Chemical Constituents from Caulophyllum robustum (Ⅱ) / 中国药学杂志

OBJECTIVE: To isolate the chemical constituents from Caulophyllum robustum and confirm their chemical structures. METHODS: The chemical constituents were isolated by MCI gel, repeated silica gel chromatography, preparative liquid chromatography.and their structures were elucidated by NMR and MS etc. RESULTS: The structures of compounds 1-10 were identified as echinocystic acid (1), oleanolic acid-3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranoside (2), hederagenin-3-O-β-D-glucopyranosyl-(1→3)-α-L-arabinopyranoside (3), hederagenin-3-O-β-D-glucopyranosyl-(1→2) [β-D-glucopyranosyl-(1→3)]-α-L-arabinopyranoside (4), 3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl echinocystic acid-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (5), 3-O-α-L-arabinopyranosyl hederagenin-28-O-(4-O-acetyl)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (6), (6R, 7E, 9R)-9-hydroxy-4, 7-megastigmadien-3-one-9-O-β-D-glucoside (7), (9R)-9-hydroxy-4, 6-megastigmadien-3-one-9-O-β-D-glucoside (8), maltose (9), and sucrose (10). CONCLUSION: Compounds 1-10 are firstly isolated from the genus Caulophyllum except 5.

Chemical constituents of Caulophyllum robustum and its antitumor activities / 中草药

Objective To study the chemical constituents of Caulophyllum robustum and its antitumor activities. Methods The chemical constituents were isolated and purified with silica gel column chromatography, dextran gel Sephadex LH-20, and other chromatographic methods. Their structures were determined by physicochemical and spectral analysis. Furthermore, the cytotoxicity of these chemical components against the NSCLC A549 cell line was measured by CCK-8 method. Results Eleven compounds were isolated and identified as collinsogenin (1), hederagenin (2), eechinocystic acid 3-O-α-L-arabinopyranoside (3), magnolamide (4), (-)-magnocurarine (5), columbamine (6), 4,4’-diphenylmethane-bis (methyl) carbamate (7), (-)-8,8’-dimethoxy-L-O-(β-D- glucopyranosyl) secoisolariciresinol (8), asechipuroside A (9), 2-[4-(3-hydroxy-1-propenyl)-2-methoxyphenoxy]-1,3-propanediol (10), and 1-linoleoylglycerol (11). Conclusion Compounds 1-2 and 4-11 are isolated from Caulophyllum robustum genus for the first time. Compounds 1 and 2 show moderate inhibited effect on the proliferation of NSCLC A549 cell line.

Determination of six saponins in Caulophyllum robustum by QAMS / 中草药

Objective: To establish a method of quantitative analysis of multi-components by single-marker (QAMS) for simultaneous determining six saponins in Caulophyllum robustum. Methods: Cauloside G was used as the internal reference standard; The relative correction factors of cauloside H, leonticin D, cauloside D, cauloside B, and cauloside C in C. robustum were calculated. To achieve the QAMS, the contents of indicative saponins were calculated by relative correction factors. At the same time, the external standard method was used to detect the contents of six kinds of ingredients in medicinal materials, and feasibility of QAMS was verified. Results: The relative correction factor had good reproducibility, there were no significant differences among the calculated values of multi-evaluation and measured values of the external standard method. Conclusion: In the absence of reference substance situation, QAMS can be used for the quality evaluation of C. robustum, and the method is accurate and reliable.

Saponin constituents from roots and rhizomes of Caulophyllum robustum / 中草药

Objective: To separate the saponins from the roots and rhizomes of Caulophyllum robustum and determine their chemical structures. Methods: The chemical constituents were isolated by repeated silica gel chromatography, medium pressure column chromatography, and semi-preparative liquid chromatography. Their structures were elucidated by the data of NMR and MS. Results: Ten compounds were isolated from the roots and rhizomes of C. robustum and the structures of compounds 1-10 were identified as echinocystic acid-3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranoside (1), 3-O-α-L-arabinopyranosylhederagenin-28-O-β-D- glucopyranosyl-(1→6)-β-D-glucopyranoside (2), HN-saponin H (3), ciwujianosides A1 (4), glycoside L-K1 (5), 3-O-β-D- glucopyranosyl-(1→3)-α-L-arabinopyranosyl-hederagenin-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-gluco-pyranoside (6), leonticin F (7), 3-O-β-D-glucopyranosyl-(1→3) [β-D-glucopyranosyl-(1→2)] α-L-arabinopyranosyl-echinocystic acid-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (8), leonticin A (9), and morroniside (10). Conclusion: Compound 10 is a iridoid. Compounds 1 and 10 are firstly isolated from the plants of Caulophyllum Maxim., compouds 2-9 are isolated from this plant for the first time.