RESUMO
Introduction: Single nucleotide polymorphisms [SNPs] of the beta[2]-adrenergic receptor [beta[2]-AR] gene have been implicated in the pathogenesis of cardiovascular diseases. This study evaluated two beta[2]-AR SNPs in association with myocardial infarction [MI], namely arginine-glycine [G16R] substitution at codon 16 and glutamine-glutamic [Q27E] substitution at condon 27
Objectives: Therefore, our main objective was to determine the association of these two SNPs among patients with MI with and without type 2 diabetes [T2D]
Materials and Methods: Blood samples were collected from 201 MI patients with and without diabetes and from 115 controls and the beta[2]-AR gene polymorphisms at codon 16 and codon 27 were assessed by restriction fragment length polymorphism. The CHI[2] test was used to compare differences between groups
Results: The SNPs did not deviate significantly from Hardy-Weinberg equilibrium in the control population. The allele and genotype frequencies of the beta[2]-AR gene polymorphism at codon 16 [G16R] was significantly different between MI cases and controls [CHI[2] = 10.495, P < 0.05 and CHI[2] = 8.849, P < 0.05, respectively]. No significant difference in genotype and allele frequencies at codon 27 was shown between these two groups [CHI[2] = 2.661, P >/= 0.05 and CHI[2] = 1.587, P >/= 0.05, respectively]. When the MI patients with and without T2D were pooled together, genotype distribution was different between cases and controls at codon 16 [CHI[2] = 4.631, P = 0.099] and codon 27 [CHI[2] = 7.247, P = 0.027]. However, no significant differences were found in allele frequencies for codon 16 and codon 27 between the two groups [CHI[2] = 0.628, P = 0.428; CHI[2] = 0.33, P = 0.565, respectively]
Conclusion: Our findings indicate a moderate association of the beta[2]-AR G16R gene polymorphism with MI suggesting that this gene plays a universal role in the development of MI across ethnicities. However, there was no association of beta[2]-AR G16R gene polymorphism with diabetic patients with MI