Evaluation of Widal test in diagnosis of typhoid fever among patients in Aden, Yemen

Salmonella typhi is strictly human pathogen and has no animal reservoir. The laboratory diagnosis of typhoid fever depends upon either clinical investigation or the detection of titers of agglutination of serum antibodies [Widal test]. 216 Yemeni patients with suspected typhoid fever, were investigated with Widal test, slide agglutination test and stool culture. The Widal test showed different results which then compared with conventional broth and solid culture of, MacConkey [MA], Salmonella-Shigella agar [SSA], Bismuth Sulfite Agar [BSA], Xylose Lysine Deoxycholate [XLD] and Kligller Iron Agar [KIA]. The presence of S. typhi was in 4 patients [1.85%], whereas S. paratyphi AB was detected in 24 patients [11.11%]. The stool cultures and biochemical tests the existence of S. typhi the causative agent of typhoid fever. Then the typhoid fever was further confirmed by the API 20E system

Bacteriological analysis of water samples from tsunami hit coastal areas of Kanyakumari district, Tamil Nadu.

Water borne diseases such as cholera, enteric fever and dysentery were expected after the tsunami, which hit the coastal areas of Kanyakumari district, Tamil Nadu. In the present study 151 drinking water sources were collected from the tsunami affected villages and relief shelters and tested for coliforms and pathogens. Nine well water samples were also collected for specific bacteriological analysis. Presence of coliforms was detected in 56 (37%) water samples. One isolate each of Salmonella Paratyphi B and NAG Vibrio were isolated from two well water samples. There was no report of acute diarrhoeal diseases or typhoid illness during the post tsunami period monitored by a field microbiology laboratory for a month.

Survivability of Salmonella paratyphi B var Java on experimentally infected cockroaches.

Cockroaches are inhabitant of sewers and frequent visitors of kitchen and stores in the night to feed on left over. It is liable to disseminate a number of pathogens by contaminating kitchen surface, feeding vessels and food items left open. Salmonella paratyphi B var Java a common pathogen of man and animal was used in the study to evaluate its survival and excretion in cockroaches. The host when fed on semisolid feed containing 1 x 10(7) CFU of S. paratyphi B var Java g-1, it was found that the pathogen was eliminated earlier from the live than euthanized cockroaches.

Fiebre tifoidea con aislamiento de varias especies de salmonella: análisis de tres casos clínicos / Typhoid fever with isolation of several salmonella specimens: 3 clinical cases analysis

En un estudio comparativo t sistemático de los resultados del hemo y coprocultivo hemos podido demostrar la presencia de varias especies de Salmonella en un mismo paciente con el diagnóstico de FT. Es muy difícil sospechar esta infección múltiple si no se hacen estos exámenes para un mejor diagnóstico bacteriológico. Si hay discrepancias entre los hallazgos de sangre y deposiciones, un estudio cuidadoso de las colonias que aparecen en los cultivos permite identificar diferentes especies de Salmonella, con cierta frecuencia. Consideramos que el coprocultivo es un excelente método complementario al hallazgo de la Salmonella en la sangre, significativamente mejorando el diagnóstico bacteriológico de la FT al hacerlos en forma rutinaria. la fagotipificación ayuda, aún más, a esclarecer el problema de las infecciones múltiples por varias especies de Salmonella, ya que hemos podido demostrar fagotipos distintos en un mismo enfermo

Influência da temperatura de incubaçäo no isolamento de salmonelas / Incubation's temperature in the salmonella isolation

No presente trabalho foram utilizadas duas temperaturas de incubaçäo, 37§C e 41§C, para o isolamento de salmonelas, a partir de coproculturas. O maior número de isolamentos se deu na temperatura de 41§C, exceto para a S. typhimurium cujo maior número de isolamentos ocorreu a 37§C.