Association of lipoprotein lipase (LPL) gene variants with hyperlipidemic acute pancreatitis in southeastern Chinese population

ABSTRACT Objective: The study aims to explore the relationship between lipoprotein lipase (LPL) variants and hyperlipidemic acute pancreatitis (HLAP) in the southeastern Chinese population. Subjects and methods: In total, 80 participants were involved in this study (54 patients with HLAP and 26 controls). All coding regions and intron-exon boundaries of the LPL gene were sequenced. The correlations between variants and phenotypes were also analysed. Results: The rate of rare LPL variants in the HLAP group is 14.81% (8 of 54), higher than in controls. Among the detected four variants (rs3735959, rs371282890, rs761886494 and rs761265900), the most common variant was rs371282890. Further analysis demonstrated that subjects with rs371282890 "GC" genotype had a 2.843-fold higher risk for HLAP (odds ratio [OR]: 2.843, 95% confidence interval [CI]: 1.119-7.225, p = 0.028) than subjects with the "CC" genotype. After adjusting for sex, the association remained significant (adjusted OR: 3.083, 95% CI: 1.208-7.869, p = 0.018). Subjects with rs371282890 "GC" genotype also exhibited significantly elevated total cholesterol, triglyceride and non-high-density lipoprotein cholesterol levels in all the participants and the HLAP group (p < 0.05). Conclusion: Detecting rare variants in LPL might be valuable for identifying higher-risk patients with HLAP and guiding future individualised therapeutic strategies.

A case of neonatal-onset type I hyperlipoproteinemia with bloody ascites / 中国当代儿科杂志

This report presents a case of a male infant, aged 32 days, who was admitted to the hospital due to 2 days of bloody stools and 1 day of fever. Upon admission, venous blood samples were collected, which appeared pink. Blood biochemistry tests revealed elevated levels of triglycerides and total cholesterol. The familial whole genome sequencing revealed a compound heterozygous variation in the LPL gene, with one variation inherited from the father and the other from the mother. The patient was diagnosed with lipoprotein lipase deficiency-related hyperlipoproteinemia. Acute symptoms including bloody stools, fever, and bloody ascites led to the consideration of acute pancreatitis, and the treatment involved fasting, plasma exchange, and whole blood exchange. Following the definitive diagnosis based on the genetic results, the patient was given a low-fat diet and received treatment with fat-soluble vitamins and trace elements, as well as adjustments to the feeding plan. After a 4-week hospitalization, the patient's condition improved and he was discharged. Follow-up showed a decrease in triglycerides and total cholesterol levels. At the age of 1 year, the patient's growth and psychomotor development were normal. This article emphasizes the multidisciplinary diagnosis and treatment of familial hyperlipoproteinemia presenting with symptoms suggestive of acute pancreatitis, including bloody ascites, in the neonatal period.

Association of the lipoprotein lipase and Apolipoprotein C-II gene polymorphisms with risk of dyslipidemia in smokers and non-smokers male

Objective: Dyslipidaemia is considered a metabolic abnormality andan important risk factor that leads to atherogenic cardiovascular diseases. Cigarette smoking is associated with dyslipidaemia. This study aimed to demonstrate whether lipoprotein lipase enzyme (LPL) and Apolipoprotein CII (APOCII) gene polymorphisms can be considered as independent genetic risk factors for dyslipidaemia among smokers with various smoking durations. Methods: A total of 185 males (90 smokers and 95 non-smokers)were included in this study, Lipid profiles were measured and DNA was isolated. The LPL-Hind III and APO CII-Ava II polymorphisms were determined using the polymerase reaction-restriction fragment length polymorphisms (RFLP) technique. Results: For the LPL-Hind IIIpolymorphism HþHþ genotype group, the triglycerides TG and very-lowdensity lipoprotein cholesterol VLDL-C concentrations were significantly higher and the high-density lipoprotein cholesterol HDL-C concentration was significantly lower than those of the HeH- genotype. ForAPO CII-Ava II polymorphisms, compared with those of the A2A2 genotype group, the total cholesterol TC, TG, low-density lipoprotein cholesterol LDL-C and VLDL-C concentrations were significantly increased in the A1A2 genotype group, while the HDL-C concentration was significantly decreased. Conclusions: The study revealed that the HþHþ or H þ H-genotype of the LPL-Hind III polymorphism and the A1A1or A1A2 genotype of the APOCII-Ava II polymorphism were at higher risk of developing dyslipidaemia compared to the HeH- genotype of the LPL-Hind III polymorphism and A2A2 genotype of the APOCII-Ava II polymorphism.

Improvement effect and mechanism of ethanol extract from Citri Reticulatae Pericarpium on triglyceride in hyperlipidemia model rat / 中国中药杂志

The aim of this paper was to study the improvement effect of ethanol extract from Citri Reticulatae Pericarpium(CRP) on triglyceride of hyperlipidemia model rats, and to explore the possible mechanism. SD rats were randomly divided into normal group, model group, positive control group, and high, medium and low-dose CRP ethanol extract groups, with 10 rats in each group. During the experiment, except for the normal group that was fed with distilled water and ordinary feed, rats in the other groups were given different concentrations of alcohol and fed with high-sugar and fat diets. All rats were given free diets. While being modeled, each group was administered with 0.01 mL·g~(-1) by gavage once a day for six weeks. Blood samples were collected after two weeks, four weeks and six weeks of drug treatment. After the completion of the experiment, blood, liver and adipose tissue were collected. Triglyceride(TG), alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) in serum, TG in liver tissue and TG in fecal were detected. Free fatty acid(FFA) and triglyceride-related hydrolase, such as adipose tiglyceride lipase(ATGL), lipoprotein lipase(LPL), hepatic lipase(HL), hormone-sensitive triglyceride lipase(HSL) were detected by ELISA. The mRNA expressions of peroxisome proliferators-activated receptors(PPARγ), sterol regulatory element binding protein 1 c(SREBP-1 c) and farnesoid X receptor(FXR) were determined by RT-PCR. Compared with the model group, each administration group could reduce TG levels in serum and liver to varying degrees, reduce serum ALT, AST, ALP activities, significantly reduce free fatty acid content in serum, significantly increase triglyceride metabolism-related enzymes, including fat ATGL, LPL and liver HL content, and significantly reduced the content of fat HSL. According to the study of transcriptional regulation genes relating to triglyceride metabolism, extract from CRP could significantly increase the mRNA expressions of PPARγ and FXR. In conclusion, ethanol extract from CRP could ob-viously reduce the TG level of hyperlipidemia model rats, and might reduce plasma TG content by increasing PPARγ-LPL/ATGL and FXR-HL triglyceride hydrolysis pathways.

Identification of differentially expressed genes and pathways in lipoprotein lipase gene heterozygous knockout mice through gene microarray analysis / 上海交通大学学报（医学版）

Objective • To screen the differentially expressed genes (DEGs) and pathways in the islet tissues of lipoprotein lipase (Lpl) gene heterozygous knockout (Lpl+/-) mice and wild type (WT) mice, and explore the molecular mechanism of pathogenesis of type 2 diabetes mellitus (T2DM) mediated by lipotoxicity. Methods • The islets of Lpl+/- mice and WT mice were isolated and purified. DEGs were screened by gene microarray analysis. Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DEGs were performed. The expressions of key genes were verified by quantitative real-time PCR (qPCR). Results • A total of 187 DEGs were identified. GO functional analysis and KEGG pathway analysis showed that DEGs were mainly involved in the biological processes such as immune cell proliferation and differentiation, inflammatory signaling pathways and cell adhesion. Among the top 10 DEGs screened from Lpl+- mice and WT mice, gremlin 1 (Grem1) gene was closely related to the function of islet β cells, while the result of qPCR was consistent with that of gene microarray analysis. Conclusion • Multiple signaling pathways are involved in the process of T2DM mediated by lipotoxicity, which may lead to the dysfunction of islet β cells by inhibiting Grem1 expression.

MicroRNA-138 Suppresses Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting Lipoprotein Lipase

PURPOSE: Adipogenic differentiation of adipose tissue-derived mesenchymal stem cells (AMSCs) is critical to many disease-related disorders, such as obesity and diabetes. Studies have demonstrated that miRNA-138 (miR-138) is closely involved in adipogenesis. However, the mechanisms affected by miR-138 remain unclear. This work aimed to investigate interactions between miR-138 and lipoprotein lipase (LPL), a key lipogenic enzyme, in AMSCs. MATERIALS AND METHODS: Human AMSCs (hAMSCs) isolated from human abdomen tissue were subjected to adipogenic differentiation medium. Quantitative real-time polymerase chain reaction and Western blot assay were applied to measure the expressions of miR-138, LPL, and the two adipogenic transcription factors cytidine-cytidine-adenosine-adenosine-thymidine enhancer binding protein alpha (C/EBPα) and peroxisome proliferator-activated receptor gamma (PPARγ). The relationship between miR-138 and LPL was predicted utilizing the miRTarBase database and validated by dual luciferase reporter assay. RESULTS: Showing increases in C/EBPα and PPARγ expression levels, hAMSCs were induced into adipogenic differentiation. During adipogenesis of hAMSCs, miR-138 expression was significantly downregulated. Overexpression of miR-138 by transfection inhibited hAMSCs adipogenic differentiation in vitro. Mechanically, LPL was a target of miR-138. LPL expression was upregulated during adipogenesis of hAMSCs, and this upregulation was reversed by miR-138 overexpression. Functionally, silencing of LPL by transfection exerted similar inhibition of the expressions of C/EBPα and PPARγ. Meanwhile, LPL ectopic expression was able to partly abolish the suppressive effect of miR-138 overexpression on adipogenic differentiation of hAMSCs. CONCLUSION: Upregulation of miR-138 inhibits adipogenic differentiation of hAMSCs by directly downregulating LPL.

Variants of Lipolysis-Related Genes in Korean Patients with Very High Triglycerides

We investigated the prevalence and characteristics of variants of five lipolysis-related genes in Korean patients with very high triglycerides (TGs). Twenty-six patients with TG levels >885 mg/dL were selected from 13545 Korean subjects. Five candidate genes, LPL, APOC2, GPIHBP1, APOA5, and LMF1, were sequenced by targeted next-generation sequencing. Predictions of functional effects were performed and matched against public databases of variants. Ten rare variants of three genes were found in nine (34.6%) patients (three in LPL, four in APOA5, and three in LMF1). Five were novel and all variants were suspected of being disease-causing. Nine were heterozygous, and one (3.8%) had a homozygous rare variant of LPL. Six common variants of four genes were observed in 25 (96.2%) patients (one in LPL, one in GPIHBP1, two in APOA5, and two in LMF1). The c.G41T variant of GPIHBP1 and c.G533T variant of APOA5 were most frequent and found in 15 (57.7%) and 14 (53.8%) patients, respectively. Rare homozygous variants of the genes were very uncommon, while diverse rare heterozygous variants were commonly identified. Taken together, most study subjects may be manifesting the combined effects of rare heterozygous variants and common variants.

Hypolipidemic Activity Of Cassia Tora Seeds In Hyperlipidemic Rats

The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds extract has been studied in two hyperlipidemic models of rat. These are triton injected and cholesterol rich HFD fed model of hyperlipidemia. In triton WR-1339 induced hyperlipidemia, feeding with root extract (500 mg/ kg body wt/ day p.o. ) exerted lipid lowering effect as assessed by reversal of plasma levels of total cholesterol (TC), phospholipids (PL), triglyceride (TG) and reactivation of Post Heparin Lipolytic Activity (PHLA) of plasma. The other model was fed with cholesterol rich HFD and seeds extract of Cassia tora (500 mg/ kg body wt/ day p.o.) simultaneously for 30 days. This also caused lowering of lipid levels in plasma and liver homogenate and reactivation of plasma post heparin lipolytic activity, hepatic total lipoprotein lipase activity. The hypolipidemic activity of Cassia tora seeds was compared with a standard drug guggulipid (200 mg/ kg body wt/ day p.o.), a known lipid lowering drug in both models.

Inhibitory effects of Capsicum annuum L. water extracts on lipoprotein lipase activity in 3T3-L1 cells

Obesity, an intractable metabolic disease, currently has no medical treatment without side effects, so studies have been actively carried out to find natural compounds that have anti-obesity activity with minimum side effects. In this study, the anti-obesity effects of water extracts of seven Capsicum annuum L. varieties being Putgochu (Pca), Oyee gochu (Oca), Kwari putgochu (Kca), Green pepper (Gca), Yellow paprika (Yca), Red paprika (Rca) and Cheongyang gochu (Cca), were examined through the evaluation of lipoprotein lipase (LPL) mRNA expression level in 3T3-L1 cells (mouse pre-adipocytes). After capsaicin elimination by chloroform defatting, freeze-dried powder of Cca was treated to 3T3-L1 cells and anti-obesity effects were examined by determining the LPL mRNA level using the RT-PCR method. Of the primary fractions, only proven fractions underwent secondary and tertiary refractionating to determine anti-obesity effects. From seven different Capsicum annuum L., there was a significant decrease of the LPL mRNA expression level of 50.9% in Cca treatment compared to the control group. A significant decrease of the LPL mRNA expression level was shown in primary fractions (Fr) 5 (36.2% decrease) and 6 (30.5% decrease) of the Cca water extracts. Due to the impurities checked by UPLC chromatography, Fr 5 and 6 were refractionated to determine the LPL mRNA expression level. Treatment of Fr 6-6 (35.8% decrease) and Fr 5-6 (35.3% decrease) showed a significant decrease in the LPL mRNA expression level. When analyzed using UPLC, major compounds of Fr 6-6 and Fr 5-6 were very similar. Subsequently, we refractionated Fr 6-6 and Fr 5-6 to isolate the major peak for structure elucidation. Treatment of Fr 5-6-1 (26.6% decrease) and Fr 6-6-1 (29.7% decrease) showed a significant decrease in the LPL mRNA expression level. Consequently, the fractions may have a possibility to ameliorate obesity through the decrease of the LPL mRNA expression level.

Study of total flavonoid in Ligustrum Lucidum Ait on lipometabolism regulation of HepG2 cell / 国际中医中药杂志

Objective To study molecular target of total flavonoid in Ligustrum Lucidum Ait(TFL) on lipometabolism regulation of HepG2 cell.Methods Treatment doses of total flavonoid in Ligustrum Lucidum Ait were determined by MTT and LDH.The expression of lipoprotein lipase(LPL),3-hydroxy-3-methylglutary-coenzyme A redutasein(HMGCR)and peroxisome proliferator-actived receptorsα(PPARα)mRNA of HepG2 cell were determined by reversed transcription polymerase chain reaction(RT-PCR).Results Increased expression of LPL mRNA and PPARαmRNA with TFL(10-6～10-4g/ml)were found in HepG2 cells after treated with TFL(10-5g/ml),but decreased expression of HMGCR mRNA was found after treated with TFL(10-5,10-4 g/ml).Conclusion The potential mechanisms of total flavonoid in Ligustrum Lucidum Ait's hypolipidmic effects may be with its functions of regulating lipoprotein degradation through PPARα-LPL pathway,or regulating steroid biosynthesis by reducing HMGCR.

Association of Polymorphism of Lipoprotein Lipase Gene with Type 2 Diabetes and the Lipids Spectrum in Uygurs / 中国慢性病预防与控制

Objective To study the association of LPL gene polymorphism with type 2 diabetes and the lipids spectrum in Uygurs. Methods Based on the case-siblings control design, the lipid spectrum of Uygur were tested by Automatic biochemical analyzer and the polymorphism of LPL gene were analyzed by RLFP with Hind Ⅲ in 62 T2DM patients, 62 IGT patients and 124 normal controls of Uygurs. Results The genotype distribution and allele frequencies of LPL gene in three groups were not statistically significant. The average of TG in H+H+group, H-H-group and H+H-group were 2.26, 1.73 and 1.80 mmol/L; Compared with the three genotypes and lipid indicators, TG content of Mutant H +H + group were higher than that in other groups. Multi-factor Logistic regression analysis showed T2DM was closely related to TG (P=0.034)and waist circumference (P=0.001). Conclusion The relation between LPL gene polymorphism by Hind Ⅲ and the risk for T2DM in Xinjiang Uygur population are no statistical relevance, LPL gene mutations may be one of the factors causing elevated levels of plasma TG.

The role of Akt and Lpl in formation of nonalcoholic steatohepatitis in rat with a fat-rich diet / 中国康复理论与实践

@#ObjectiveTo investigate the role of serine/threonine kinase (Akt) and lipoprotein lipase (Lpl) in formation of nonalcoholic steatohepatitis (NASH) in rat with a fat-rich diet.Methods40 male Wistar rats were randomly divided into the 8-week-rich-fat group (n=10), 8-week-common-diet control group (n=10), 12-week-rich-fat group (n=10) and 12-week-common-diet control group (n=10). The lipid, cholesterol, glucose and insulin were examined, insulin resistance index (IRI) and insulin sensitivity index (ISI) were calculated, the expression of Akt and Lpl were detected with immunohistochemical method and morphological changes of liver were observed by transmission electron microscope (TEM).ResultsNASH was formed in each rat of 8-week-rich-fat group with characteristics of individual obese, increased liver volume, full and clear contour, gray-yellowish luster, greasy section and crisp quality. And it was accompanied by high lipoidemia (HL), liver fat cell denaturation, inflammatory cell infiltration in liver lobuler and cell death in liver. The expression of Akt was obviously reduced and the expression of Lpl was obviously weakened in liver. Lipid, cholesterol, glucose and insulin and IRI were gradually advancing. ISI was reducing and the insulin resistance formed. The data of fat-rich diet groups was significantly different with that of common-diet control groups (P<0.0001).Conclusion Steatohepatitis and insulin resistance can form in rat by feeding with rich-fat diet for 8 weeks. It causes the insulin and Lpl activeness reducing, and steatolysis barrier.

Progress on the drugs for regulating high-density lipoprotein / 中国临床药理学与治疗学

Atherosclerosis (AS) is pathologically important basis of many kinds of coronary atherosclerosis disease (CAD). It can be substantially protected by raising high-density lipoprotein (HDL).In view of mechanism, drugs for raising HDL include: cholesterol ester transfer protein inhibitors, peroxisomal proliferator-activated receptor agonists, liver X-activated receptor agonists, farnesoid X receptor antagonists or agonists, lipoprotein lipase activators, niacin, and phenytoin and lecin : cholesterol acyltransferase activators, etc. This review aimed to the progress of drugs for regulating high-density lipoprotein and their mechanism, in view of clinical and preclinical aspects.

A study on the association between pregnancy-induced hypertension and mutations for lipoprotein lipase gene / 대한산부인과학회잡지

OBJECTIVE: In the pathogenesis of pre-eclampsia, endothelial cell activation or dysfunction is the central theme and marked dyslipidemia may contribute to endothelial cell dysfunction. The objective of this study was to evaluate the association between pre-eclampsia and the Asp9Asn mutation and the -93G promotor mutation. STUDY DESIGN: DNA was extracted from whole blood or cheek swabs of 224 pre-eclamptic patients, 265 controls, and 106 babies from pre-eclamptic patients. Controls consisted of women who had undergone at least two term pregnancies unaffected by pre-eclampsia. All samples were genotyped for all the polymorphisms using Polymerase Chain Reaction (PCR) of known allelic variants. Sequences were confirmed on an Applied Biosystems 373 DNA Sequencer. Results were analyzed with a x2 contingency table. RESULTS: The prevalences of the LPL Asp9Asn mutation and the LPL -93G promotor mutation were not significantly different between the patients with pre-eclamptic patients, severe pre-eclamptic patients, and HELLP syndrome patients and controls and also not significantly different between the babies born from pre-eclamptic mothers and controls. CONCLUSION: In this caucasian population, the LPL Asp9Asn mutation and the LPL -93G promotor mutation are not associated with an increased risk for pre-eclampsia and HELLP syndrome.

Association of Lipoprotein Lipase Gene Polymorphisms with Plasma Lipids in Ischemic Stroke Patients

BACKGROUND: Lipoprotein lipase (LPL) is a key enzyme in the processing of triglycerides and plays a central role in lipid metabolism. It has been reported that the polymorphisms in the LPL gene were associated with plasma concentra-tions of HDL cholesterol (HDL) and triglycerides (TG) in coronary heart disease. We evaluated the correlation between the LPL gene polymorphisms and blood lipids in ischemic stroke patients. METHODS: Ninety-six ischemic stroke patients and 88 controls were included in the study. We evaluated polymorphic sites in the LPL gene using the HindIII for the intron 8 and PvuII for the intron 6 to the polymerase chain reaction products in each group. Allele frequencies, polymorphism information contents (PIC), heterozygosity indices of HindIII and PvuII polymorphisms were calculated in each group. Correlations of total cholesterol, HDL cholesterol, TG, and LDL cholesterol levels in the serum with the HindIII and PvuII polymorphisms of the LPL gene were analyzed in each group. RESULTS: The H+ frequencies, 0.786 and 0.752, in the stroke and control groups respectively. The P+ frequencies were 0.623 and 0.710 in stroke and control groups respectively. No significant difference was observed in triglyceride, total cholesterol, HDL, and LDL. CONCLUSIONS: These findings suggest that the HindIII and PvuII polymorphisms in LPL gene may not be associated with the occurrence of ischemic stroke.

Incidence of Lipoprotein Lipase Gene Polymorphism and Correlation with Severity of Coronary Artery Disease in Korean

BACKGROUND: Lipoprotein lipase(LPL) is a key enzyme in the metabolism of serum triglyceride(TG) which is utilized in the peripheral tissue as free fatty acid and stored in adipose tissue. LPL gene consists of 10 exons which encode 475 amino acids and more than 9 LPL gene polymorphisms have been reported. LPL gene polymorphism is related to lipids level and the severity of atherosclerosis in coronary artery disease. In Korea, LPL polymorphism has not been reported yet. The purpose of this study is to konw the incidences of LPL gene polymorphism and it's relationship with blood lipids level and the severity of atherosclerosis. METHODS: Subjects were divided into three groups; normal controls(n=50), coronary artery disease(CAD, n=51) and cerebrovascular disease(CVD, n=52). The PCR- amplified genomic DNA from peripheral white blood cell was analyzed with restriction fragment length polymorphism(RFLP) by two different restriction enzymes(Pvu II, Hind III). RESULTS: Total cholesterol(TC) was higher in CVD than in controls and CAD (203+/-60mg/dl vs 188+/-37, 167+/-42, p<0.01). Triglyceride(TG) was also elevated in CAD(166+/-65mg/dl vs 122+/-62 in controls, p<0.05). HDL cholesterol(HDL-C) was higher in controls than in CVD and CAD(49+/-9mg/dl vs 36+/-10, 44+/-9, p<0.05). The incidence of Hind III RFLP and Pvu II RFLP was not different among groups. There was no correlation between LPL gene RFLP and lipid profile. There was no correlation between LPL gene RFLP and severity of coronary arterial stenosis. The incidence of Hind III RFLP (-/-) homozygotes was lower in Korean than in other country(5% vs 7-10%). The incidence of Pvu II RFLP (-/-) homozygotes was lower in Korean than in other country(10.3% vs 18-29%). CONCLUSIONS: The LPL gene mutations in intron 6 and 8 have no direct effects on the lipid profiles and the severity of coronary artery disease. Although LPL is a key enzyme in TG metabolism, two mutations in this study could not change the activity of LPL, nor were a marker linked to other site of mutation(s). The mutation(s) in exon which encode amino acid for enzyme activity should be detected to dissect the pathphysiologic mechanism in the atherogenesis.

Polymorphisms at the Lipoprotein Lipase Gene: Possible Associations with Coronary Artery Disease and Blood Lipid Levels in Koreans / 대한임상병리학회지

BACKGROUND: Lipoprotein lipase (LPL) gene polymorphisms have been found associated with coronary artery disease (CAD) and lipid levels, but their impact is less clearly established. The analysis of associations of LPL gene polymorphisms with CAD and lipid levels in Koreans was investigated. METHODS: Analysis of PvuII (intron 6), HindIII (intron 8), and Ser447-Ter (exon 9) polymorphisms of LPL gene were performed using restriction enzyme digestion of amplified DNA products and lipid levels were analyzed in healthy control subjects (n=228) and patients with CAD (n=166). RESULTS: PvuII, HindIII, and Ser447-Ter sites were in strong linkage disequilibrium. No statistical differences in the genotypic frequencies of PvuII, HindIII, and Ser447-Ter polymorphisms were observed between control and CAD groups. P2P2 genotype had higher triglyceride level in CAD group and lower HDL-cholesterol level in control group than the other genotypes (P1P1, P1P2). H2H2 genotype had higher triglyceride level in CAD group and lower HDL-cholesterol level in control group than the other genotypes (H1H1, H1H2). CONCLUSIONS: Genotypes of LPL PvuII, HindIII, and Ser447-Ter polymorphisms were not associated with CAD. Individuals with P2P2 and H2H2 genotypes, however, had higher triglyceride and lower HDL-cholesterol levels that is known to be the most commmon dyslipidaemia in CAD patients.

STUDY ON THE SENSIBILITY OF LPL-PVUⅡ GENE POLYMORPHISM TO INTERVENTION OF COARSE GRAIN FOOD IN DYSLIPIDEMIA POPULATION / 营养学报

Objective: To explore the different genotypes’ susceptibility to intervention of coarse grain food in dyslipdemia population. Method: 409 middle and old aged residents with blood lipoprotein abnormalities were randomized to 2 groups: the intervention group (230 subjects) and the control group (179 subjects). Everyone of the former group was given a steamed bread of coarse grain food per day and health education once every month; while the control group was only given health education. Intervention period was 6 months. The genotypes of LPL-Pvu Ⅱ of the intervention group were analyzed by PCR-RFLP (restriction fragment length polymorphism) technique. Results: All indices in intervention group except WHR were improved after intervention. BMI, WHR, SBP, DBP, serum TG were significantly lower than control group; while serum HDL-C was significantly higher than control group after intervention. Among all genotype groups, the P+/P+ group was more sensible for the change of serum TG and more significant in the effect of improving blood lipids than the P-/P- group; and the susceptibility of change for TC and HDL-C in serum was least in the P-/P- group. Conclusion: There are good intervention effects of coarse grain food in dyslipdemia population. The susceptibilities to improve blood lipids vary with different geno-types of LPL-Pvu Ⅱ , especially for change of serum TG ,and P+/P+ group was more sensible.

ANALYSIS OF THE RELATIONSHIP BETWEEN LIPOPROTEIN LIPASE GENE POLYMORPHISM AND BLOOD FAT TRAITS / 营养学报

Objective To investigate the effect of diet and gene on blood-fat trait of the individual.Method One hundred and twenty mice were fed with high fat emulsion for 4 w,then the genotypes of LPL were analyzed by PCR-SSCP to investigate their effect on blood-fat traits and some organ performance of mice.Result After fed with high fat emulsion for 4 w,98 mice suffered from hyperlipidemia,while 22 mice did not.And a G/A mutation was found in 220 targeted fragments we amplified at 14355 site,and association analysis showed that the mice with BB genotype had higher TC、TG、LDL、H/W、L/W level than those of the mice with AA genotype(P