Evaluating the quality of Miao medicine Periploca forrestii Schltr based on 6 caffeoylquinic acid components combined with stoichiometry / 中草药

Objective: To investigate the effects of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C in effective parts of rhizoma arthritis against rhizoma arthritis from different sources of Miao medicine Periploca forrestii Schltr. The determination of 6 kinds of caffeoacylquinic acid chemical components and comprehensive analysis of their quality combined with chemometrics provide a basis for a reasonable and effective evaluation of the efficacy of Miao Medicine Periploca forrestii Schltr against rheumatoid arthritis. Methods: Simultaneous determination was performed by HPLC-UV method. The column was Xtimate C18 (250 mm×4.6 mm, 5 μm), the mobile phase was 0.1% formic acid-acetonitrile, gradient elution, the injection volume was 10 μL, and the flow rate was 1 mL/min. The column temperature is 25 ℃ and the detection wavelength is 327 nm. SPSS24.0 and SIMCA14.1 software are used for comprehensive analysis and evaluation of the quality of medicinal materials. Results: The content of 21 batches of Periploca forrestii Schltr samples from different sources was determined. Through the cluster analysis, the 21 batches of samples were classified into type III, of which the quality of types II and III was better; principal component analysis and partial least squares discriminant analysis The results were consistent with the clustering results. The chlorogenic acid and isochlorogenic acid C were screened to be the markers that caused the differences in the quality of Periploca forrestii Schltr from different sources. Conclusion The determination of the contents of six caffeoacylquinic acid components combined with stoichiometry can further evaluate the efficacy and quality of the anti-rheumatoid arthritis effect of Periploca forrestii Schltr, and provide a reference basis for the quality control of Periploca forrestii Schltr.

Chemical constituents of Shenkun Yangxue Granules based on UPLC-Q-Orbitrap HRMS / 中草药

Objective To study the chemical compositions of Shenkun Yangxue Granules. A novel ultra high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) was established to rapidly and accurately recognize the complex chemical constituents of Chinese materia medica. Methods The information of accurate mass in Full-MS and multistage fragment ions was obtained by UPLC-Q-Orbitrap HRMS technology. Chemical constituents were identified by comparing their relative retention times and the mass data with those of reference substances, as well as consulting the reference literature or Chemical Book Network Database. Results Fifty-one compounds were finally identified from Shenkun Yangxue Granules, including the organic acids, flavones, quinones, glycosides, and others. Conclusion The method established in this study can identify various chemical constituents of Shenkun Yangxue Granules rapidly, accurately and systematically. What’s more, our research will lay a sound theoretical foundation and scientific study idea for improving the quality control, bioactive components recognition and further clinical application of this herbal formulation.