Résumé
Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22(N), 23, 25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (1) and 26-O-β-D-glucopyranosyl-(25R)-5α-furost-20(22)-en-3β, 26-diol (2), together with 7 known ones including 26-O-β-D-glucopyranosyl-(25R)-5, 20(22)-dien-furost-3β, 26-diol (3), (25R)-5-en-spirost-3β-ol-O-β-D-glucopyranosyl-(1→4)-[α-L-rhmanopyranosyl-(1→2)]-β-D-galactopyranoside (4), funkioside D (5), aspidistrin (6), tigogenin-3-O-β-D-lucotrioside (7), desglucolanatigonin II (8), and degalactotigonin (9), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides (6) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.
Sujets)
Humains , Alcaloïdes , Chimie , Toxicité , Antinéoplasiques , Chimie , Toxicité , Lignée cellulaire tumorale , Survie cellulaire , Hétérosides , Chimie , Pharmacologie , Toxicité , Concentration inhibitrice 50 , Structure moléculaire , Phytostérols , Chimie , Toxicité , Extraits de plantes , Chimie , Toxicité , Plantes médicinales , Chimie , Solanum , Chimie , Stérols , Chimie , Pharmacologie , ToxicitéRésumé
Two previously undescribed steroidal compounds, 16, 23-epoxy-22, 26-epimino-cholest-22(N), 23, 25(26)-trien-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside (1) and 26-O-β-D-glucopyranosyl-(25R)-5α-furost-20(22)-en-3β, 26-diol (2), together with 7 known ones including 26-O-β-D-glucopyranosyl-(25R)-5, 20(22)-dien-furost-3β, 26-diol (3), (25R)-5-en-spirost-3β-ol-O-β-D-glucopyranosyl-(1→4)-[α-L-rhmanopyranosyl-(1→2)]-β-D-galactopyranoside (4), funkioside D (5), aspidistrin (6), tigogenin-3-O-β-D-lucotrioside (7), desglucolanatigonin II (8), and degalactotigonin (9), were isolated from Solanum lyratum Thunb. Their cytotoxic activities were tested in two cancer cell lines by MTT method. One of the steroidal glycosides (6) showed significant cytotoxic activity against gastric cancer SGC7901 and liver cancer BEL-7402 cells.
Sujets)
Humains , Alcaloïdes , Chimie , Toxicité , Antinéoplasiques , Chimie , Toxicité , Lignée cellulaire tumorale , Survie cellulaire , Hétérosides , Chimie , Pharmacologie , Toxicité , Concentration inhibitrice 50 , Structure moléculaire , Phytostérols , Chimie , Toxicité , Extraits de plantes , Chimie , Toxicité , Plantes médicinales , Chimie , Solanum , Chimie , Stérols , Chimie , Pharmacologie , ToxicitéRésumé
Objective: To establish the introduction and culture system of the hairy roots in Solanum lyratum and to screen the clone of hairy roots with more diosgenin. Methods: The explants of S. lyratum were infected by Agrobacterium tumefaciens strain C58C1, to obtain the hairy roots and construct the genetic transformation system of the hairy roots in S. lyratum. HPLC was used to determine the diosgenin in the hairy roots. Results: The optimum transformation results were obtained with the max inductivity of hairy roots of 83.33% during infecting time for 10 min by C58C1 and co-cultural time of 4 d. The average content of diosgenin in the hairy roots was 4.620 mg/g, it was 2.652 times as high as that in the leaves (1.742 mg/g) which had the highest diosgenin content in the different tissues of wild type plant of S. lyratum. Conclusion: It is an effective way to obtain diosgenin from the hairy roots of S. lyratum.
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OBJECTIVE:To study the chemical compositions of n-butabol extract from Solanum lyratum. METHODS:Glucan LH-20 column chromatography,silica gel column chromatography and TLC were adopted to separate and purity the chemical com-positions,physicochemical property and spectral evidence to identify their structures. RESULTS:Totally 10 chemical compositions were separated from n-butabol extract,namely apigenin-7-O-β-D-apiofuanosyl(1→2)-β-D-glucose (1),apigenin-7-O-β-D-glucose (2),adenosine(3),3-methoxy-4-hydroxy-5-[(8′S)-3′-methoxy-4′-hydroxyl-phenyl-alcohol]-E-cinnamic-phenylpropyl alcohol-4-O-β-D-glucoside (4),N-(4-amino-butyl)-3-(3-hydroxy-4-methoxy-phenyl)-E-acrylamide (5),N-(4-amino-butyl)-3-(3-hydroxy-4-me-thoxy-phenyl)-Z-acrylamide (6),resveratrol (7),naringenin (8),quercetin (9) and dioscin (10). CONCLUSIONS:Compound 1-8 are first separated from S. lyratum,the study can lay a foundation for quality evaluation of S. Lyratum.
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Objective:To study the anti-inflammatory activity of total alkaloids from Solanum lyratum.Methods:Human umbili-cal vein endothelial cells ( HUVECs) were cultured and induced by H 2 O2 (200 μmol· L-1 ) , and RAW 264.7 cells were cultured and induced by lipopolysaccharide ( LPS ) .The two inflammatory cell models were randomly divided into the normal group , model group, positive control group, and total alkaloids group respectively at low , medium and high dose.After the treatment, the cells were continued to be cultured , and CCK-8 method was applied to observe the cell survival rate .SD rats were randomly divided into the nor-mal group, model group, positive control group, total alkaloid group respectively at low, medium and high dose,and then the rats re-ceived subplantar injection of carrageenan in the paw .After the treatment , inflammation was analyzed by the swelling degree of acute ankle joint injury, and the contents of prostaglandin E2 (PGE2) and cyclooxygenase-2 (cox2-) were detected.Results:The effect of total alkaloids from Solanum lyratum at medium dose on H 2O 2-induced HUVECs and that of total alkaloids from Sola num lyratum at high dose on LPS-stimulated macrophages were similar to that of the positive control group without statistical significance (P>0.05), and the total alkaloids from Solanum lyratum at medium and high dose could significantly reduce the swelling degree of the acute ankle model in rats (P<0.01), and decrease the content of PGE2 in the toe exudate of rats and that of COX-2 in serum (P<0.01) with statistical significance when compared with that in the model group (P<0.01).Conclusion:The total alkaloids from Solanum lyra-tum have significant anti-inflammatory activity , and it is necessary to further study the efficacy and action mechanisms .
Résumé
OBJECTIVE: To study the chemical components of the aqueous extract of Solanum lyratum. METHODS: The compounds were isolated and purified by column chromatography and their structures were elucidated by spectroscopic methods. RESULTS: Nine phenylpropanoids were obtained from aqueous extracts of Solanum lyratum. The structures were determined as chlorogenic acid(1), crptochlorogenic acid(2), neochlorogenic acid(3), erythro-1,2-bis-(4-hydroxy-3-methoxyphenyl)-1,3-propanediol(4), threo-1,2-bis-(4-hydroxy-3-methoxyphenyl)-1,3-propanediol(5), erythro-1,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol (6), threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-methoxypropanol (7), syringaresinol(8), and liriodendrin(9). CONCLUSION: Compounds 2-9 are isolated from Solanum lyratum for the first time.
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The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb. The animals were allocated to two groups, and were administered chlorogenic acid or extract of S. lyratum Thunb. at a dose of 50.0 mg/kg orally. Blood samples were collected up to 8 h post-dosing. Plasma chlorogenic acid analyses were performed using an HPLC method with UV detector. The pharmacokinetic parameters were evaluated using non-compartmental assessment. Significant differences existed in the two groups for AUC0-t, AUC0-∞ and CLz/F. The reliable HPLC method was successfully applied to the determination of chlorogenic acid in rat plasma at dosage of 50.0 mg/kg.
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A rapid method for the simultaneous determination of daidzein, genistein and formonetin in Solanum Lyratum Thunb by high performance liquid chromatography (HPLC) was developed. Separation was achieved on a Diamonsil C18 column (250 mm X 4.6 mm, 5 μm) with isocratic elution, using a mobile phase of methanol-tetrahydrofuran-water (44:3:53, v/v). The wavelength was set at 260 nm and column was maintained at 35°C. The linear ranges of daidzein, genistein and formonetin were 1.0-40.0, 0.1-4.0 and 0.1-4.0 μg/mL, respectively. The average recoveries were between 98.4% and 101.3 %. This method could be used for the quality control of Solanum lyratum Thunb due to its simplification, reliability, rapidity and excellent precision.
Résumé
Objective To investigate the chemical constituents of the 60% alcohol extract of Solanum lyratum Thunb..Method The compounds were isolated by column chromatography over silica gel and Sephadex LH-20 and preparative TLC.Their structures were elucidated on the basis of physicochemical property and spectral data.Resulut Eleven compounds were isolated and identified as:ononin(1), genistin(2), 5-hydroxyl ononin(3), formononetin(4), daidzein(5), daidzin(6), 4-hydroxy-benzaldehyde(7),vanillic acid(8), protocatechuic acid(9),ethyl-α-D-arabinofuranoside(10) and ursolic acid(11).Conclusion Compounds 1,2,3,10 and 11 are isolated from S.lyratum for the first time.
Résumé
A rapid method for the simultaneous determination of daidzein, genistein and formonetin in solanum Lyratum Thunb by high performance liquid chromatography (HPLC) was developed. Separation was achieved on a Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with isocratic elution, using a mobile phase of methanol-tetrahydrofuran-water (44∶3∶53, v/v). The wavelength was set at 260 nm and column was maintained at 35 ℃. The linear ranges of daidzein, genistein and formonetin were 1.0-40.0, 0.1-4.0 and 0.1-4.0 μg/mL, respectively. The average recoveries were between 98.4% and 101.3%. This method could be used for the quality control of Solanum lyratum Thunb due to its simplification, reliability, rapidity and excellent precision.
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OBJECTIVE: To explore the effect of ethanol extracts of Solanum lyratum on apoptosis of SGC-7901 cells and the mRNA expression of apoptosis associated genes Fas and caspase-3.METHODS:SGC-7901 Cells were cultured in vitro and randomly divided into the contral group,ethanol extracts of S.lyratum treatment groups(2.5 mg?mL-1,5 mg?mL-1,10 mg?mL-1) and cisplatin group.The proliferation inhibitory rate was evaluated by MTT assay.Morphological changes and apoptosis of SGC7901 cells were observed under fluorescence microscope.The mRNA expression of Fas gene and caspase-3 were detected by semi-quantitive RT-PCR method.RESULTS: As compared with control group,the cell proliferation inhibitory rate and apoptosis rate of SGC-7901 cells were increased obviously in S.lyratum treatment groups(P
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OBJECTIVE:To explore the effects of ethanol extracts of Solanum lyratum Thunb(ST) on induction of apoptosis and the expression of apoptosis associated genes fas and caspase-3 in human lung cancer SPC-A-1 cells.METHODS:Cultured human lung cancer SPC-A-1 cells were randomly divided into the control group,ethanol extracts of ST treated groups(2.5、5、10 mg?L-1)and the positive control group(cisplatin).After treatment with drug for 48 h,the proliferation inhibitory rate was evaluated by MTT assay,induction of cell apoptosis rate was determined by TUNEL method,the expression of fas and caspase-3 mRNA was detected by semi-quantitive RT-PCR.RESULTS:Compared with control group,the inhibitory rate was increased obviously(P