AVM which is fed from the left anterior cerebral artery and left middle meningeal artery, and is drained into the superior sagittal sinus. We did not find intracerebral hematoma but found an old hemorrhage pouch. The 2 cases of AVM lesions were not found on the postoperative carotid angiogram.

A Case of Axis Body Fracture Complicating Brown-Sequard Syndrome

Fracture of odontoid process is relatively common, but fracture of axis body without fracture of odontoid precess is known to be exceptionally. Recently, we have experienced a case of axis body fracture complicating typical Brown-Sequard syndrome below the level of C4, and we present the case with the review of literature.