RESUMO
Further investigation of the previously reported in vitro culture of Convolvulus arvensis L. revealed the presence of umbelliferone and scopolet in their identity was confirmed by co-chromatography with authentic samples and their UV data. The composition of the growth regulators, inclusion of yeast extract, sucrose content of the medium and induction of the culture greening by its illumination were found to have a prominent effect on the accumulation level of the coumarins. The greened callus was found to be unable to synthesize neither flavonoids nor alkaloids
Assuntos
Cumarínicos/isolamento & purificação , Extratos VegetaisRESUMO
The induced callus of convolvulus arvensis [L.] was found to have low growth rate. The exogenous growth regulators had a pronounced effect on the callus growth. The cellular composition of the induced callus was found to be normal parenchyma and few vessel segments. Coumarins [umbelliferone and scopolotin] and sterols [mainly B-sitosterol] were found to be the main accumulated secondary metabolites