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Journal of Gynecologic Oncology ; : 328-333, 2014.
Artigo em Inglês | WPRIM | ID: wpr-202217

RESUMO

OBJECTIVE: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. METHODS: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays. RESULTS: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. CONCLUSION: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.


Assuntos
Animais , Feminino , Hormônio Antimülleriano/sangue , Antineoplásicos/toxicidade , Biomarcadores/sangue , Cisplatino/toxicidade , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos/métodos , Preservação da Fertilidade/métodos , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Folículo Ovariano/efeitos dos fármacos , Insuficiência Ovariana Primária/sangue , Ratos Sprague-Dawley
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