Identification of Proteins Associated with the Formation of Oral Biofilms

ABSTRACT Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.

Giómeros fluidos en la eliminación de sensibilidad en molar permanente afectada con Hipomineralización Incisivo Molar (HIM). Reporte de caso / Fluid giomers in the elimination of sensitivity in permanent molar affected by Molar Incisor Hypomineralization (HIM). Case report

RESUMEN: El principal motivo de consulta de los pacientes afectados con hipomineralización incisivo molar grado B 3 II, según la Academia Europea de Odontología Pediátrica (EAPO) es la estética y sensibilidad, provocando dolor a los cambios térmicos y a la hora de realizar el cepillado dental, esto puede conllevar a una rápida progresión de caries dental, por disminuir el cepillado. En el presente caso se describe la reconstrucción y sellado de fosas profundas de la pieza 46 afectada con hipomineralización incisivo molar, mejorando la calidad de vida relacionada a la salud bucal del paciente y eliminando la sensibilidad en una sola cita.

ABSTRACT: The main reason for consultation of patients affected by molar incisor hypomineralization level B 3 II (EAPO) is aesthetics and sensitivity, causing pain with thermal changes, when consuming cold drinks and when brushing the teeth. This can lead to a rapid progression of tooth decay, by decreasing the brushing. In the present case, the reconstruction and sealing of deep pits of the tooth 46 affected by molar incisor hypomineralization are described, improving the quality of life related to the patient's oral health and eliminating sensitivity in a single appointment.

Chemical Composition and Antibacterial Effect of Plantago Major Extract on Periodontal Pathogens

Abstract Objective: To determine the in vitro antibacterial effect of different concentrations of the ethanol extract of Plantago major (plantain) on Porphyromonas gingivalis and Fusobacterium nucleatum. Material and Methods: Bacterial susceptibility tests were used in conjunction with the agar diffusion test and the minimum inhibitory concentration (MIC) test using the broth macrodilution technique. Results: Different concentrations of ethanol extract (25%, 50%, 75% and 100%) dissolved in 70% ethanol were used, with a positive control (0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride) and a negative control (70% alcohol). The extracts at 75% and 100% showed inhibition halos against both strains studied. With 0.12% chlorhexidine + 0.05% cetyl-pyridinium chloride, inhibition halos averaged 14.9 mm, in contrast to 70º alcohol, where no bacterial inhibition was observed. The MIC was 50% for both species. Conclusion: The ethanol extract of Plantago major presents an in vitro antibacterial effect on Porphyromonas gingivalis, they may have potential applications in food and pharmaceutical products.