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1.
Artigo em Inglês | WPRIM | ID: wpr-764368

RESUMO

BACKGROUND AND PURPOSE: It is essential to develop a reliable predictive serum biomarker for Parkinson's disease (PD). The accumulation of alpha-synuclein (αSyn) and up-regulated expression of Rab35 participate in the etiology of PD. The purpose of this investigation was to determine whether the combined assessment of serum αSyn and Rab35 is a useful predictive biomarker for PD. METHODS: Serum levels of αSyn or Rab35 were determined in serum samples from 59 sporadic PD patients, 19 progressive supranuclear palsy (PSP) patients, 20 multiple system atrophy (MSA) patients, and 60 normal controls (NC). Receiver operating characteristics (ROC) curves were calculated to determine the diagnostic accuracy of αSyn or/and Rab35 in discriminating PD patients from NC or atypical parkinsonian patients. RESULTS: The levels of αSyn and Rab35 were increased in PD patients. The serum level of Rab35 was positively correlated with that of αSyn in PD patients. Compared to analyzing αSyn or Rab35 alone, the combined analysis of αSyn and Rab35 produced a larger area under the ROC curve and performed better in discriminating PD patients from NC, MSA patients, or PSP patients. When age was dichotomized at 55, 60, 65, or 70 years, the combined assessment of αSyn and Rab35 for classifying PD was better in the group below the cutoff age than in the group above the cutoff age. CONCLUSIONS: Combined assessment of serum αSyn and Rab35 is a better biomarker for discriminating PD patients from NC or atypical parkinsonian patients, and is a useful predictive biomarker for younger sporadic PD patients.


Assuntos
Humanos , alfa-Sinucleína , Atrofia de Múltiplos Sistemas , Doença de Parkinson , Curva ROC , Paralisia Supranuclear Progressiva
2.
Artigo em Chinês | WPRIM | ID: wpr-698514

RESUMO

BACKGROUND: Phenylephrine has been proved to exert a protective effect on radiant-induced salivary gland and epithelial cell injuries, but its effect on hydrogen peroxide (H2O2)-induced oxidative stress in osteoblasts are not fully understood. OBJECTIVE: To explore the effect of phenylephrine on H2O2-induced oxidative stress in osteoblasts, and to explore the mechanism underlying the regulation by the expression level of nicotinamide phosphoribosyltransferase (Nampt). METHODS: Primary osteoblasts were cultured and randomly divided into four groups: blank control group, H2O2group, phenylephrine group, and combination group (0.5 hour pretreatment of 1×10-5mol/L phenylephrine, and then given 300 μmol/L H2O2). The morphology of osteoblasts was observed at different time points. Osteoblasts were collected after 24-hour culture, and total RNA and protein were then extracted to detect the mRNA and protein expression levels of Nampt by RT-PCR and western blot assay, respectively. RESULTS AND CONCLUSION: Compared with the blank control group, reduced osteoblasts and evident cell shrinks were observed in the H2O2group, while the number of osteoblasts significantly increased in the combined group compared with the H2O2group at 12, 24 and 48 hours of culture. RT-PCR results showed that the mRNA level of Nampt in the H2O2group was reduced by 31.23% of that in the blank control group, while the mRNA level of Nampt in the combination group was dramatically increased by 206.20% of that in the H2O2group at 24 hours of culture (both P < 0.05). Furthermore, western blot assay findings revealed that the protein level of Nampt in the H2O2group was reduced by 67.98% of that in the blank control group, while the protein level of Nampt in the combination group was increased by 152.25% of that in the H2O2group at 24 hours of culture (both P < 0.05). Our results indicate that phenylephrine can alleviate the shrink and atrophy of osteoblasts caused by H2O2, thereby exerting protective effect by up-regulating the mRNA and protein levels of Nampt that may be a regulatory gene.

3.
Saudi Medical Journal. 2014; 35 (5): 435-441
em Inglês | IMEMR | ID: emr-159396

RESUMO

To observe the effect of transfecting the gene human insulin-like growth factor [hIGF]-1 into human umbilical cord blood mesenchymal stem cells [hUCB-MSCs] via non-viral vector. This study was performed in the Affiliated Hospital of Qingdao University, Qingdao, China from June 2012 to May 2013. Twelve hUCB samples were harvested, and isolated in lymphocyte separation medium, and then cultured. Surface antigen expression in MSCs was detected by flow cytometry. Recombinant plasmid pIRES2-enhanced green fluorescent protein [EGFP]-hIGF-1 was transfected into MSCs by X-treme GENE HP DNA transfection reagent. Then, EGFP was observed with reverse fluorescent microscope at different time points. Enzyme-linked immunosorbent assay was used to determine the hIGF-1 protein concentration in supernatants. Immunofluorescence microscopy and reverse transcription polymerase chain reaction were used to detect the expression of hIGF-1 in the hUCB-MSCs. Expression of type II collagen was detected by immunohistochemistry staining. Transfection efficiency was 28.74 +/- 7.31%. The cluster of differentiation [CD]90, CD105, and CD146 expression increased CD34, CD45, and anti-HLA-DR expression decreased. Results of immunofluorescence microscopy and RT-PCR confirmed expression of the hIGF-1 gene. The hIGF-1 protein concentration in the supernatants showed a peak level at 34.63 +/- 1.61 ng/ml 48 hours after transfection. Immunohistochemical analysis of transfected hUCB-MSCs proved that type II collagen could be expressed positively. Human IGF-1 gene can be transfected into hUCB-MSCs, and expressed at a high level with subsequent expression of type II collagen

4.
Artigo em Chinês | WPRIM | ID: wpr-242998

RESUMO

<p><b>OBJECTIVE</b>To study the response of rat bone marrow mesenchymal stem cells (MSCs) to a single period of mechanical strain and expression patterns of transforming growth factor-beta (TGF-beta) and insulin-like growth factor-II (IGF-II) after mechanical stretch.</p><p><b>METHODS</b>Bone marrow MSCs were isolated from SD rats and cultured in vitro. A four-point bending apparatus were used to perform a single period of mechanical strain (2000 microepsilon, 40 min) on MSCs. Cellular proliferation and alkaline phosphatase (ALP) activity of MSCs were examined and gene expression patterns of TGF-beta and IGF-II were detected by SYBR green quantitative real-time RT-PCR.</p><p><b>RESULTS</b>Cell proliferation, ALP activity and expression of TGF-beta and IGF-II were all significantly up-regulated in stretched MSCs when compared with their controls. The mRNA levels of TGF-beta and IGF-II got top increase immediately after mechanical loading and increased about 51.44 and 8.92 folds, respectively, when compared with control cells. Expression of TGF-beta and IGF-II decreased with time and returned to control level at 12 h after mechanical stimulus, despite of a small increase at 6 h.</p><p><b>CONCLUSION</b>The mechanical stretch can promote MSCs proliferation, up-regulate its ALP activity and induce a time-dependent expression increase of TGF-beta and IGF-II which in turn result in osteogenic differentiation of MSCs. Mechanical stimulus is a key stimulator for osteogenic differentiation of MSCs and vital for bone formation in distraction osteogenesis.</p>


Assuntos
Animais , Ratos , Células da Medula Óssea , Diferenciação Celular , Proliferação de Células , Fator de Crescimento Insulin-Like II , Células-Tronco Mesenquimais , Osteogênese , Osteogênese por Distração , RNA Mensageiro , Ratos Sprague-Dawley , Somatomedinas , Fator de Crescimento Transformador beta
5.
Artigo em Chinês | WPRIM | ID: wpr-248269

RESUMO

<p><b>OBJECTIVE</b>To evaluate the osteoblastic differentiation and compare the difference in the gene expression of rat bone marrow mesenchymal stem cells (MSCs) affected by a single period of mechanical strain.</p><p><b>METHODS</b>Bone marrow MSCs were harvested from the femurs and tibiae of SD rats and cultured in vitro. A four-point bending apparatus were used to perform a single 40-minute period of 2,000 microepsilon mechanical strain on these MSCs. The proliferation of the MSCs was tested by MTT on scheduled date, and the osteoblastic differentiation of the MSCs was measured by testing the expression of osteocalcin and alkaline phosphate (ALP) activity of these cells. In addition, we have investigated the possible mechanisms underlying the action of the single 40-minute period of 2,000 microepsilon mechanical strain on these MSCs, after profile blotted and handled by bioinformation, the gene expressions of these two periods of MSCs were examined.</p><p><b>RESULTS</b>The MSCs have grown well in vitro. Our experiment showed that mechanical environment did not weaken the proliferation of the MSCs. However, the ALP activity and the expression of osteocalcin were significantly up-regulated by the 2,000 microepsilon mechanical strain. Using the 27 K Rat Genome Array, 416 different expressions were found. The rate of different genes was 2.8%, of which the expressions of 247 genes increased (61 genes remarkably increased) and 169 genes decreased (74 genes remarkably decreased) in these two periods of MSCs.</p><p><b>CONCLUSION</b>Mechanical strain induced the osteoblastic differentiation of the MSCs, which may be attributed to the different gene levels.</p>


Assuntos
Animais , Ratos , Fosfatase Alcalina , Células da Medula Óssea , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Células-Tronco Mesenquimais , Osteoblastos , Osteocalcina , Ratos Sprague-Dawley , Transcriptoma
6.
Artigo em Inglês | WPRIM | ID: wpr-269709

RESUMO

<p><b>AIM</b>Understanding the response of mesenchymal stem cells (MSCs) to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis.</p><p><b>METHODOLOGY</b>In this study, a single period of cyclic mechanical stretch (0.5 Hz, 2,000 microepsilon) was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase (ALP) activity was examined. The mRNA expression of six bone-related genes (Ets-1, bFGF, IGF-II, TGF-beta, Cbfa1 and ALP) was detected using real-time quantitative RT-PCR.</p><p><b>RESULTS</b>The results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up-regulate the expression of these genes. A significant increase in Ets-1 expression was detected immediately after mechanical stimulation, but Cbfa1 expression became elevated later. The temporal expression pattern of ALP coincided perfectly with Cbfa1.</p><p><b>CONCLUSION</b>The results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone-related genes may play different roles in the response of MSCs to mechanical stimulation.</p>


Assuntos
Animais , Ratos , Fosfatase Alcalina , Antígenos de Superfície , Fenômenos Biomecânicos , Células da Medula Óssea , Fisiologia , Técnicas de Cultura de Células , Diferenciação Celular , Fisiologia , Proliferação de Células , Subunidade alfa 1 de Fator de Ligação ao Core , Fator 2 de Crescimento de Fibroblastos , Fator de Crescimento Insulin-Like II , Células-Tronco Mesenquimais , Fisiologia , Osteoblastos , Fisiologia , Osteogênese por Distração , Células-Tronco Pluripotentes , Fisiologia , Proteína Proto-Oncogênica c-ets-1 , Estresse Mecânico , Fator de Crescimento Transformador beta , Regulação para Cima , Fisiologia
7.
Artigo em Chinês | WPRIM | ID: wpr-329974

RESUMO

<p><b>OBJECTIVE</b>To explore the response of rat bone marrow mesenchymal stem cells (MSCs and calvarial osteoblasts to mechanical strain and the consequent changes of cytoskeleton F-actin.</p><p><b>METHODS</b>Bone marrow MSCs and calvarial osteoblasts were isolated from SD rats and cultured in vitro. Mechanical stretch was performed on passage 3 cells at 2 000 microepsilon for 0, 2, 6 and 12 hours using four-point bending system. The response of cells and the distribution of F-actin were observed using fluorescent staining under laser scanning confocal microscope and the morphological parameters were quantified using image analysis software Laserpix.</p><p><b>RESULTS</b>Under mechanical stretch, the fluorescent staining decreased obviously at both MSCs and osteoblasts, and F-actin filaments were rearranged and became tenuous, thinner, and abnormally distributed. The outline of nucleus became unclear and apoptotic changes were observed at some of both cells. Cellular size decreased more significantly in MSCs than in osteoblasts. Quantity analysis showed that total area of cells, total fluorescent density and green fluorescent density (F-actin) were all significantly decreased in MSCs (P < 0.05 or P < 0.01), and total fluorescent density, green fluorescent density and red fluorescent density (nuclei) did also in osteoblasts (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>Mechanical stretch caused extensive response on both MSCs and osteoblasts which led to the rearrangement of F-actin filament and apoptosis in some of these cells. MSCs were more sensitive to mechanical strain than osteoblasts.</p>


Assuntos
Animais , Ratos , Citoesqueleto de Actina , Metabolismo , Actinas , Metabolismo , Células da Medula Óssea , Células Cultivadas , Citoesqueleto , Células-Tronco Mesenquimais , Microtúbulos , Osteoblastos , Estresse Mecânico
8.
Zhonghua Yu Fang Yi Xue Za Zhi ; (12): 159-161, 2004.
Artigo em Chinês | WPRIM | ID: wpr-291799

RESUMO

<p><b>OBJECTIVE</b>This study is to investigate the effect of tea polyphenols and tea pigments on telomerase activity of human liver cancer cell line, HepG2 cells.</p><p><b>METHODS</b>TRAP-PCR-ELISA was applied to investigate the telomerase activity.</p><p><b>RESULTS</b>Telomerase was positive in tea polyphenols treated groups, tea pigments treated groups and blank control group. Telomerase activities (A(450 approximately 690) values) were 1.56 and 1.46 in 50 mg/L and 100 mg/L tea polyphenols-treated groups, 1.55 and 1.49 in 50 mg/L and 100 mg/L tea pigments-treated groups, respectively. The results showed that telomerase activity was significantly inhibited by tea polyphenols and tea pigments treatment as compared with the blank control group (A(450 approximately 690) = 2.11).</p><p><b>CONCLUSIONS</b>Tea polyphenols and tea pigments could significantly inhibit telomerase activity of HepG2 cells, and telomerase activity may be a useful biomarker for cancer chemoprevention.</p>


Assuntos
Humanos , Biomarcadores Tumorais , Metabolismo , Carcinoma Hepatocelular , Patologia , Flavonoides , Farmacologia , Neoplasias Hepáticas , Patologia , Fenóis , Farmacologia , Pigmentos Biológicos , Farmacologia , Polifenóis , Chá , Química , Telomerase , Metabolismo , Células Tumorais Cultivadas
9.
Artigo em Chinês | WPRIM | ID: wpr-676160

RESUMO

Objective To investigate the change of the basic fibroblast growth factor(bFGF) leve in human detrusor muscle(DM)in bladder outlet obstruction(BOO)due to benign prostatic hyperplasia(BPH)and its implication.Methods Fifty-four patients with BPH were divided into two groups:the obstructive DM stability and instability groups;and 15 men with bladder tumor who underwent operation in the same period were enrolled in the control group.The bFGF mRNA level in DM was measured by reverse transcription and polymerase chain reaction(RT-PCR)and the bFGF protein level was measured by immunohistochemical staining method.Results The bFGF-mRNA expression level of bladder smooth muscle cells was significantly lower in the control group than that in the obstructive DM stability and instability groups(all P<0.05),but there was no significant difference between the obstructive DM stability and instability groups(P>0.05). Conclusions The expression level of bFGF mRNA in bladder DM is elevated in BOO due to BPH,but there is little or no correlation between the increased expression of bFGF mRNA and detrusor muscle instability.

10.
Zhonghua Yu Fang Yi Xue Za Zhi ; (12): 225-228, 2002.
Artigo em Chinês | WPRIM | ID: wpr-257261

RESUMO

<p><b>OBJECTIVES</b>This study is to investigate the effects of tea polyphenols and tea pigments on cell cycle regulators in rat liver precancerous lesions.</p><p><b>METHODS</b>The modified Solt-Farber precancerous liver rat model was used. Rats were given water, tea polypheol solution (0.1%) or tea pigment solution (0.1%) throughout the whole experiment (56 days). Cyclin D1, P21(WAF1/CIP1), GADD45 and PCNA protein expression were detected by Western blotting and the RT-PCR method was applied to study the expression of Cdk4.</p><p><b>RESULTS</b>Cyclin D1, Cdk4 and PCNA expressions were significantly inhibited, and the expression of P21(WAF1/CIP1) and GADD45 were significantly induced by tea polyphenols and tea pigments treatments.</p><p><b>CONCLUSION</b>Tea polyphenols and tea pigments induced cell cycle arrest and inhibited cell proliferation by regulating cell cycle regulators.</p>


Assuntos
Animais , Masculino , Ratos , Western Blotting , Proteínas de Ciclo Celular , Genética , Metabolismo , Ciclina D1 , Metabolismo , Quinase 4 Dependente de Ciclina , Inibidor de Quinase Dependente de Ciclina p21 , Quinases Ciclina-Dependentes , Genética , Ciclinas , Metabolismo , Flavonoides , Peptídeos e Proteínas de Sinalização Intracelular , Neoplasias Hepáticas , Genética , Metabolismo , Patologia , Fenóis , Farmacologia , Pigmentos Biológicos , Farmacologia , Polímeros , Farmacologia , Polifenóis , Lesões Pré-Cancerosas , Genética , Metabolismo , Patologia , Antígeno Nuclear de Célula em Proliferação , Metabolismo , Proteínas , Proteínas Proto-Oncogênicas , RNA Mensageiro , Genética , Metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Chá , Química
13.
Artigo em Coreano | WPRIM | ID: wpr-186423

RESUMO

No abstract available.


Assuntos
Dermatomiosite
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