Detection of interleukin 8 and auto antibodies to interleukin 8 in the serum of patients with lichen planus

An enzyme linked immunosorbant assay ELISA was used to determine the serum concentration of interleukin 8 and auto antibodies to interleukin 8 in fifteen patients with oral lichen planus only, seven patients with oral and skin lichen planus as well as ten healthy control subjects. Interleukin 8 was detectable in the serum of eleven of fifteen patients with lichen planus and five of seven patients with oral and skin lichen planus but none of the healthy controls showed detectable serum interleukin 8. Significantly, elevated auto antibodies to interleukin 8 were detected in two patients groups. However, their values did not correlate significantly with clinical condition of the disease including affected side; distribution and the duration. The results of this work may [lend] support the role of immune mechanism in the etiopathogenesis of oral lichen planus

Clinical and bacteriological evaluation of the periodontal therapy by the placement of acrylic strips containing antibacterial drugs into the periodontal pockets

The role of specific Gram negative bacteria in the etiology and pathogenesis of periodontal disease has been markedly reported in the last two decades. This study evaluates the effect of local application of tetracycline or metronidazole on the subgingival bacterial as well as the clinical condition of the gingiva. Twenty four periodontitis patients were included in this study, each patient had either metronidazole or tetracycline, incorporated into acrylic strips inserted subgingivally once weekly for 3 weeks. Subgingival plaque samples were obtained from each patient immediately prior to insertion and after the removal of the strips. The collected bacterial plaque samples were cultured and identified. The results showed an improved gingival status and pocketing. Bacteriologically, the two drugs peoduced an increased number of cocci species with marked reduction of Gram negative rods, however metronidazole was more effective than tetracycline

Role of T and B lymphocytes in spontaneous lymphocyte proliferation in chronic inflammatory periodontal disease

Spontaneous lymphocyte responses of whole mononuclear cells [MNCs, T-lymphocytes and B-lymphocyte in patients with periodontal disease and theircontrols were studied in this work utilizing the tritiated thymidine uptakeassay. The periodontitis patients exhibited lower lymphoproliferativeresponse of MNCs compared with the controls. However, theB-lymphoproliferative response of patients showed the highest proliferativeresponse compared with that of all cell types studied. This finding maysuggest that the elimination of T-cell affects the hyperresponsiveness ofB-lymphocyte state

Connective tissue response to tetracycline-incorporated resins

This experimental study was undertaken to determine the response of connective tissue to both self-cured and visible light cured resin containing 10% [w: w] tetracycline hydrochloride. The visible light cured resin considered the most compatible material. Marked reduction in the degree of inflammatory cell infiltration in both capsular and pericapsular tissues and increased degree of fibrous elements maturity of the fibrous capsule were noted when tetracycline was added to the test materials, during the whole observable periods

effect of metronidazole on surcutaneous tissues in rats

An attention was paid to evaluate the effect of implants visible light cured resin, and self-cured acrylic resin containing 10% metronidazole, upon the rat connective tissue. The best connective tissue response was obtained when metronidazole was added to self-cured acrylic resin, compared with other tested materials. It can be concluded that some antimicrobial agents can be added beneficially to acrylic resins used in dentistry as means of inflammation reduction, as well as to promote healing

Therapeutic effect of tinidazole in periodontal disease I- clinical evaluation

Twenty-four patients participated in the study. Every patient received scaling and root planing for one side and the other side was left without scaling to serve as control. All patients were given 1 g of tinidazole for 2 days. The gingival index, plaque index, periodontal index and the periodontal pocket depth were recorded before scaling and at 2, 6, 8 weeks. There was an improvement in the periodontal health status, which may suggest the use of tinidazole as valuable adjunct in the periodontal therapy

Autologous mixed lymphocyte reaction in relation to severity of periodontal disease

In this study, spontaneous lymphocyte proliferation was used to detect possible systemic immunoregulatory differences between periodontally affected patients and healthy controls. Sixty subjects were included in this study, they were divided equally into four groups: healthy controls [H], gingivitis patients [G], mid-moderate, periodontitis patients [MP] and severe periodontitis patients. They were periodontally examined, then blood sample was obtained from each participant. Spontaneous proliferation was assessed for each one using peripheral blood mononuclear cells on days 3, 5, 7 and 9 in cultures. The cultures of healthy subject, gingivitis group, moderate periodontitis group and severe periodontitis group were peaked at day 7 culturing. However, the response of all groups showed a rapid decline by day 9 culturing. The findings reported here may suggest a possible role of the immunoregulation in the etiopathogenesis of periodontal disease

Lymphocyte blastogenic response to collagen in periodontal disease is predominantly a B-cell response and dependent upon adherent cells

Peripheral blood lymphocytes patients with periodontal disease undergo blastogenesis with human collagen type I. The cellular characteristics of this response were examined by fractionation of the cell populations and assay of thymidine uptake of responding cells. They were separated on nylon wool columns and monoclonal antibodies [OKT3 and anti-Lew M2] were used to further characterize the cell separation method. B-lymphocytes [OKT3 -] responded at higher levels to collagen than T-lymphocytes [OKT3 +]. Depletion of polystyrene adherent cells reduced the response to a greater extent. Reconstitution with approximately 10% adherent cells gave the maximal lymphoblastogenic response to collagen

Human T-Lymphocyte subpopulations in periapical lesions

Both B-lymphocyte- and T-lymphocyte-mediated immunologic reactions have been implicated in the pathogenesis of human periapical lesions. Thirteen periapical lesions were obtained during periapical surgery, frozen and serially sectioned. Monoclonal antibodies and indirect immunofluorescence techniques were used to compare the distribution of lymphocyte subpopulation in the periapical lesions. The proportions of total T-cells [T11], inducer/helper T-cells [T4+] and cytotoxic suppressor T cells [T8] were determined. No significant statistical difference was found between the number of T helper cells and that of the T cytotoxic cells. The presence of T-cells in periapical lesions indicated that cell-mediated reactions participate in the pathogenesis of these lesions

Impairment of the immunoregulation in rapidly progressive periodontitis

Monoclonal antibodies [MoAbs] were used to characterize the populations and subpopulations of peripheral blood mononuclear cells [MNCs] from 22 patients with rapidly progressive periodontitis and 22 control subjects. The proportion of T-lymphocytes [OKT33] were decreased, but not significant, in the patient's group. However, the ratio of helper/suppressor [OKT4+/OKT8+] was significantly decreased in the patient's group compared with the control group. The percentages of B-lymphocytes [Ig+ cells] and monocytes [anti-leu M33] were markedly increased in the patients with rapidly progressive periodontitis. In the view of these findings, it seems likely that, the changes in proportions of regulatory T-lymphocytes and monocytes may explain, at least in part, the impaired immune response, previously reported to be existed in patients exhibiting sever destruction of periodontal tissues