Chemical constituents of Patrinia villosa / 中草药

Objective: To study the chemical constituents of Patrinia villosa. Methods: Compounds were isolated by a combination of various chromatographic techniques including column chromatography over macroporous resin, Sephadex LH-20, and reversed-phase HPLC. Their structures were elucidated by physiochemical property and spectral analysis. Results: Fourteen compounds were isolated and identified as isololiolide (1), citroside A (2), grasshopper kectone (3), (E)-4-Hydroxy-3,3,5-trimethy1-4- (3-oxobut-1-en-1-yl)-cyclohexan-1-one (4), bluemenol A (5), pubinernoid A (6), robinin (7), puerarin (8), 5-(1'-hydroxyethyl)-methyl nicotinate (9), 2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-propance-1,3-diol (10), 1-O-(β-D-glucosyl)-2-[2-methoxy-4-(3-hydroxypropyl)- phenoxy]-propan-3-ol (11), dihydrosinapyl alcohol (12), 3,5-dimethoxyl-4-hydroxyl-phenylpropanol-9-O-β-D-glucopyranoside (13), and 2-phenylethy-α-L-arabinopyranosyl-(1″→6')-β-D-glucopyranoside (14). Conclusion: All compounds are isolated from the plants of Patrinia Juss. for the first time.

Chemical constituents of stems and leaves of Clausena hainanensis / 中草药

Objective To study the chemical constituents of the stems and leaves of Clausena hainanensis. Methods The chemical constituents of C. hainanensis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results Eighteen compounds were isolated from the 90% ethanol extract of the stems and leaves of C. hainanensis, which were identified as mukonine (1), methyl carbazole-3-carboxylate (2), lansine (3), murrayanine (4), 3-formylcarbazole (5), 3-formyl-6-methoxycarbazole (6), lansamide 4 (7), 4-methoxy-N-methyl-2-quinolone (8), (E)-N- (4-methoxyphenethyl)-2-methylbut-2-enamide (9), (E)-N-methyl cinnamon amide (10), N-(2-hydroxy-2-phenylethyl) cinnamamide (11), N-benzoyltyramine (12), aurantiamide (13), N-methyl-2-pyrolidinone (14), coumaric acid (15), 6,8-dimethoxy-4,5-dimethyl-3- methyleneisochromanl-one (16), 8-methoxypsoralen (17), and isololiolide (18). Conclusion This is the first time reporting the chemical constituents from C. hainanensis, all compounds are isolated from C. hainanensis for the first time, and compounds 12-16 are isolated from the genus Clausena for the first time.