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1.
Journal of Veterinary Science ; : 379-385, 2004.
Article Dans Anglais | WPRIM | ID: wpr-79775

Résumé

Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibuthyl phthalate (DBP) on ozone were investigated after 32- and 52-wk exposures using hprt mutation assay. Male and female B6C3F1 mice exposed to ozone (0.5 ppm), NNK (1.0 mg/kg), DBP (5,000 ppm), and two or three combinations of these toxicants 6 h per day for 32- and 52-wk showed increases in the frequencies of TG rlymphocytes compared to the control groups. Additive interactions were noted from two combination groups compared to the ozone alone in both sexes of 32- and 52-wk studies. The most common specific mutation type in the hprt genes of test materials-treated male and female mice was transversion with very few transition. The results indicate that such dominant transversion may be responsible for toxicity and combined exposure to ozone, NNK, and DBP induces additive genotoxicities compared to ozone alone.


Sujets)
Animaux , Femelle , Mâle , Souris , Cancérogènes/toxicité , Analyse de mutations d'ADN , Phtalate de dibutyle/toxicité , Association médicamenteuse , Hypoxanthine phosphoribosyltransferase/génétique , Tests de mutagénicité , Mutation/effets des médicaments et des substances chimiques , Nitrosamines/toxicité , Ozone/toxicité , RT-PCR , Lymphocytes T/effets des médicaments et des substances chimiques
2.
Arq. neuropsiquiatr ; 57(4): 907-11, dez. 1999. tab
Article Dans Anglais | LILACS | ID: lil-249286

Résumé

The mutation in the hypoxanthine-guanine phosphoribosyltransfere (HPRT) gene has been determined in two brothers affected with Lesch-Nyhan syndrome. Female members of the family who are at risk for being heterozygous carriers of the HPRT mutation were also studied to determine whether they carry the mutation. DNA sequencing revealed that the boys' mother heterozygous for the mutation in her somatic cells, but that three maternal aunts are not heterozygous. Such carrier information is important for the future pregnancy plans of at-risk females. The mutation, an A_T transversion at cDNA base 590 (590 A_T), results in an amino acid change of glutamic acid to valine at codon 197, and has not been reported previously in a Lesch-Nyhan syndrome male. This mutation is designated HPRT.


Sujets)
Adulte , Adolescent , Humains , Mâle , Femelle , Hypoxanthine phosphoribosyltransferase/génétique , Syndrome de Lesch-Nyhan/diagnostic , Brésil , ADN complémentaire/analyse , , Hétérozygote , Syndrome de Lesch-Nyhan/génétique , Mutation
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