Susceptibility pattern of streptococcal isolates from oral cavity against commonly used antibiotics

To assess the susceptibility pattern of streptococcal isolates from oral cavity against commonly used antibiotics. Oral samples, including subgingival swabs, were collected from 120 patients aged 12 to 60 years. Two swabs were collected; first for rapid-strep testing for the presumptive diagnosis of Group A Streptococcal infection and second to culture for definitive bacterial identification. Antibiotic sensitivity of the isolated strains of streptococcus was done by direct disk diffusion method. Four different classes of antibiotics; Penicillin, Cephalosporin, Macrolide, and Vancomycin were tested to determine the sensitivity pattern. Out of 100 isolates recovered, the prevalence of streptococcal species was74%. The greatest zone of inhibition was achieved with amoxicillin, amoxicillin-clavulanic acid, and vancomycin [16, 17, and 18 mm respectively]. Zones of inhibition measured for cephalosporins and macrolides were less than the other groups [10.5 and 11 mm respectively]. Amoxicillin is still the drug of choice for prophylactic and therapeutic use in our dental settings not only for its better coverage on the streptococcus species but also due to its easy availability and affordability. This corresponds with the international literature, however, the importance of obtaining specimens for culture and sensitivity should be observed as a standard procedure, to minimize the chances of development of resistance and identify the changing trends of the infectivity mode

Effect of nystatin, gentian violet and garlic extract on candidiasis patients undergoing radiation therapy in head and neck region

To evaluate the in-vivo anticandidal activity of Nystatin, Gentian Violet and Garlic extract on candidiasis patient receiving radiation therapy in Head and Neck region. The in-vivo antifungal activity of Nystatin, Gentian Violet and Garlic Extract were carried out on 60 patients. These patients were divided into 3 groups [Group I = Nystatin, Group 2= Gentian violet, Group 3= Garlic extract]. Each group consisted of 20 patients. The clinical lesion were recorded and graded during the treatment according to the Lindquist/Hickey scale on 0th 7th and 15th day and the anticandidal effect of these drugs were evaluated by oral lesion index evolution through simple mean. Result showed that Nystatin, Gentian violet and Garlic Extract were found to be very effective. It can be concluded from the study that all the drugs were effective against Candida albicans and secondly the organism has not developed any resistance to these drugs

In vitro effect of nystatin, gentian violet and garlic extract on candida albicans isolates from oral cavity

To evaluate in-vitro anticandidal activity of Nystatin. Gentian Violet and Garlic extract on Candida albicans isolates from oral cavity. For screening of antifungal activity of Nystatin. Gentian Violet and Garlic Extract, 100 clinical isolates were collected from the department of Radiotherapy. Jinnah Postgraduate Medical Center and Fatima Jinnah Dental College and Hospital, Karachi. The samples were collected from the oral cavity and the sample site was buccal mucosa, tongue and hard palate. These isolates were finally identified on the basis of morphology and cultural characteristics and confirmed using germ tube method. The antifungal activity of Nystatin, Gentian Violet and Garlic Extract were carried out by disc diffusion method. Result showed that the mean zone of inhibition of Nystatin was 17.9mm, Gentian violet was 16.6mm and Garlic Extract was 16.6mm and all the drugs used were found to be very effective. It can be concluded from the study that all the drugs are very effective against clinical isolates of Candida albicans and secondly the organism had not developed any resistance to these drugs