Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 4 de 4
Filtre
Ajouter des filtres








Gamme d'année
1.
Indian J Exp Biol ; 2014 Sept; 52(9): 905-911
Article Dans Anglais | IMSEAR | ID: sea-153775

Résumé

In vitro seedlings were used as explants for protocorm like bodies (PLBs) production which in turn were used for regeneration purpose. PLBs were induced from the base of seedlings (1.0-1.5 cm in size) in MS + BAP (8.88 µM). After 90 days of inoculation, PLBs production rate started declining and most of the PLBs turned into plantlets. Preculture of seedlings in 1.0 µM thidiazuron (TDZ) for 7 days and transfer to BAP supplemented medium resulted in production of 16 PLBs per seedling within 90 days of culture. Increase of TDZ concentration to 2.5 µM and preculture time 15 days, resulted in induction of highest number of PLBs (19 PLBs per seedling) in the basal medium. The results emphasized the importance of thidiazuron (TDZ) concentration and preculture time for PLBs proliferation from the base of seedlings. The PLBs thus produced were used for regeneration studies. Irrespective of single, segmented or clumps of PLBs, the regeneration response was 100% in 2,4-D (4.52 µM) and KN (4.64 µM) but when KN was replaced by BAP (8.88 µM), response was observed only in clumps of PLBs, whereas in single and segmented ones it was 99 and 97%, respectively. Regenerants developed stout root system in half strength M medium supplemented with 2.84 µM of IAA and transferred to greenhouse with 90% survival. The present study holds tremendous potential as the mother plant is not destroyed and PLBs are produced as a continuous system.


Sujets)
Milieux de culture , Germination/effets des médicaments et des substances chimiques , Orchidaceae/effets des médicaments et des substances chimiques , Orchidaceae/croissance et développement , Orchidaceae/physiologie , Régénération , Plant/effets des médicaments et des substances chimiques , Plant/croissance et développement , Plant/physiologie , Techniques de culture de tissus
2.
Biocell ; 36(1): 31-36, Apr. 2012. ilus, tab
Article Dans Anglais | LILACS | ID: lil-657491

Résumé

The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)) and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.


Sujets)
Cryoconservation/méthodes , Déshydratation , Orchidaceae/croissance et développement , Graines/croissance et développement , Cryoprotecteurs/pharmacologie , Orchidaceae/effets des médicaments et des substances chimiques , Régénération , Graines/effets des médicaments et des substances chimiques
3.
Indian J Exp Biol ; 2003 Aug; 41(8): 924-7
Article Dans Anglais | IMSEAR | ID: sea-58761

Résumé

Leaf explants collected from flowering plants of Vanda spathulata were cultured in Mitra medium with combinations of 6-benzyladenine (BA; 13.2-88.8 microM) and indole-3-acetic acid (IAA; 0.0 -85.6 microM). Combination of BA (66.6 microM) and IAA (28.5 microM) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf explants. For optimized multiplication, BA:IAA (2:1 microM) was essential at 22.2- 88.8 microM of BA. Re-cultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 microM of BA and 5.7-28.5 microM of IAA. Rooting of shoots (> 95%) occurred in medium containing banana pulp (75 gl(-1)) and IAA (5.7 microM) within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.


Sujets)
Adénine/analogues et dérivés , Milieux de culture , Acides indolacétiques/pharmacologie , Kinétine , Méristème/effets des médicaments et des substances chimiques , Orchidaceae/effets des médicaments et des substances chimiques , Feuilles de plante/effets des médicaments et des substances chimiques
4.
Indian J Exp Biol ; 2003 Feb; 41(2): 154-9
Article Dans Anglais | IMSEAR | ID: sea-61617

Résumé

In vitro propagation of Anthurium andraeanum Hort. cut flower cultivars viz. Lima White, Tropical White and Tropical Red through organogenesis using mature plant derived leaf explants was established on Murashige and Skoog (MS) medium fortified with different growth regulators. Cultivar, stage and different regions of the source leaf, and type of growth regulators significantly influenced callus induction. Explants from folded brown leaves were superior in induction of callus. Half strength MS medium fortified with 0.88 microM of benzyiadenine (BA), 0.9 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.46 microM of kinetin (Kn) at pH 5.5 was most effective for callus induction. Transfer of callus to medium with 0.54 microM of NAA in place of 2,4-D induced higher number of shoots. Subsequent cultures displayed enhanced rate of shoot initiation and multiplication. Transfer of shoots onto half strength MS medium supplemented with 0.54 microM of NAA favoured rooting of shoots. Cultivar Tropical White was superior in callus, shoot and root induction compared to Lima White and Tropical Red. Plantlets after acclimation in greenhouse were transferred to net-house, that exhibited ninety seven per cent survival. Plants flowered normally between 12 and 15 months and were morphologically similar to that of the mother plants.


Sujets)
Acide 2,4-dichlorophénoxy-acétique/pharmacologie , Adénine/analogues et dérivés , Fleurs/croissance et développement , Herbicides/pharmacologie , Kinétine , Orchidaceae/effets des médicaments et des substances chimiques , Facteur de croissance végétal/pharmacologie , Feuilles de plante/effets des médicaments et des substances chimiques , Racines de plante/effets des médicaments et des substances chimiques , Pousses de plante/effets des médicaments et des substances chimiques , Régénération
SÉLECTION CITATIONS
Détails de la recherche