Diagnosis Value of Joint Detection of Critical Patients Procalcitonin and (1,3)-beta-D Glucan with Deep Fungus Infection in ICU / 现代检验医学杂志

Objective To explore the clinical application of jiont detection of critical patients procalcitomin (PCT)and (1,3)-beta D-glucan with deep fungus infection in ICU.Methods From November 2012 to August 2014 diagnosed with deep fun-gal infection of ICU critical patients,106 cases of patients with serum PCT and (1,3)-beta-D glucan content detection,and ICU of deep fungal infection in critically ill patients,519 cases were analyzed,with differencesbetween the paired t test to compare the results.Results 106 patients with deep fungal infection of ICU critical patients serum PCT for 0.701 ±0.22 pg/ml and (1,3)-beta-D glucan for 37.82±18.43 pg/ml,significantly higher than the 519 cases of ICU of deep fungal infec-tion in critically ill patients in the serum PCT for 0.238±0.12 pg/ml and (1,3)-beta-D glucan for 14.96 ±4.37 pg/ml, comparing differences between both results was statistically significant (t=7.426,8.179,P <0.05).106 patients with deep fungal infection of ICU critical patients serum PCT positive detection rate was 57.5% (61/106),significantly lower than the (1,3)-beta-D glucan positive detection rate 89.6% (95/106),difference was statistically significant (χ2 = 13.645,P <0.05).Conclusion Deep fungal infection in critical care patients in the ICU in the serum PCT and (1,3)-beta-D glucan con-tent of deep fungus infection in critically ill patients was significantly higher than the ICU,PCT and (1,3)-beta-D glucan joint detection of deep fungal infection in patients with ICU critical patients diagnosis has important clinical significance.

Outdoor (1-->3)-beta-D-glucan Levels and Related Climatic Factors / 예방의학회지

OBJECTIVES: To evaluate the monthly variation in the airborne (1-->3)-beta-D-glucan level throughout one year and its relationship with climatic factors (temperature, relative humidity, wind speed, hours of daylight, cloud cover, and pollen counts). METHODS: A total of 106 samples were collected using a two-stage cyclone sampler at five outdoor sampling locations (on top of 5 university buildings). The kinetic limulus amebocyte lysate assay was used to obtain (1-->3)-beta-D-glucan levels. RESULTS: Airborne (1-->3)-beta-D-glucan levels were significantly higher in the spring, particularly in April, and temperature was significantly related to (1-->3)-beta-D-glucan levels (r =0.339, p3)-beta-D-glucan levels may be highest in the spring, and outdoor temperature may influence (1-->3)-beta-D-glucan levels.

Evaluation of a New Diagnostic Test for Invasive Fungal Infections Using Prophenoloxydase System to Measure (1->3)-beta-D-glucan Concentration / 감염과화학요법

BACKGROUND: Prognosis of invasive fungal infection is supposed to be better when diagnosed earlier. (1->3)-beta-D-glucan (BDG) test is considered useful in early diagnosis of invasive fungal infections among high-risk patients. A new diagnostic test using prophenoloxydase system to measure BDG level is developed. A study was performed to evaluate clinical usefulness of this new diagnostic test. MATERIALS AND METHODS: 15 patients of confirmed invasive fungal infections and 38 healthy normal volunteers were enrolled. Plasma or serum BDG concentrations were measured using prophenoloxydase system. Assays for intra-run variability and inter-run variability were performed. A cut-off value was determined and sensitivity and specificity of the test were evaluated. RESULTS: A cut-off value of 94.90 pg/mL was determined. Sensitivity and specificity of the test were 86.7% and 52.6%, respectively. Statistical analyses of inter-run variability and intra-run variability revealed the test is reliable (P< or =0.001). CONCLUSION: BDG test using prophenoloxydase system is a sensitive and reliable test in non-invasive detection of invasive fungal infection.

Evaluation of a New Diagnostic Test for Invasive Fungal Infections Using Prophenoloxydase System to Measure (1->3)-beta-D-glucan Concentration / 감염과화학요법

BACKGROUND: Prognosis of invasive fungal infection is supposed to be better when diagnosed earlier. (1->3)-beta-D-glucan (BDG) test is considered useful in early diagnosis of invasive fungal infections among high-risk patients. A new diagnostic test using prophenoloxydase system to measure BDG level is developed. A study was performed to evaluate clinical usefulness of this new diagnostic test. MATERIALS AND METHODS: 15 patients of confirmed invasive fungal infections and 38 healthy normal volunteers were enrolled. Plasma or serum BDG concentrations were measured using prophenoloxydase system. Assays for intra-run variability and inter-run variability were performed. A cut-off value was determined and sensitivity and specificity of the test were evaluated. RESULTS: A cut-off value of 94.90 pg/mL was determined. Sensitivity and specificity of the test were 86.7% and 52.6%, respectively. Statistical analyses of inter-run variability and intra-run variability revealed the test is reliable (P< or =0.001). CONCLUSION: BDG test using prophenoloxydase system is a sensitive and reliable test in non-invasive detection of invasive fungal infection.

Clinical Usefulness of Serum (1-->3)-beta-D-glucan Measurement in the Diagnosis of Fungemia / 감염과화학요법

BACKGROUND: Early recognition of fungemia is essential for successful treatment. However, methods to culture fungus specimen taken from fungemia patients are difficult and time consuming. To assess the clincal usefulness of beta-D-glucan in the detection of fungemia, we compared serum (1-->3)-beta-D-glucan concentrations in fungemia, bacteremia, and healthy persons. METHODS: From August 2001 to October 2002, serum (1-->3)-beta-D-glucan concentrations were measured by turbidometric assay in 16 fungemia patients, 13 bacteremia patients and 18 healthy persons. Differences in (1-->3)-beta-D-glucan concentrations between fungemia patients and other groups were compared by t-test. RESULTS: Fungemia patients were composed of 10 male and 6 female patients, and mean age was 52.9+/-16.2 years. The cut-off value for a positive result was 11 pg/mL. thirteen out of 16 fungemia patients had concentrations above the cul-off value (range:11.5-863 pg/mL, sensitivity:81.3%, specificity:100%), and mean concentration in fungemia was 217.8+/-273.8 pg/mL. Mean concentration in bacteremia was 0.1+/-0.3 pg/mL, and all the patients with bacteremia had the concentrations below the cut-off value. Mean concentration in the healthy persons was 0 pg/mL and all healthy persons had concentration below the cut-off value. The concentration in fungemia was statistically significantly higher than those of the other two groups(p-value: respectively 0.006, 0.006) CONCLUSION: We concluded that serum (1-->3)-beta-D-glucan is useful for the diagnosis of fungemia. Further study on the usefullness of serum (1->3)-beta-D-glucan for early detection of fungemia and therapeutic monitoring is warranted.

Clinical Usefulness of Serum (1-->3)-beta-D-glucan Measurement in the Diagnosis of Fungemia / 감염과화학요법

BACKGROUND: Early recognition of fungemia is essential for successful treatment. However, methods to culture fungus specimen taken from fungemia patients are difficult and time consuming. To assess the clincal usefulness of beta-D-glucan in the detection of fungemia, we compared serum (1-->3)-beta-D-glucan concentrations in fungemia, bacteremia, and healthy persons. METHODS: From August 2001 to October 2002, serum (1-->3)-beta-D-glucan concentrations were measured by turbidometric assay in 16 fungemia patients, 13 bacteremia patients and 18 healthy persons. Differences in (1-->3)-beta-D-glucan concentrations between fungemia patients and other groups were compared by t-test. RESULTS: Fungemia patients were composed of 10 male and 6 female patients, and mean age was 52.9+/-16.2 years. The cut-off value for a positive result was 11 pg/mL. thirteen out of 16 fungemia patients had concentrations above the cul-off value (range:11.5-863 pg/mL, sensitivity:81.3%, specificity:100%), and mean concentration in fungemia was 217.8+/-273.8 pg/mL. Mean concentration in bacteremia was 0.1+/-0.3 pg/mL, and all the patients with bacteremia had the concentrations below the cut-off value. Mean concentration in the healthy persons was 0 pg/mL and all healthy persons had concentration below the cut-off value. The concentration in fungemia was statistically significantly higher than those of the other two groups(p-value: respectively 0.006, 0.006) CONCLUSION: We concluded that serum (1-->3)-beta-D-glucan is useful for the diagnosis of fungemia. Further study on the usefullness of serum (1->3)-beta-D-glucan for early detection of fungemia and therapeutic monitoring is warranted.