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Natural Product Sciences ; : 241-246, 2018.
Article Dans Anglais | WPRIM | ID: wpr-741635

Résumé

The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of H₂O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).


Sujets)
Acide acétique , Chromatographie en phase liquide à haute performance , Ecdysone , Ecdystérone , Hétérosides , Lamiaceae , Méthodes , Phénol , Plantes , Rutoside
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