rhIL23R-CHR/Fc fusion protein inhibits Act-HaCaT inflammation and proliferation by downregulating ENST00000522718 / 中国药科大学学报

@#Psoriasis is an autoimmune disease characterized by chronic skin inflammation, and its etiology and pathogenesis have not been fully elucidated to date. In the previous study, rhIL23R-CHR/Fc fusion protein had been found to significantly relieve the symptoms of psoriasis mice and the pharmacological mechanism had been initially elucidated.In this study, we established a psoriasis cell model (Act-HaCaT) using TNF-α-activated human immortalized keratinocytes (HaCat).In our current study, the lncRNA that plays a key role in the regulation of Act-HaCaT function by the rhIL23R-CHR/Fc fusion protein was screened by transcriptome sequencing combined with qRT-PCR.The results showed that rhIL23R-CHR/Fc fusion protein significantly inhibited cell proliferation and inflammatory factor production in Act-HaCaT.lncRNA ENST00000522718 was obtained by screening, and knockdown of ENST00000522718 was found to significantly inhibit cell proliferation and inflammatory factor production.Our findings suggest that ENST00000522718 plays an important role in the pathological mechanism of psoriasis.

Ethical discussion on stem cells research / 中华医学教育探索杂志

Unique totipotency of embryonic stem cell make it great value.Embryonic stem cells have received extensive attention from the discovery,research to application.This paper outlined the discovery of embryonic stem cell and the challenges faced in the progress of the study and research process,focusing on the ethical issues.Meanwhile,this paper analyzed the attitude of various governments,the status of the relevant laws and regulations in order to discuss the prospects of stem cell re

Clinical trial on gatifloxacin against acute exacerbation ofchronic bronchitis (AECB) in adults / 重庆医科大学学报

Objective:To investigate the clinical efficacy and safety of gatifloxacin in the treatment of acute exacerbation of chronic bronchitis(AECB).Methods:23 patients in each group (trial or control) were treated with gatifloxacin or ciprofloxacin,respectively,for 5 to 14 days.Results:The clinical effective rates of the two groups were 91.3% and 78.3%,respectively.The bacterial eradication rates of the two groups were 91.7% and 72.7% respectively.Incidence of adverse reactions of the two groups were 12.5% and 8.7%,respectively.Conclusion:The efficacy and safty of gatifloxacin in treating AECB is reliable and superior to ciprofloxacin.

Genotyping of 238 HBV strains by the methods of type-specific primers PCR / 重庆医科大学学报

Objective:To establish a set of suitable and reliable methods and to study the distribution of HBV genotypes.Methods:Type-specific primers were designed,and type-specific primers PCR was used to genotype the HBV strains.Results:A total of 218 out of the 238 strains were genotyped.Among them,49(68.3%) cases were genotype B(male 69.3%,female 65.3%),63(28.9%) cases were genotype C(male 28.3%,female 30.8%),6(2.8%) cases were mixtures of genotype B and C(male 2.4%,female 3.8%).No genotype A,D,E,F,G and H were found.Conclusion:Genotype B is the most dominant type for HBV strains and there is no relationship between the gender and HBV genotypes.