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1.
National Journal of Andrology ; (12): 436-442, 2003.
Artigo em Chinês | WPRIM | ID: wpr-238003

RESUMO

<p><b>OBJECTIVE</b>To develop a real-time fluorescent PCR protocol suitable for the routine screening of AZFc/DAZ microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.</p><p><b>METHODS</b>A set of real-time fluorescent PCR was established. Eighty-seven azoospermic and ligozoospermic patients undergoing ICSI in the IVF center and 30 azoospermic men undergoing testicular biopsy in the clinic of urology surgery were screened for AZFc/DAZ microdeletions of Y chromosome.</p><p><b>RESULTS</b>Eleven cases (9.4%) of AZFc/DAZ microdeletions were found in 117 cases of azoospermic and oligozoospermic patients by screening of realtime fluorescent PCR. Four cases (6.6%) were found in 61 oligozoospermic patients, and 7 cases (12.5%) were found in 56 azoospermic patients.</p><p><b>CONCLUSION</b>The real-time fluorescent PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome, which yields identical results to those of the multiplex PCR.</p>


Assuntos
Humanos , Masculino , Deleção Cromossômica , Cromossomos Humanos Y , Proteína 1 Suprimida em Azoospermia , Fluorescência , Infertilidade Masculina , Genética , Reação em Cadeia da Polimerase , Métodos , Proteínas de Ligação a RNA , Genética
2.
Chinese Journal of Medical Genetics ; (6): 357-359, 2003.
Artigo em Chinês | WPRIM | ID: wpr-329461

RESUMO

<p><b>OBJECTIVE</b>To develop a multiplex PCR protocol, which could be suitable for routine screening of microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.</p><p><b>METHODS</b>Five multiplex sets were established. Eighty-seven azoospermic and oligozoospermic patients undergoing intracytoplasmic sperm injection (ICSI) in the in vitro fertilization (IVF) center and 30 azoospermic men undergoing testicular biopsy in the clinic of Urology Surgery were screened for microdeletions of Y chromosome.</p><p><b>RESULTS</b>A total of 19 (16.2%) cases of microdeletions were found in 117 azoospermic and oligozoospermic patients by screening of Y chromosome microdeletions. Of these, 11 cases (18.0%) were found in 61 oligozoospermic patients, and 8 cases (14.3%) were found in 56 azoospermic patients.</p><p><b>CONCLUSION</b>The multiplex PCR protocol presented in this study is an easy-to-do and reliable method for detecting microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic and oligozoospermic patients is essential.</p>


Assuntos
Feminino , Humanos , Masculino , Deleção Cromossômica , Cromossomos Humanos Y , Genética , Testes Genéticos , Métodos , Infertilidade Masculina , Diagnóstico , Genética , Reação em Cadeia da Polimerase
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