RESUMO
Background: certain specific bacterial species from the subgingival biofilm have demonstrated etiological relevance in the initiation and progression of periodontitis. Among all the bacteria studied, three have shown the highest association with proximal caries and bone loss: Fusobacterium nucleatum [Fn], Capnocytophaga [Ca], and Campylobacter rectus [Cr]. Therefore, the relevance of having accurate microbiological diagnostic techniques for their identification and quantification is clearly justified
Aim: to identify the bacterial pathogens with alveolar bone loss and proximal caries in primary dentition and their association with periodontal disease utilizing deoxyribonucleic acid [DNA] microbial probe testing
Materials and Methods: subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. After extracting DNA, Fn, Ca, Cr, Aggrgatbacter actinomycetemcomitans, Porphyromonas gingivalis and several other periodontopathogens were determined by DNA microbial probe testing method
Results: samples detecting a high bacterial load of Fn, Ca and Cr in children having proximal caries associated with periodontal disease compared to children having proximal caries without periodontal disease [P = 0.01]
Conclusions: results suggested that there was a relationship between microbial pathogens associated with proximal dental caries in the primary dentition and periodontal disease in children. In addition, DNA microbial probe testing technology clearly analyzed the different loads of periodontopathogens in children who had with proximal caries associated with bone loss and is useful in microbial diagnostics for patients in dental practices
RESUMO
Objectives: Transforming growth factor-betas [TGF[beta]s] are multifunctional growth factors with a broad range of biological activities in various cell types in many different tissues. The purpose of this study was to evaluate the treatment of intrabony defects with anorganic bovine bone mineral matrix combined with TGF[beta]-1 with the use of anorganic bovine bone alone
Materials and Methods: Thirty-two sites from sixteen patients were selected using a split-mouth study design for each patient, determined randomly through a biased coin randomization. One site received a mucoperiosteal flap, and the osseous defect was fi lled with the combined therapy [Group 1]. The other site treated was with anorganic bovine bone alone and served as a control [Group 2]. All the treated sites were covered with a bioabsorbable collagen membrane. The clinical parameters and radiographic follow-up examinations were recorded after 3, 6, 9, and 12 months
Results: Clinically, there was a statistically signifi cant gain in the clinical attachment level [+5.03 +/- 0.14 mm] and a statistically signifi cant reduction of pocket probing depth [?5.16 mm +/- 0.13] for Group 1 sites compared to sites in Group 2 [P = 0.01]. In addition, there were signifi cant differences in bone density and a signifi cant decrease of marginal bone loss after the combined therapy compared with the use of anorganic bovine bone alone [P = 0.01]
Conclusions: The use of anorganic bovine bone mineral matrix combined with TGF[beta]-1 seemed to be effective in the treatment of intrabony defects. This showed an improvement in the clinical outcome of periodontal therapy superior to the use of anorganic bovine bone on its own
RESUMO
To assess the frequency and causes of dental anxiety and their relation to irregular dental visits among adult dental patients. The Dental Anxiety Question [DAQ] included within a self-administered questionnaire was distributed to 1025 patients attending the Interns' Dental Clinics in the Department of Preventive Dental Sciences, College of Dentistry, University of Dammam, Dammam, Kingdom of Saudi Arabia from March 2012 to February 2013. A cross-sectional study design was used. The questionnaire consisted of 22 closed-ended questions divided into 4 sections; 1] demographics, 2] regularity of dental visits, and related causes, 3] DAQ, cancellation of dental appointments, history of previous trauma, dental anxiety provoking factors within dental environment and procedures, and 4] patients' status in dental clinics, preferences of dentists, and perceptions regarding dental anxiety. The prevalence of dental anxiety among the study sample was 27%. Anesthetic injection was the main factor of dental fear [88.2%], while dental surgical procedures [35.7%] and extractions [23%] were the most terrifying dental procedures. Lack of time [79.5%], cost [71.5%], far-situated dental services [62.2%], and fear [57.1%] were causes listed for irregular dental visits; while 31.3% had no specific reason. Irregular dental visits were not related to dental anxiety. Dental anxiety continues to be an obstacle despite the vast improvement in dentistry; and this raises an alert regarding personal and communication factors in the patient-dentist relationship. Factors such as equal distribution of dental services, time, and cost should also be addressed