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Acta Academiae Medicinae Sinicae ; (6): 283-286, 2007.
Artigo em Chinês | WPRIM | ID: wpr-229986

RESUMO

<p><b>OBJECTIVE</b>To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).</p><p><b>METHOD</b>Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.</p><p><b>RESULT</b>The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.</p><p><b>CONCLUSION</b>Single cell fluorescent PCR is a stable and reliable approach for the PGD.</p>


Assuntos
Feminino , Humanos , Gravidez , Corantes Fluorescentes , Técnicas de Diagnóstico Molecular , Métodos , Linhagem , Doenças Renais Policísticas , Diagnóstico , Genética , Reação em Cadeia da Polimerase , Métodos , Diagnóstico Pré-Implantação , Métodos
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