Comparison between the prospect cryptosporidium microtiter assay and the immunofluorescent test in the diagnosis of cryptosporidiosis

The immunofluorescent test utilizing rabbit antisera against cryptosporidium oocysts isolated from dairy calves was compared with the prospect cryptosporidium microtiter commercial assay, which is an enzyme immunoassay utilizing monospecific antibody to cryptosporidium specific antigen [CSA]. The former test proved to be more sensitive than the latter one in diagnosing human cases positive for cryptosporidiosis by the modified acid fast stain

Sandwich ELISA technique in the detection of circulating immune complexes in schistosoma mansoni infection

Fifty sera of cases infected with S. Mansoni were tested for the detection of circulating immune complexes [CIC] by sandwitch ELISA technique. Ten more sera of healthy volunteers were used as a control. Out of 50 cases 64% were positive whereas 22% were found negative for CIC. Most of the negative cases were having low egg count in the stools. Sandwitch ELISA technique was reproducible and the percent of positivity could be raised if IgE enzyme labeled conjugate is used

Cryptosporidiosis in infants with prolonged diarrhea admitted to the Rehydration Centre, Cairo University

Special attention has been given recently to cryptosporidium, a well known mhteric coccoidian protozoon, vausing diarrhea in man. This study is performed to determine the possible role of this parasite as an aetiologic agent for diarrhea in 130 infants [three to eighteen months of age] attending Abou El Reish Children Hospital Rehydration Centre. Cryptosporidia oocysts were detected in stools of 2[1.53%] female infants

Giardiasis in a rural community in Middle Egypt

Four hundred and forty four individuals were examined parasitologically and clinically in an attempt to assess the role of G. lamblia as a cause of diarrhea in a rural community in Egypt. Bacteriological study was also performed on 51 diarrhea causes during the same period of the study. The prevalence of G. lamblia infection was 19.4% higher in those less than 3 years old. Giardia trophozoites and or cysts were found more in mushy to liquid less uniformed stools. Clinical symptoms due to giardiases recorded in outbreak of diarrhea were common symptoms among our rural community but these symptoms were present in cases whether having Giardia alone, or with other intestinal parasites 18% or in intestinal parasites without Giardia Lamblia 53.3% and even in cases where no intestinal parasites were present 25%. When studying the 51 cases with diarrhea, we found that 7.8% had Giardia alone while 7.8% had G. lambia and other intestinal parasites and 66.7% had bacterial and viral pathogens. Moreover, 19.7% had no aetiologic agent for diarrhea. With respect to the serology, ELISA didn't help in substituting the direct method for diagnosis. Results of the study will be discussed