RESUMO
<p><b>OBJECTIVE</b>To establish fluorescent quantitative PCR method for detecting human herpes virus type 6 (HHV6).</p><p><b>METHODS</b>According to the specific sequence of human herpes virus type 6 genes, the primers and the fluorescent probe (TaqMan) were designed and synthesized. The fragment generated from HHV 6 gene as template was cloned into the pMD18-T vector which was constructed from the pUC 18. And the positive recombinant plasmid was 1:10 diluted and used as standard quantitative template to make the standard curve for sample detection.</p><p><b>RESULTS</b>The standard curve indicated the linear relationship between Ct (cycle threshold) and template concentration. The clinical samples from 135 cases were detected by this system, 16 cases among 135 were positive.</p><p><b>CONCLUSION</b>The fluorescent quantitative PCR method for the detection of human herpes virus type 6 is simple and accurate, and this method may be helpful to clinical diagnosis.</p>
Assuntos
Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Primers do DNA , Sondas de DNA , DNA Viral , Genética , Herpesvirus Humano 6 , Genética , Reação em Cadeia da Polimerase , Métodos , Púrpura Trombocitopênica Idiopática , Diagnóstico , Virologia , Reprodutibilidade dos Testes , Infecções por Roseolovirus , Diagnóstico , Virologia , Sensibilidade e EspecificidadeRESUMO
<p><b>OBJECTIVE</b>To probe into a treatment method for increasing clinical therapeutic effect on xerophthalmia.</p><p><b>METHODS</b>Forty-five cases were randomly divided into acupuncture group 1, acupuncture group 2 and control group. The acupuncture group 1 were treated with acupuncture based on syndrome types, and the acupuncture group 2 with acupuncture at local acupoints, and the control group with dripping of artificial tears. Local symptoms of eyes, S1T, BUT and FL were observed before and after treatment.</p><p><b>RESULTS</b>S1T and BUT increased after treatment in the two groups (P < 0.01, P < 0.05), but did not significantly change in the control group (P > 0.05). There were significant differences in change of FL as the control group compared with the acupuncture group 1 and 2. The total effective rate was 86.7%, 66.7% and 33.3% in the acupuncture group 1, 2 and the control group, respectively.</p><p><b>CONCLUSION</b>The integral syndrome differentiation acupuncture can improve secretion of tear, increase stability of tear film, improve local symptoms of eyes with a therapeutic effect better than acupuncture at local acupoints and artificial tears.</p>