RESUMO
The purpose of this study was to find potential biomarkers in the serum of patients with depression and provide the basis for clinical diagnosis of depression. Sixteen patients of severe depression were selected according to the inclusion criteria and 16 healthy people were used in the control group. The depression patients took paroxetine for two weeks. The serum was collected from the patients and healthy control group before and after paroxetine treatment. The samples were analyzed by 1H NMR based metabolomics to determine the changes in profiles of endogenous metabolites and metabolites with significant differences were selected in analysis. Related pathways and receiver operating characteristic (ROC) were examined in analysis of the correlation between the potential biomarkers and depression. Their feasibility and reliability was determined for the clinical practice. Significant differences were observed in the metabolic profile of serum of the patients and the healthy controls. Depression had an effect on metabolism for an increase in leucine, isoleucine and alanine, glutamate, glutamine and N-acetyl-glycoprotein and a decrease in glucose. Those may be considered as potential biomarkers of depression. Clinical application of the biomarkers may improve the objectivity of the diagnosis and treatment of depression by antidepressant drugs. The metabolomics approach is an effective tool in the investigation of biomarkers.
RESUMO
Objective: To explore the change of endogenous metabolites in serum of rats with erythronoclastic anemia and the intervention of Lvjiao Buxue Granules by using 1H-NMR metabonomics coupled with multivariate statistical analysis. Methods: Acetylphenylhydrazine was used to establish the rat model of erythronoclastic anemia and after a week the rats except control and model groups were given to Lvjiao Buxue Granules (8 g/kg) separately once daily for two weeks. While the rats in the control and model groups were ig given equivalent distilled water respectively. The endogenous metabolites in serum from all of rats were analyzed by 1H-NMR coupled with multivariate statistical analysis. Results: Compared with the control group, the levels of lipids, lactic acid, and acetone in serum of rats in the model group increased while the levels of alanine, valine, creatinine, phosphocholine, glycerophosphocholine, oxidation of three methyl ammonium, glycine, and arginine were decreased. The levels of these endogenous metabolites with significant difference were reversed to normal by ig asminstration of Lvjiao Buxue Granules. Conclusion: The mechanism of Lvjiao Buxue Granules on tonifying blood is involved in the pathway of energy metabolomic, lipid metabolism, and intestinal bacteria metabolism.