Résumé
Objective To investigate the preventive effect of nabumetone on the damaged esophageal mucosa in the model of reflux esophagitis, and the effect of expression of cyclooxygenase-2(COX-2), proliferating cell nuclear antigen(PCNA), cyclin D_ 1 (CD_ 1 ) on the progress of reflux esophagitis. Methods A total of 100 SD rats were divided into three groups in random: group Y: operation + saline as positive controls(46); group R: operation + nabumetone(46); and group C: normal control(8). The lesions of esophageal mucosa were observed in the 5th, 17th, 28th , 40th week in groups Y and R and 40th week in group C, respectively. The change of COX-2, PCNA, and CD_ 1 by immunoperoxidase staining in the progress of reflux esophagitis(RE) and the effect of nabumetone were evaluated. Results The lesions of esophageal mucosa at different time and the incidence of Barrett's esophagus(BE) in group R were lower than those in group Y (P
Résumé
Objective To investigate the expression of COX-2,PCNA and Cyclin D_1 in the progress of reflux esophagitis.Methods A total of 54 SD rats were divided into two groups randomly model group were treated with esophagoduodenostomy;control group were normals.The lesions of esophageal mucosa were observed in the 5~(th),17~(th),28~(th),40~(th) week in model group and 40~(th) week in control group,respectively.The changes of COX-2,PCNA and Cyclin D_1 were evaluated by immunoperoxidase staining in the progress of reflux esophagitis.Results The positive rates of COX-2,PCNA and Cyclin D_1 were 0.0%,0.0% and 12.5% in normal respectively,and were(42.1%),42.1% and 42.1% in RE respectively,73.7%,63.2% and 63.2% in BE,and 100.0%,100.0% and 100.0% in EAC,respectively.The expression of COX-2,PCNA and Cyclin D_(1) increased gradually with the development of reflux esophagitis,and significant difference was found between RE,BE and EAC,no difference was found between BE and EAC.Conclusion In the progress of RE,COX-2,PCNA and Cyclin D_1 increased gradually.