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BACKGROUND:Leukemia comes from the damage to bone marrow microenvironment, and changes in bone marrow microenvironment lead to some effects on biological characteristics and behavior of leukemia cel s. OBJECTIVE:To explore the effect of bone marrow mesenchymal stem cel s from children with leukemia on K562/AO2 cel line growth, proliferation and apoptosis. METHODS:K562/AO2 cel s were cultured alone in vitro, or co-cultured with bone marrow mesenchymal stem cel s from leukemia children. The living cel s in the two groups were counted using trypan blue staining, and the cel growth curves were described. PI single staining method was used to detect the cel cycle, and cel apoptosis was detected by V/PI Annexin method in the two groups. RESULTS AND CONCLUSION:After single suspension culture, the cel s showed no marked changes within the first 3 days, but began to proliferate fast at 4 days and reached the proliferation peak at 6 days. Co-cultured cells showed a gentle growth curve with no presence of obvious proliferation peak. Compared with the co-culture group, the proportion of G0/G1 phase cel s was significantly lower, but the proportion of S phase cells was significantly higher in the single culture group (P0.05). All these findings indicate that co-culture with bone marrow mesenchymal stem cel s from leukemia children inhibits the proliferation of K562/AO2 cells through G0/G1 phase arrest, and also resists apoptosis in K562/AO2 cells.
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OBJECTIVE:To observe clinical efficacy and safety of pidotimod combined with montelukast sodium in the treat-ment of children with bronchial asthma. METHODS:120 children with bronchial asthma were randomly divided into control group and observation group,with 60 cases in each group. Both group were given routine treatment as aerosol inhalation of glucocorticoid and anti-infective treatment. Control group was additionally given Montelukast sodium chewable tablet 5 mg orally,qd,at bed-time;observation group was additionally given Pidotimod capsule 0.4 g,qd,on the basis of control group. Both group received treatment for consecutive 3 months. Clinical efficacies of 2 groups were observed as well as IL-4,IFN-γ,IgE,T lymphocyte sub-set and lung function indexes of 2 groups before and after treatment. The occurrence of ADR were compared between 2 groups. RE-SULTS:The effective rate of observation group was 88.3%,which was significantly higher than that of control group (61.7%). There was no statistical significance in IL-4,IFN-γ,IgE,T lymphocyte subset and lung function indexes between 2 groups before treatment(P>0.05). After treatment,IL-4,IFN-γ,IgE,CD4+,CD4+/CD8+ and lung function of 2 groups were improved signifi-cantly,and the observation group was significantly better than the control group,with statistical significance(P0.05). CONCLUSIONS:Pidotimod combined with monte-lukast sodium is effective for children with bronchial asthma,and can significantly relieve airway inflammation,enhance immunity and improve lung function with good safety.