Bacterial translocation from the gut complicating hemorrhagic shock

To determine whether hemorrhagic shock would promote bacterial translocation from the gut to visceral organs, rabbits were subjected to a model of hemorrhagic shock [mean pressure, 30 mm/Hg]. Twenty- four hours after being subjected to sham-shock, or 90 minutes of shock, rabbits were sacrificed and their organs quantitatively cultured for translocating bacteria. There was a direct relationship between the duration of hemorrhagic shock and the 24-hours mortality rate. Bacteria did not translocate from the gut in the sham-shock rabbits, but translocated to the mesenteric lymph nodes, livers and spleens of the rabbits subjected to hemorrhagic shock. Rabbits subjected to 90 minutes of shock exhibited a greater degree of bacterial translocation than rabbits receiving 30 or 60 minutes of shock; whereas the incidences of translocation were significantly [P < 0.01 and P < 0.001] increased with the increase of duration of hemorrhagic shock. The most common translocating bacteria were Escherichia coli and Enterococcus. Hemorrhagic shock produced subepithelial edema and focal areas of necrosis in the ileum, vacuolization of the renal epithelial cells with cellular debris within the renal tubules, and hepatic centrilobular venous congestion. Thus hemorrhagic shock followed by reinfusion of shed blood disrupts the gut barrier and allows indigenous bacteria normally contained within the gut to cause systemic infections

Gynocheck endometrial tissue sampler: an evaluation of an out-patient procedure for endometrial investigation

The Gynocheck endometrial tissue sampler was used to obtain endometrial tissue from 80 consecutive patients presenting with infertility premenopausal bleeding and postmenopausal bleeding, without anesthesia. Then the classic cervical dilation and endometrial curetting [D. and C.] was done under general anesthesia. Patient's acceptances, thime taken adequacy of sample, and reliability for histopathological diagnosis were evaluated. Different pathological results were obtained and were correlated 100% with the samples taken by D. and C. The failure rate and inadequacy of sample was very limited to only one case [1.25%]. This method was found to be raid, easy, accepted by the patients and reliable. Also it is superior to the cytology samplers being more reliable

role of orcien alcian blue in the discrimination between dysplasia and reactive hyperplasia in ulcerative colitis

30 cases of different stages of ulcerative colitis were stained by Hx and E and orcien alcian blue to study the mucin changes in cases of dysplasia and reactive hyperplasia. 6 out of the 8 villous patterns encountered, although no morphologic evidence of dysplasia is encountered, yet the histochemical results were markedly deviated from normal. All 8 cases with adenomatous patterns proved to reveal mucin changes in the form of sialomucins predominance. The lining mucosa away in cases of ulcerative colitis revealed a predominance of sialomucins and it is called transitional mucosa which must be regarded with great caution