Progress in surgical diagnosis and treatment of pulmonary small nodules / 第二军医大学学报

Pulmonary small nodules are common diseases of the lung, and are the initial stage of lung cancer progression. This review describes the definition of pulmonary small nodules, the identification methods of benign and malignant pulmonary small nodules, and the common diagnostic methods for pulmonary small nodules. In addition, we compared the follow-up strategies of pulmonary small nodules in common guidelines at home and abroad. We also explained the principles of minimally invasive surgery, precise treatment and accelerated rehabilitation of pulmonary small nodules, providing directions for the clinical diagnosis and treatment of pulmonary small nodules.

Uniportal video-assisted thoracoscopic anatomic pulmonary segmentectomy for small pulmonary lesions: A clinical analysis of 395 cases / 第二军医大学学报

ObjectiveTo explore the feasibility and safety of uniportal video-assisted thoracoscopic anatomic pulmonary segmentectomy in the treatment of benign and malignant small lung lesions. MethodsA retrospective analysis was conducted on the clinical data of 395 patients with small pulmonary lesions undergoing uniportal videoassisted thoracoscopic anatomic pulmonary segmentectomy by a single surgery group between Aug. 2013 and May 2017 in Shanghai Pulmonary Hospital of Tongji University. Among them, there were 139 males and 256 females, with a mean age of (56.25±10.53) years, and 193 left lung lesions and 202 right lung lesions. The double-lumen trachea cannula was performed under general anesthesia. Thoracoscopy and surgical instruments were inserted through a 4 cm single-hole incision in the 4th or 5th intercostal of anterior line axillary, and anatomical segmentectomy was performed. The perioperative data, postoperative pathology, intraoperative open thoracotomy transit, postoperative complications and long-term follow-up results were statistically analyzed. ResultsOf the 395 patients, 7 patients were converted to lobectomy, 3 to thoracotomy, 1 to basal pulmonary segmentectomy from posterior basal pulmonary segment, and all the other patients were successfully completed. There were no significant differences in the operation time, intraoperative blood loss, lesion diameter, number of lymph node samples, thoracic drainage at 24 h after operation, postoperative thoracic drainage tube insertion time or postoperative hospital stay between left and right lung lesions (all P>0.05). Postoperative pathology showed malignant lesions in 310 cases and benign lesions in 85 cases. Postoperative complications occurred in 11 cases (6 cases of left lung and 5 cases of right lung), including 2 cases of hemothorax, 6 cases of delayed leakage, and 3 cases of arrhythmia. There were no operative death. The average follow-up time was (40.06±2.86) months, ranging from 9 to 54 months. There were no long-term complications, no abnormal cardiopulmonary function and no recurrence or metastasis in patients with malignant tumors. Conclusion The uniportal video-assisted thoracoscopic anatomic pulmonary segmentectomy is safe and feasible, and can resect lesions accurately and minimally invasively. And thus it is worthy of clinical application.

Effect of platelet-rich plasma on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of platelet-rich plasma (PRP) on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.</p><p><b>METHODS</b>Human adipose-derived stem cells were obtained by enzymatic digestion and PRP was prepared by dual centrifugal method. The ADSCS were interfused with 5%, 10%, and 20% PRP in conditioned culture media, using the untreated cells as the control group. The morphology of the cells were observed and their proliferative ability was detected using XTT colorimetric assay. The adipogenic differentiation ability of the cells was evaluated using oil Red O staining.</p><p><b>RESULTS</b>The ADSCS treated with PRP showed better morphology with higher density than the control cells. XTT colorimetric assay demonstrated obviously stronger proliferative activity of PRP-treated cells than the control group (P<0.01). Interfusion with PRP caused a significant increase in adipogenic differentiation of the cells as compared to the control cells (P<0.01).</p><p><b>CONCLUSION</b>PRP treatment produces obvious effects on the proliferation and adipogenic differentiation of human adipose-derived stem cells in vitro.</p>

Study of adipose tissue engineering with human adipose-derived stem cells and collagen type I scaffold / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the possibility of building tissue-engineered adipose tissue and looking for a new approach for the repair of soft tissue defects.</p><p><b>METHODS</b>The cells using enzymatic digestion from human liposuction part of the lipid extract were used as adipose tissue-derived cells and labeled with DiI fluorescent marker, the induced group using I collagen scaffold material as a carrier, the induced cell were planted into left back subcutaneously in nude mice at 1 x 10(7)/ml cell density, in the uninduced group cells were not induced by any, in the same cell density and type I collagen scaffold composite inoculated in nude right mouse back skin, the blank control group I collagen scaffold gaps in nude mice inoculated subcutaneously center of the neck, each of the six mice; Remove implants after 12 weeks and judge the adipogenic capacity through general and fluorescence microscopy, wet - determination, histological detection and oil red O staining qualitative.</p><p><b>RESULTS</b>The primary source of fat cultured stem cells, similar to the fibroblast morphology, and has a strong proliferative capacity. In the role of adipose differentiation medium, it can be the mature fat cells in which cytoplasmic lipid droplets gather, oil red O staining was positive. In the induced group, newborn tissue were found in the experimental groups of nude mice and its average weight is about 0.020 g. Conventional pathological slices and oil red O staining confirmed it is mature adipose tissue, the fluorescence staining positive confirm them are exogenous. Uninduced group newborn tissue are found in the experimental groups of nude mice and its average weight is about 0.014 g. Conventional pathological slices and oil red O staining confirmed it include some mature adipose tissue, the fluorescence staining positive confirm them are exogenous. Two groups of the new wet weight with have statistical significance (P < 0.01); gaps in the control group no new organization formed.</p><p><b>CONCLUSIONS</b>The cells using enzymatic digestion from human liposuction part of the lipid extract are adipose tissue-derived cells. The cells can be as seed cells and with solid scaffold of collagen type I it can become fat tissue in vivo successfully.</p>

Cellular compatibility of type collagen I scaffold and human adipose-derived stem cells / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the cellular compatibility of type I collagen scaffold and human adipose-derived stem cells (ADSC(S)) in order to explore appropriate scaffold materials for adipose tissue engineering.</p><p><b>METHODS</b>The morphology and function of the ADSC(S) were observed by inverted phase contrast microscope, scanning electron microscope and XTT assay when cocultured type I collagen scaffold with ADSC(S) in vitro. Cells adhesive rates were also calculated.</p><p><b>RESULT</b>ADSC(S) were able to attach, grow and proliferate well on the scaffolds.</p><p><b>CONCLUSION</b>collagen I scaffold exhibits excellent cellular compatibility and can be used as a vehicle for adipose tissue engineering.</p>

Effects of 5F from Pteris semipinnate L on growth of human pathological scars in nude mice / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of 5F from Pteris semipinnate L on the growth of human pathological scar in nude mice.</p><p><b>METHODS</b>5F from Pteris semipinnate L was administered at different doses in nude mouse models bearing human pathological scars. The morphology, histology, tumor growth factor-beta1 and type I collagen content of the scar tissues were examined after the administration.</p><p><b>RESULTS</b>Administration of 5F significantly reduced the volume of the implanted pathological scars in the nude mouse models, and histologically, the scar tissue exhibited a transition to the normal scar architecture with decreased TGF-beta1 and type I collagen content.</p><p><b>CONCLUSION</b>5F could effectively inhibit the growth of pathological scars in nude mice.</p>