Résumé
Objective: To study the chemical constituents in the twigs of Cassia fistula. Methods: The chemical constituents in the twigs of C. fistula were isolated by silica gel, Sephadex LH-20 column chromatography, and RP-HPLC methods. And their structures were elucidated by using various spectroscopic techniques, including extensive 1D and 2D NMR techniques. Results: A new tricyclic alkaloid, named as cassiarin L (1) was isolated from 95% ethanol extract of the twigs of C. fistula. Conclusion: Compound 1 is a new compound and it displays obvious cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 1.8, 2.6, 3.5, 4.2, and 2.3 μmol/L, respectively.
Résumé
A new isoindole alkaloid (1), has been isolated from the leaves of Cassia siamea by using various chromatographic techniques. Compound 1 is a new compound, determined as 5-(hydroxymethyl)-2-methyl-6-prenylisoindolin-1-one, and it displayed cytotoxicity against NB4, A549, SHSY5Y, PC3 and MCF7 cell lines with IC₅₀ values of 3.2,4.6,2.8,6.4, 2.5 μmol•L⁻¹, respectively.
Résumé
Objective: To study the chemical constituents in the twigs of Cassia siamea. Methods: The chemical constituents in the twigs of C. siamea were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatographies and RP-HPLC methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results: A new anthraquinone, named as cassanthraquinone A (1) was isolated from 95% ethanol extract of the twigs of C. siamea. Conclusion: Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 6.2, 7.8, 3.5, 8.6, and 5.4 μmol/mL, respectively.