Résumé
The aim of this study was to investigate the effects of human tryptases on inflammatory cell accumulation in vivo. Various concentrations of purified lung or skin tryptases were injected into the peritoneum of BALB/c mouse. At 6 hours or 16 hours following injection, cells from the peritoneal lavage were collected and stained with modified Wright's stain. Differential cell counts were performed and results were expressed as absolute numbers of each cell type per mouse peritoneum. The results showed a dose-dependent infiltration of neutrophils with a maximal increase of up to 32 fold or 43 fold in numbers at 16 hours following an injection of skin and lung tryptases, respectively. Skin tryptase was able to attract more eosinophils than lung tryptase. Significant increases in lymphocyte and macrophage numbers were also observed. In conclusion, both skin and lung tryptases are able to induce nucleated cell accumulation in the peritoneum of mice with similar specificity and potency.
Sujets)
Agents angiogéniques/administration et posologie , Animaux , Anticoagulants/pharmacologie , Différenciation cellulaire/effets des médicaments et des substances chimiques , Relation dose-effet des médicaments , Héparine/pharmacologie , Médiateurs de l'inflammation/administration et posologie , Injections veineuses , Poumon/enzymologie , Lymphocytes/cytologie , Macrophages/cytologie , Mâle , Souris , Souris de lignée BALB C , Modèles animaux , Infiltration par les neutrophiles/effets des médicaments et des substances chimiques , Granulocytes neutrophiles/cytologie , Péritoine/cytologie , Serine endopeptidases/administration et posologie , Peau/enzymologie , TryptasesRésumé
The effects of hemocoagulase in injectable form (hemocoagulating enzymatic fraction of South American snake Bothrops jararaca venom provided by Ravizza) on the control of intraocular bleeding during vitreous surgery were evaluated in rabbit eyes. Intraocular infusion solution with hemocoagulase (1 NIH thrombin unit/100 ml of BSS plus) significantly reduced the bleeding time. Electroretinogram b-wave and electroretinogram c-wave showed no abnormality. Infusate with hemocoagulase (1 NIH thrombin unit/100 ml of BSS plus) is not toxic to retinal tissue and appeared to be a useful agent for the control of intraocular bleeding during vitreous surgery.