Résumé
Objective To investigate the effect of recombinant Vibrio vulnificus hemolysin (rVvhA) on the expression of nitric oxide(NO) and induced nitric oxide synthase(iNOS) in J774A. 1 cells.Methods The inhibitory effect of rVvhA on J774A. 1 proliferation was measured by MTF colorimetry technique. The content of nitrite in culture medium was determined by Griess reagent. The expression of iNOS protein and mRNA were measured by immunofluorescence and RT-PCR, respectively. Results The viability of J774A. 1 cells was apparently inhibited when exposed to 0.8 HU/ml rVvhA and up. With the help of IFN-γ, the expression of NO and the activity of iNOS in J774A. 1 cells were remarkably increased when exposed to 0.4 HU/ml rVvhA. Conclusion rVvhA can increase the expression of NO and iNOS, it may play an important role in the pathogenic mechanism of Vibrio vulnificus.
Résumé
Objective:To observe the effects of fuyuan capsule on change of morphology of synovium,expression of IL-1?、MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits,and investigate the mechanism about the reparation and treatment of fuyuan capsule.Methods:Sixty New Zealand rabbits were divided into five groups randomly:normol group,model contrast group,low dose group, middle dose group and high dose group of fuyuan capsule.OA model was induced in rabbits by improved Hulth'S method.Three Chinese herb groups were treated with fuyuan capsule(0.727,1.453,4.795 g(/kg?d)),continuously for 4,8 weeks and 12 weeks,model contrast group was treated with 0.9%NS,ig,at the same time.Synovium sections were analyzed by immunohistochemistry for the IL-1?,MMP-13 and iNOS,observation and measurement of the change of morphology of synovium by light microscopic and transmission electron microscopic.After the experiment,the differences of these indexes between Three Chinese herb groups and model contrast group were compared.Results:There are found significant differences about measurement of morphology of synovium between Three Chinese herb groups and model contrast group after the experiment(P