Résumé
Saliva is a biological fluid with multifactorial functional applications with regards to themaintenance of general health. Collection of saliva is non-invasive, easy and cost-effective. Xerostomia, a subjective sensation of dry mouth is one of the most common complaints in elderly and patients on polypharmacy as it is proven as a risk factor. Polypharmacy is the simultaneous use of multiple drugs by a patient for more than one systemic disease. Theaim of the study was to assess the salivary changes in patients on polypharmacy. Unstimulated saliva samples were collected from 20 individuals who reported to the Department of Special Care Dentistry at Saveetha Dental College between November to December 2019.The collected samples were then processed for analysis of three salivary parameters-Salivary glucose, amylase and pH. The data were recorded and statistically analyzed. Out of the 20 samples,12 male (60%) and 8 female(40%) with mean age group as 41.2 years. Independent t test showed statistically significant (p<0.05) values for salivary parameters in patients on polypharmacy medication. This study has shown a significant increase in salivary parameters and further studies relating the salivary changes, systemic disease and the medication involved salivary analysis can be suggested as one of the investigations in patients on polypharmacy.
Résumé
To observe the effects of Atractylodis Macrocephale Rhizoma processed by different methods (sulfur-fumigation, different temperatures baking and microwave sterilization) on salivary amylase and D-xylose excretion rate in spleen deficiency rats. The rats were divided into blank control group, rhubarb-induced spleen deficiency model control group, and Atractylodis Macrocephale Rhizoma experimental groups processed with different methods. Amylase colorimetric method was used to determine the activities of salivary amylase and D-xylose excretion rate was measured with O-benzylamine method. Then the correlation of salivary amylase activity and D-xylose excretion rate in urinary was analyzed. As compared with blank control group, Atractylodis Macrocephale Rhizoma baked at 100,110 ℃ can increase the unit content of rat salivary amylase and D-xylose excretion rate, with a significant difference (P<0.05). As compared with the model group, Atractylodis Macrocephale Rhizoma baked at 70 ℃ and Atractylodis Macrocephale Rhizoma with microwave treatment had stronger effects than the others, with statistically significant differences (P<0.01). Atractylodis Macrocephale Rhizoma could improve D-xylose absorption function and salivary amylase activity in spleen deficiency rats. In addition, D-xylose excretion rate in urine was positively correlated with salivary amylase activity. Atractylodis Macrocephale Rhizoma processed with different temperatures baking and microwave sterilization had little impact on salivary amylase activity and D-xylose excretion rate in urine of spleen deficiency rats, while sulfur fumigation had great effects on the above two indexes.
Résumé
Background: Diabetes mellitus is a complex multisystem metabolic disorder characterized by a deficit in the production of insulin. The oral complications of uncontrolled diabetes mellitus are devastating. Saliva is an organic fluid that can be collected noninvasively and by individuals with limited training. These reasons create an interest in evaluating the possibility of using saliva as a diagnostic tool. Aims and Objectives: The aim of this study was to determine, if saliva can be used as a noninvasive tool to monitor glycemic control in Type 2 diabetes. Comparative assessment of salivary (glucose, amylase, total protein levels) in patients with Type 2 diabetes and controls. Materials and Methods: A total of 40 individuals, 20 with Type 2 diabetes and 20 controls of age group 40–60 years were selected for the study. Diabetic status was assessed by estimating random blood glucose levels. Unstimulated saliva was collected from each participant and investigated for glucose, amylase, and total protein levels. Salivary glucose estimation was performed using glucose‑oxidase method, amylase by the direct substrate kinetic enzymatic method, and total protein by pyrogallol red dye end point method. All the parameters were subjected to statistical analysis using SPSS version 20.0. Results: Significantly higher salivary glucose, lower amylase, and total proteins were observed in patients with Type 2 diabetes than controls. There was no significant correlation between salivary and blood glucose levels. Conclusion: These results suggest that diabetes influences the composition of saliva. Since a significant correlation was not observed between salivary and blood glucose levels, further research is needed to determine salivary glucose estimation as a diagnostic tool for diabetes mellitus.
Résumé
Focused relaxation fosters mental and physical repose using stretching and breathing techniques in a pleasant environment. In this study, we analyzed the usefulness of this relaxation technique at the workplace by examining autonomic nervous system activity and psychological effects. Eleven healthy adults (7 males and 4 females) participated in this relaxation technique at a workplace. We investigated heart rate variability, salivary amylase levels and psychological state (Profile of Mood States) before and after the relaxation technique. Average levels of salivary amylase declined from 29.2±12.7kIU/L (mean±standard deviation) to 23.2±10.9kIU/L during the relaxation technique (p=0.05). Average heart rate was also reduced from 90.8±10.0beats/min to 84.9+8.9beats/min during the relaxation technique (p<0.01). Average hig-frequency band power values increased from 315.1±211.3msec2 to 381.8±225.3msec2 during the relaxation technique (p=0.02). Tension Anxiety score of Profile of Mood States decreased from 40.5±4.6 to 35.8±3.3 after the relaxation technique (p<0.01), and the Fatigue score of Profile of Mood States declined from 43.8±6.2 to 40.4±4.1 after the relaxation technique (p<0.05). These results suggested that the relaxation technique increased parasympathetic nervous system activity and decreased sympathetic nervous system activity. This relaxation technique can easily be performed at the workplace without special equipment and can elicit a subjective improvement in fatigue and anxiety in workers.
Résumé
Focused relaxation fosters mental and physical repose using stretching and breathing techniques in a pleasant environment. In this study, we analyzed the usefulness of this relaxation technique at the workplace by examining autonomic nervous system activity and psychological effects. Eleven healthy adults (7 males and 4 females) participated in this relaxation technique at a workplace. We investigated heart rate variability, salivary amylase levels and psychological state (Profile of Mood States) before and after the relaxation technique. Average levels of salivary amylase declined from 29.2±12.7kIU/L (mean±standard deviation) to 23.2±10.9kIU/L during the relaxation technique (p=0.05). Average heart rate was also reduced from 90.8±10.0beats/min to 84.9+8.9beats/min during the relaxation technique (p<0.01). Average hig-frequency band power values increased from 315.1±211.3msec<SUP>2</SUP> to 381.8±225.3msec<SUP>2</SUP> during the relaxation technique (p=0.02). Tension Anxiety score of Profile of Mood States decreased from 40.5±4.6 to 35.8±3.3 after the relaxation technique (p<0.01), and the Fatigue score of Profile of Mood States declined from 43.8±6.2 to 40.4±4.1 after the relaxation technique (p<0.05). These results suggested that the relaxation technique increased parasympathetic nervous system activity and decreased sympathetic nervous system activity. This relaxation technique can easily be performed at the workplace without special equipment and can elicit a subjective improvement in fatigue and anxiety in workers.
Résumé
Background: Leukemia is a fatal disease. The oral manifestations of the leukemias occur early in the course of the disease and these oral features can at times act as a diagnostic indicator. Saliva has been used as a diagnostic aid in a number of systemic diseases. Materials and Methods: In our study, samples of unstimulated saliva of 30 leukemia patients who were not on chemotherapy were collected and analyzed for salivary amylase and total protein. The oral manifestations and radiographic changes (OPG) were recorded. The correlation between the oral manifestations and the salivary components (salivary amylase and total protein) was assessed for prognostic significance. Results: In the present study when the mean values of salivary amylase (1280±754 U/ml) and total protein (647.2±320.7 mg%) were compared with that in control subjects. There was a statistically significant difference for amylase levels (P<.05). On intraoral examination the study subjects showed pallor, gingivitis, gingival enlargement, petechiae, and ecchymosis. On the OPG, the radiographic features included generalized rarefaction of bone (20%), thinning of lamina dura (3.4%), generalized alveolar crest bone resorption (30%), thinning of walls of alveolar crypts (6.7%), besides others, e.g., periapical abscess (10%). Conclusions: The saliva of leukemic patients demonstrated obvious changes in composition. A rise in salivary amylase and total protein levels was evident, with the increase in amylase levels being statistically significant.