Résumé
Objective: To observe the inhibitory effects of matrine, oxymatrine and sophordine on expression of CD91 and CD13 and secretion of TNF-α in macrophage RAW264.7. Methods: Macrophages were cultured in 6-hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0,12. 5,25,50,100,200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by Image-Pro plus system (Media Cybemetic, Inc. , 6.0 edition). Meanwhile, the levels of TNF-α in the culture medium of macrophages were examined by ELISA. Results: Matrine, oxymatrine and sophordine could all inhibit CD91 and CD13 expression in macrophage RAW264.7, and the inhibition varied with the concentrations of matrine, oxymatrine and sophordine; the inhibitory effect of matrine was the most prominent one. Moreover, matrine, oxymatrine and sophordine also inhibited the secretion of TNF-α. Conclusion: Matrine, oxymatrine and sophordine can greatly inhibit expression of CD91 and CD13 and secretion of TNF-α in macrophage RAW264.7.
Résumé
Objective To observe the effects of matrine, oxymatrine and sophordine on activity and TNF-? secretion of macrophage RAW264.7. Methods Macrophage were cultivated in 6 holes board and cultivated 1 day with different concentration matrine, oxymatrine and sophordine. Macrophage activity was measured with MTT and content of TNF-? in collected culture medium was measured with ELISA. Results Macrophage activity and the function of secreting TNF-? was restrained by matrine, oxymatrine and sophordine. The higher concentration of matrine, oxymatrine and sophordine was, the more intense the inhibiting effect was. The effect of matrine was more intense to the function of secreting TNF-? of macrophage. Conclusion Matrine, oxymatrine and sophordine can restrain macrophage activity and the function of secreting TNF-?, the effect of matrine is the most intense.