Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 2 de 2
Filtre
Ajouter des filtres








Gamme d'année
1.
Mem. Inst. Oswaldo Cruz ; 109(2): 236-245, abr. 2014. tab, graf
Article Dans Anglais | LILACS | ID: lil-705811

Résumé

Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.


Sujets)
Animaux , Bovins , Techniques de typage bactérien/médecine vétérinaire , Techniques de génotypage/médecine vétérinaire , Mycobacterium bovis/génétique , Tuberculose bovine/génétique , Argentine , Techniques de typage bactérien/méthodes , Bases de données génétiques , Variation génétique , Génotype , Géographie , Techniques de génotypage/tendances , Épidémiologie moléculaire , Réaction de polymérisation en chaine multiplex , Répétitions minisatellites/génétique , Mycobacterium bovis/classification , Tuberculose bovine/transmission
2.
Braz. j. microbiol ; 41(2): 386-390, Apr.-June 2010. ilus
Article Dans Anglais | LILACS | ID: lil-545347

Résumé

Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9 percent). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.


Sujets)
Animaux , Bovins , ADN , Techniques in vitro , Infections à Mycobacterium , Mycobacterium bovis/génétique , Mycobacterium bovis/pathogénicité , Réaction de polymérisation en chaîne , Tuberculose bovine/génétique , Techniques et procédures diagnostiques , Méthodes , Méthodes , Virulence
SÉLECTION CITATIONS
Détails de la recherche