Study on active components and mechanism of shikonin in prevention and treatment of blood heat syndrome / 中国药理学通报

Aim To systematically evaluate the heat-clearing mechanism of Arnebiae Radix on two mouse models of blood heat syndrome. Methods The drug-forming molecules were screened by comprehensive network pharmacology methods, and the correlation between drug efficacy and related factors and targets was evaluated on the mouse model of short effect blood heat syndrome constructed by 2, 4-dinitrophenol (DNP) and the mouse model of severe blood heat syndrome (heat stroke) constructed by high temperature combined with lipopolysaccharide (LPS). Results A total of 277 shikonin related targets were collected, mainly involving biological processes such as inflammatory reaction, oxidation reaction and coagulation reaction. Shikonin, a representative compound, significantly improved the main syndromes of mice with blood heat syndrome, reduced the levels of inflammatory factors IL-1β, IL-6 and TNF-α in the two models, and reduced the contents of oxidative damage indexes LPO and MDA, and the two showed correlation. The main mechanism was to inhibit the expression of NF-ΚB p65 and up-regulate the expression of Nrf2. Conclusions Shikonin plays a pharmacological role in the prevention and treatment of blood heat syndrome by inhibiting inflammation and improving antioxidant capacity, which provides a pharmacological basis for shikonin in the prevention and treatment of blood heat syndrome.

Relationship between clearing heat and cooling blood and regulating blood redox homeostasis of shikonin based on blood heat syndrome mice model / 中草药

Objective: To explore the relationship between clearing heat and cooling blood of shikonin (SK) and regulation of blood redox homeostasis in blood-heat syndrome mice model induced by active yeast. Methods: Mice were randomly divided into control group, model group, SK (10 mg/kg) group, aspirin positive (200 mg/kg) group, L-buthionine sulfoximine (BSO, 600 mg/kg) group, N-acetyl-L-cysteine (NAC, 400 mg/kg) group, BSO combined with SK group, NAC combined with SK group. Mice were sc with 0.2 g/mL active yeast (10 mL/kg) on the neck, mice were ip SK at 0.5 h, ig Asp at 8 h, ip BSO and NAC before the beginning of the experiment (twice a day for one week). The rectal temperature (tR), general activity, metabolism, coagulation, and blood cell counting were determined. The levels of GSH and GSSG were determined by OPA assay. Results: Compared with the control group, tR of mice was significantly increased at 4 h (P < 0.05) and reached (38.07 ± 0.11)℃ at 10 h in model group; Irritability, redder lips, inclined to drinking, reduced carbon powder propelling rate, decreased pellet moisture capacity and prolonged coagulation time were observed (P < 0.05); RBC and inflammatory cells infiltrated were observed in lung tissue sections; The levels of LPO and MDA in serum were significantly increased, the activity of SOD was decreased, GSH/GSSG was decreased (P < 0.05). Compared with model group, tR of mice was significantly reduced and reached (37.51 ± 0.12)℃ at 10 h in SK group; The symptoms were significantly attenuated; The level of LPO and MDA in serum were decreased; The activity of SOD and GSH/GSSG ratio were increased (P < 0.05). BSO significantly reduced the GSH/GSSG ratio (P < 0.05), reduced the effect of SK on blood-heat syndrome mice model. NAC increased the level of GSH in serum and GSH/GSSG ratio (P < 0.05), enhanced the SK treatment effect. Conclusion: SK can attenuate hyperpyrexia, hemorrhage and congestion, oxidative damage in blood-heat syndrome mice model by regulating the blood redox homeostasis, and thereby the effect of clearing heat and cooling blood work.

Study on clinical efficacy and mechanism of Qingying Tang for treating psoriatic blood-heat syndrome based on IL-23/Th17 / 中国中药杂志

To observe the clinical curative effect of Qingying Tang in the treatment of psoriatic blood-heat syndrome and explore its intrinsic mechanism.In this study,we collected 72 patients with blood-heat syndrome psoriasis admitted to our dermatology clinic from January 2016 to December 2017 and divided into control group and observation group according to the random number table method,36 cases in each group.The patients in control group were given with Acitretin Capsules orally,10 mg/time,twice a day.The patients in observation group were given with Qingying Tang orally,150 mL/time,twice a day.The treatment period was 12 weeks in both groups.The traditional Chinese medicine(TCM) syndrome scores before and after treatment,psoriasis area and severity index(PASI) score,dermatology life quality index(DLQI) score,and the clinical efficacy of the two groups were compared between the two groups;flow cytometry was used to detect peripheral blood Th17 cell percentages before and after treatment in both groups;serum interleukin(IL)-17,IL-23,IL-22,and IL-21 levels in both groups before and after treatment were measured by ELISA;the expression levels of STAT3 and RORγt before and after treatment in patients were measured by using skin lesion immunohistochemical method.The results showed that the TCM symptoms were improved significantly in both groups(P<0.05),and the effect in observation group was significantly better than that in the control group(P<0.05).PASI and DLQI scores were decreased significantly after treatment in both groups(P<0.05),and the scores in observation group were significantly lower than those in the control group(P<0.05).The curative effect of the observation group was significantly higher than that of the control group(P<0.05).After treatment,the percentage of Th17 cells,as well as IL-17,IL-23,IL-22 and IL-21 levels in peripheral blood were significantly decreased in both groups(P<0.05),and the levels in observation group were significantly lower than those in the control group(P<0.05).The expression levels of STAT3 and RORγt in both groups were significantly lower than those before treatment(P<0.01),and the levels in observation group were significantly lower than those in the control group(P<0.05).All of the results indicted that Qingying Tang can effectively improve the skin lesions and TCM syndrome in patients with psoriasis and blood-heat syndrome,and improve patient health quality,which may be related to regulation of peripheral blood IL-23/Th17.

Clinicla Research onQingre Huoxue Mixture in Treating Blood Heat Type Psoriasis Vulgaris / 中国中医药信息杂志

Objective To investigate the efficacy and safety ofQingre Huoxue Mixture in the treatment of blood heat type psoriasis vulgaris.Methods The patients were divided into treatment group and control group according to random number table method, with 52 cases in treatment group and 50 cases in the control group, 10 cases failed to complete the trial. Treatment group was givenQingre Huoxue Mixture, and the control group received oral administration of compoundQingdaiCapsule. The treatment lasted for 8 weeks. PASI score, TCM syndrome integrals, DLQI score, serum IFN-γ and IL-10 were detected, and hematuria routine and liver and kidney function were under safety testing before and after treatment.Results After treatment, PASI score, DLQI score and TCM syndrome integrals in the treatment group decreased significantly (P<0.001); the expression of serum IFN-γ decreased significantly (P<0.001); the expression of serum IL-10 increased significantly (P<0.001), with the total effective rate of 100% (52/52). After the treatment, TCM syndrome integrals in the both groups decreased significantly (P<0.001). After the treatment, the rash burning and itching scores in the treatment group were lower than the control group, with statistical significance (P<0.001). In terms of safety, the treatment group had no abnormal indicators.ConclusionQingre Huoxue Mixture has obvious curative effect, which can reduce the patients with psoriasis, improve the life quality of patients, and improve the clinical symptoms of rash burning and itching, reduce serum IFN-γ, and increase serum IL-10.

Causal analysis of blood heat syndrome in psoriasis vulgaris / 中华中医药杂志

Objective:To analyze the cause of the blood heat syndrome in psoriasis.Methods: Made the investigation form about information of psoriatic patients through DME method and reference both Chinese and foreign documents,analyzed main component of data by SAS software.Results: The first main component of blood heat syndrome in psoriasis are infection,drinking,dietetic problem,and emotion;the second main component is family history;the third main component is dampness;the forth main component is the allergy to the food;the fifth is outside irritation.Through the corresponding investigation we found that food include hot food,beef,sheep and sea food,emotion problem include over work and stress,dampness include work and living in humid place for long time,outside stimulus meant chemical stimulus.Conclusion: The main cause of the blood heat syndrome in psoriasis are infection,drinking,dietetic problem,and emotion,family history,humidity,allergy to the food and chemical stimulus in order.

Clinical Observation on Xiaoyin Ointment for the Treatment of Blood-heat Syndrome of Psoriasis / 中医杂志

Objective To evaluate the effect and safety of Xiaoyin Ointment for blood-heat syndrome of psoriasis and to study further its effect on single symptoms of psoriasis such as erythema,scale,infiltration,pruritus and lesion area. Methods The 99 selected patients were randomized into 3 groups and were treated by Xiaoyin Ointment,Shidu (Damp and toxin removal) Ointment,and Vaseline Ointment respectively for 8 weeks. All patients of 3 groups took the Keyin Yifang (First prescription for psoriasis) as the basic medication. After treatment,the general effect and the effect on skin lesion were evaluated objectively,and the time-effect indices and the safety were assessed as well. Results The total effective rate(93.9%) of Xiaoyin Ointment on blood-heat syndrome was significantly superior to that of Shidu Ointment (78.8%,P