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1.
Chinese Journal of Experimental and Clinical Virology ; (6): 64-66, 2007.
Article Dans Chinois | WPRIM | ID: wpr-305493

Résumé

<p><b>OBJECTIVE</b>To observe the therapeutic effect of autologous cytokine-induced killer cells (CIK) on HBV DNA positive patients with liver cirrhosis.</p><p><b>METHODS</b>HBV DNA positive 33 patients with cirrhosis were treated with CIK. Before and after cultured in vitro and post-treatment, CD3+, CD3+CD4+, CD3+CD8+, CD3+CD56+ cells, mDC and pDC were detected by flow cytometry. The indexes of virus and liver function were compared between pre- and post-treatment.</p><p><b>RESULTS</b>CD3+, CD3+CD8+ cells and CD3+CD56+ cells were higher after cultured in vitro and after transfused back than those before culture (91.5 +/- 10.3, 74.4 +/- 9.9 vs. 67.9 +/- 12.8; 60.9 +/- 15.5, 37.3 +/- 15.1 vs. 27.9 +/- 10.9; 18.4 +/- 11.7, 14.5 +/- 7.5 vs. 10.6 +/- 7.1). The percentages of mDC and pDC also increased after-treatment vs. pre-treatment (0.54 +/- 0.18 vs. 0.70 +/- 0.29; 0.26 +/- 0.13 vs. 0.41 +/- 0.25). HBV DNA became undetectable in 12 patients and decrease exceeded 100 times in 4 patients after treatment. HBeAg became undetectable in 10 of 14 patients who were HBeAg positive pretreatment patients, among them 2 patients had HBeAb sero conversion. The liver function was improved after treatment. All patients tolerated the treatment.</p><p><b>CONCLUSION</b>CIK treatment can increase immune effector cells and has some antiviral effect and is safe.</p>


Sujets)
Adulte , Sujet âgé , Femelle , Humains , Mâle , Adulte d'âge moyen , Transfert adoptif , Méthodes , Cellules cultivées , Cellules CIK , Biologie cellulaire , Allergie et immunologie , Transplantation , Fatigue , Céphalée , Hépatite B , Virologie , Cirrhose du foie , Allergie et immunologie , Thérapeutique , Transplantation autologue , Résultat thérapeutique
2.
Chinese Journal of Oncology ; (12): 465-468, 2004.
Article Dans Chinois | WPRIM | ID: wpr-254293

Résumé

<p><b>OBJECTIVE</b>To compare the inhibitory effects of cytokine-induced killer (CIK) cells alone, chemotherapeutic drug alone, and CIK cells combined with chemotherapeutic drug on the growth of hepatocellular carcinoma (HCC) cells transplanted in nude mice.</p><p><b>METHODS</b>Peripheral blood mononuclear cells (PBMC) collected from five healthy donors by blood cell separator were incubated in vitro to induce CIK cells in the presence of interferon-gamma (IFN-gamma), IL-2 and anti-CD3 monoclonal antibody (mAb). The phenotype of CIK cells was characterized by flow cytometric analysis. BEL-7402 HCC cells were inoculated subcutaneously to nude mice. On day 5, at the inoculation site were injected normal saline (group 1), CIK cells (3 x 10(7) and 6 x 10(7), group 2 and 3), mitomycin-C (MMC 80 microg in 0.2 ml, group 4), and CIK cells combined with MMC (group 5), respectively.</p><p><b>RESULTS</b>The percentage of CD3(+), CD3(+)CD8(+), CD3(+)CD56(+), CD25(+) cells increased from 64.0%, 28.0%, 7.8%, and 9.1% to 94.7%, 67.7%, 61.3%, and 84.0% respectively after cytokine induction. The percentage of CD3(+) and CD3(+)CD8(+) cells remained at high levels during incubation period, but that of CD25(+) and CD3(+)CD56(+) cells peaked respectively on day 7 and 13 and then declined. During the 90-day observation, the tumor formation rates were 100%, 70.0%, 80.0%, 70.0% and 66.7%; and the mouse survival rates were 10.0%, 60.0%, 40.0%, 50.0% and 75.0%, respectively from group 1 to group 5. Compared to the other groups, in the combined therapy group of mice, not only the tumor grew slowly and but also showed more marked tissue necrosis.</p><p><b>CONCLUSION</b>The growth inhibitory effect on human HCC transplanted in nude mice of combined CIK cells and MMC treatment is more potent than that of CIK cells or MMC alone.</p>


Sujets)
Animaux , Femelle , Humains , Mâle , Souris , Antibiotiques antinéoplasiques , Utilisations thérapeutiques , Carcinome hépatocellulaire , Allergie et immunologie , Anatomopathologie , Thérapeutique , Lignée cellulaire tumorale , Cellules cultivées , Association thérapeutique , Cytokines , Métabolisme , Pharmacologie , Immunothérapie adoptive , Cellules tueuses naturelles , Transplantation , Tumeurs du foie , Allergie et immunologie , Anatomopathologie , Thérapeutique , Souris de lignée BALB C , Souris nude , Mitomycine , Utilisations thérapeutiques , Transplantation tumorale
3.
Chinese Journal of Medical Genetics ; (6): 72-74, 2003.
Article Dans Chinois | WPRIM | ID: wpr-248494

Résumé

<p><b>OBJECTIVE</b>To investigate the single nucleotide polymorphism(SNP) loci of HIV-1 coreceptor CCR5 gene in Chinese Han people.</p><p><b>METHODS</b>The coding region of CCR5 was amplified using 2 pairs of primers and the PCR products of all 42 healthy subjects were sequenced by 4 different primers. The results of sequencing were analyzed by DNAstar in search of SNP loci.</p><p><b>RESULTS</b>Six SNP loci were discovered in the coding region of CCR5, among them four SNPs, i.e. 184A-->G, 503G-->T, 688G-->A and 999G-->T, cause amino acids changes and two SNPs are nonsense mutations. One cytosine deletion at the 894nt results in frame shift mutation and prematured termination. 184A-->G, 503G-->T and 999G-->T were found in Chinese Han people for the first time. The allelic frequencies of mutant 184G, 503T and 999T alleles were 1.1%, 21.1% and 10.0% in healthy Hans, respectively. The population distribution of G503T markedly deviated from Hardy-Weinberg equilibrium.</p><p><b>CONCLUSION</b>The SNP loci in the coding region of CCR5 in Chinese Han people has its own characteristics, which is not consistent with those of Japanese and obviously different from those of Caucasian and African.</p>


Sujets)
Adulte , Femelle , Humains , Mâle , Adulte d'âge moyen , Séquence nucléotidique , Chine , ADN , Chimie , Génétique , Analyse de mutations d'ADN , Fréquence d'allèle , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Métabolisme , Mutation ponctuelle , Polymorphisme de nucléotide simple , Génétique , Récepteurs CCR5 , Génétique , Métabolisme , Délétion de séquence
4.
Chinese Journal of Experimental and Clinical Virology ; (6): 162-164, 2003.
Article Dans Chinois | WPRIM | ID: wpr-250515

Résumé

<p><b>OBJECTIVE</b>To study the polymorphism of human immunodeficiency virus (HIV)-1 coreceptor CXCR4 in Chinese Han ethnic group for AIDS prevention and treatment.</p><p><b>METHODS</b>Totally 48 individuals were enrolled into the study. CXCR4 (cDNA No-AF147204) was cloned by PCR amplification using 2 pairs of primers, then sequenced using sequencing primers. The results of the same sequencing primers were analyzed by DNAstar software to find and identify single nucleotide polymorphism (SNP) sites.</p><p><b>RESULTS</b>Totally 7 SNPs were found in the coding region of CXCR4, among them 3 were synonymous mutation (C-->T at loci 129, 426 and 968), 3 were missense mutation (C-->T at locus 38, A-->T at locus 90, and A-->C at locus 712) and 1 was stop mutation (C-->T at 106, which converted the codon for glutamic acid into stop codon).</p><p><b>CONCLUSIONS</b>The polymorphism of CXCR4 coding region in Chinese Han is probably different from that of the other ethnic groups. Six of the 7 SNPs were discovered for the first time. Their influences on AIDS progression are worthy of studying.</p>


Sujets)
Adulte , Femelle , Humains , Mâle , Adulte d'âge moyen , Asiatiques , Séquence nucléotidique , Chine , Ethnologie , Fréquence d'allèle , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Génétique , Données de séquences moléculaires , Mutation ponctuelle , Polymorphisme de nucléotide simple , Récepteurs CXCR4 , Génétique
5.
Chinese Journal of Epidemiology ; (12): 595-598, 2003.
Article Dans Chinois | WPRIM | ID: wpr-348805

Résumé

<p><b>OBJECTIVE</b>To investigate the V249I and T280M allelic polymorphisms of human immunodeficiency virus (HIV) coreceptor CX3CR1 in HIV-1 infected and uninfected population of Chinese indigenous Han and Uygur people and to probe the association between I249-M280 haplotype and HIV-1 susceptibility as well as AIDS progression.</p><p><b>METHODS</b>Genomic DNA of 223 Uygur subjects and 316 Han subjects were purified from PBMC. I249 and M280 allelic frequencies were identified by polymerase chain reaction (PCR)/nest polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. All data were tested by chi(2) or u statistics analysis.</p><p><b>RESULTS</b>Allelic frequencies of I249 and M280 were 16.1% and 13.3% in Uygur people, and 3.3% and 2.4% in Han people. No obvious difference existed between three groups of either ethnic group. However the allelic frequencies of HIV infected population were higher than those of general population, and those of general population higher than those of HIV-1 high-risk group. There was a strong linkage between I249 and M280 (P almost zero).</p><p><b>CONCLUSIONS</b>I249 mutation was the sine qua non of M280 mutation, and most I249 alleles were accompanied by M280. The frequency of I249-M280 haplotype in Uygur population (13.3%) was adjacent to Caucasian people (15.8%), and that of I249-T280 haplotype (2.8%) was obviously lower than Caucasian people (12.5%); while both of them in Han people were much lower (0.9% and 2.4%). I249-M280 haplotype could accelerate AIDS progression according to Faure et al, while might be associated with HIV-1 susceptibility.</p>


Sujets)
Humains , Allèles , Asiatiques , Génétique , Récepteur-1 de la chimiokine CX3C , Chine , Épidémiologie , Ethnologie , Chromosomes humains de la paire 3 , Ethnies , Infections à VIH , Épidémiologie , Génétique , Virologie , VIH-1 (Virus de l'Immunodéficience Humaine de type 1) , Génétique , Haplotypes , Protéines membranaires , Génétique , Métabolisme , Mutation ponctuelle , Polymorphisme de restriction , Récepteurs aux chimiokines , Génétique , Métabolisme , Récepteur VIH , Génétique , Physiologie , Facteurs de risque
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