Association between Interleukin-5 and Body Mass Index among Iraqi asthmatic patients

Asthma is an allergic hypersensitivity disease in which many mediators play a role in its pathogenecity. One of these etiological agents are the mediators such as lnterleukin-5 [IL-5]. The aim of this study is to demonstrate the association between IL-5 and obesity in asthma development. One hundred and four sera samples for asthma cases have been studied in comparison with 41 non-asthmatic bronchitis as patient controls beside 30 apparently healthy controls. Cytokine has been estimated using ELISA method in correlation with Body Mass Index [BMI]. This study revealed a significant correlation between IL-5 concentration and BMI [P<0.05], particularly among females. There is certain correlation between obesity and asthma accompanied by IL-5

Possible Role of lnterleukin-6 in Rheumatoid Arthritis

Rheumatoid Arthritis [RA] is an autoimniune disease. Many etiological agents are proposed to play a role in its pathogenecisy. One of these factors is cytokines such as Interleukin6. ELISA method has been used for IL-6 estimation in 75 RA patients in comparison with 67 SLE as patient controls and 39 apparently healthy controls. This study showed that there was an elevation of IL -6 in the sera of RA patients with high significant differences between RA patients and controls [P < 0.001]. Moreover a good correlation between IL-6 level and RE titer were observed However, for most patients with high IL-6 were shown to be HLA-DR4. Interleukin-6 play a crucial role in the disease which may be participate in the severity of RA and subsequently its treatment

Viral load among the sera of Iraqi hepatitis C virus patients

Hepatitis C Virus is the main causative agent of hepatitis among blood transfused patients, in which most chronic cases result in liver carcinoma. Enzyme-Linked Immunosorbent Assay for HCV, FNF-[] and IL-12p40 estimation with Recombinant Immunoblotting Assay [RIBA] as a confirmatory test for HCV, have been applied for HCV detection in 80 HCV patients' samples in comparison with 30 samples for apparently healthy control, while viral load has been estimated using Branched-DNA [b-DNA] technique for 32 randomly selected positive cases for HCV. Liver function test has been applied for patients' sera in comparison with control. This study reveals highly specificity and sensitivity of ELISA technique for HCV detection which results in 100% positivity by RIBA methods. Moreover, Viral load estimation shows that [71.9%] of HCV sera samples with viral load >615 IU /ml and only [28.1%] with viral load <615 IU/ml with highly significant difference between them [P = 0.013]. Furthermore, there is a highly significant variations between liver function test in comparison with control group [P< 0.01], while SGPT is the only parameter which significantly affected by viral load [P= 0.011]. Beside that, neither INF-[] nor IL-12p40 level has been affected by viral loads more or less 615 IU /ml. It was concluded that ELISA technique is still the best accurate reliable method for viral detection and SGPT is a good marker for highly viral loaded samples

Possible role for lnterleukin-5 in asthma

Asthma is an allergic disease characterized by airway obstruction as a result of cellular accumulation due to the liberation of certain mediators. Among those mediators are the cytokines such as IL-5. lnterleukin-5 concentration has been estimated in 94 sera samples of Asthmatic patients in comparison with 41 non-asthmatic bronchitis as patient controls in addition to 30 apparent healthy control group using ELISA method. There is highly significant elevation of IL-5 in the asthmatic cases in comparison with healthy controls [P< 0.001]. We conclude that this cytokine may play the major role in asthmatic attack and it may be a good marker for the disease