Effect of carbon and nitrogen source amendment on synthetic dyes decolourizing efficiency of white-rot fungus, Phanerochaete chrysosporium.

Decolourization activity of Phanerochaete chrysosporium for three synthetic dyes viz., congo red, malachite green and crystal violet and impact of additional carbon and nitrogen supply on decolourization capacity of fungus were investigated. Maximum decolourizing capacity was observed up to 15 ppm. Addition of urea as nitrogen source and glucose as carbon source significantly enhanced decolourizing capacity (up to 87%) of fungus. In all the cases, both colour and COD were reduced more in non-sterilized treatments as compared to sterilized ones. Significant reductions in COD content of dye solutions (79-84%) were recorded by fungus supplied with additional carbon and nitrogen. A highly significant correlation (r = 0.78, p < 0.001) between colour and COD of dye solutions was recorded. Thus, a readily available carbon and nitrogen source is imperative to enhance the bioremediation activity of this fungus which has been the most suitable for synthetic dyes and textile industry wastewater treatment.

Adsorptive removal of basic dyes from aqueous phase onto activated carbon of used tea leaves: a kinetic and thermodynamic study.

Activated carbon has been prepared from used tea leaves impregnated with H3PO4 (50%, w/v) and carbonized at 300 degrees C. Its adsorption capacity has been tested for the decolourisation of wastewater containing malachite green and methylene blue. The effect of system variables such as concentration, temperature, pH, contact time, adsorbent dosage and particle size was studied. The optimum pH range is 8-10 and dosage required is 1.2 g/L for 100% removal of both malachite green and methylene blue (100 mg/L). Break-through and exchaustive capacities for malachite green and methylene blue are 300.0 and 314.7 mg/g; 275.0 and 290.4 mg/g, respectively. The adsorption data follow Langmuir model as well as Freundlich model.

Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.

A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-mediated destaining procedure, the present method is economical and user-friendly.

Heat mediated quick Coomassie blue protein staining and destaining of SDS-PAGE gels.

For the detection of proteins on sodium dodecyl sulphate-polyacrylamide gel electrophoresis, Coomassie blue is used commonly on account of its simplicity and reliability. In this report we show that enhanced heat, in addition to dramatically decreasing the time required for staining and destaining, also significantly increased the detection sensitivity. For a 1.5 mm gel, the staining time was 5 min at 55, 62.5 or 70 degrees C while the destaining time was 45, 45 and 20 min respectively. For a 0.8 mm gel, the staining time could be reduced to 1 min at 65 degrees C compared to 2 min at 60 degrees C and 5 min at 55 degrees C. The destaining time required was 8, 15 and 20 min at the respective temperatures.

Estudo da diferenciaçäo colorimétrica da dentina cariada em dentes decíduos / A study on the colorimetrical differenciation of carious dentin in primary teeth

Neste estudo foram analisados 40 dentes decíduos cariados de crianças na faixa etária de 2 a 6 anos. Utilizou-se a fucsina básica para identificar a dentina cariada e esta foi classificada em cáries de profundidade média ou profunda e crônicas ou agudas. Numa primeira etapa após a coloraçäo apenas a detina manchada era retirada com curetas de dentina. Como curativo de demora utilizou-se o óxido de zinco e eugenol tipo II por 45 dias. Após este período o dente era aberto e novamente corado e avaliado, comprovando-se a existência de dentina näo contaminada. Pode-se concluir que, é viável aplicaçäo do método proposto por Fusayama em dentes decíduos ou seja, após a segunda coloraçäo da dentina, se esta apresentar cor rosa clara ela poderá se reorganizar