The hypoglycemic effect of gynostema pentaphylum on mouse and rat

Primary study showed hypoglycemic effect of gynostema pentaphyllum (GP) on mice. Objective: (I) To determine the hypoglycemic effect of (GP) and (2) To look for effective mechanism of GP on normal and diabetic mice and rats. Method: The ethanol extract of GP were introdiced to normal and pre diabetic & diabectic mice or rats. The blood glucose levels were measured at just (0h) before and at 2h, 4h and 6h after oral use or 1h, 2h and 3h after intraperitoneal use of GP. Results: On normal mice, 300 mg/kg-ip of GP decreased the blood glucose level with max. 33% at 3rd hour; 1500 mg/kg-po. with 20% at 6th hour; the dose of 500 mg/kg x 7 days consecutive decreased this level with 24%. On normal Wistar rats 500 mg/kg-po, diminished glycemia with max. 20% at 4th hour. But on genetic diabetic rats (GK rats), the doses 500 mg/kg-po, and 1000 mg/kg-po, decreased the blood glucose concentrations by 22% and 36%, respectively at the same time. In the glucose toleance test on mice the dose 1000 mg/kg-po, inhibited the hyperglycemic effect with 55% (after 30 minutes) and 63% (after 60 minutes) in comparison to control group. Conclusion: GP has the mild hypoglycemic effect on normal mouse/rat, but it causes more higher effect on mouse/rat having hyperglycemia. Thus, beside the insulin secreting stimulation, GP may sensitive the target tissues to insulin.

Study on semi-chronic toxicity of cisplatin DLTW 1 on liver and kineys

Cisplatin was intravenously injected at the dose of 0.2 mg/kg (equivalent to 1/10 of the maximum non-toxicating dose in mice) four 5-day episodes with the interval of 5 days caused a slight increase in the serum creatinine and urea levels. Similar effects were also observed when cisplatin was IV administered at 0.3 mg/kg/24h for two 5-day episodes with the interval of 10 days. Other mild side effects towards liver and some organs were also observed in about 50% of the mice population. Most of the side effects significantly subdued in both groups 15 days after stopping drug, except damages in hepatic histology, which recovered more slowly

The hypoglycemic effect of atractylodes macrocephala, lycium sinense and seoparia dulcis on mice

Objectives: To evaluate the hypoglycemic effect of these herbal medicines' on normal mice. Methods: the ethanol extracts of these plants have been introduced intraperitoneally to mice with the dose of 500 mg/kg of body weight. The blood glucose levels were determined by one - touch glucose meter at just (0h) before and at 1h, 2h, 3h, 4h and conclusion after drug administration. Results: Lycium sinense Mill lowered the glycemia by more than 30% in comparison to that of initial level. Separia dulcis, however, caused hyperglycemia (+ 62%) at the first hour then at the 3rd and 4th hour the blood glucose was diminished with>30%. Atractylodes macrocephala did not lower the glycemia.

The anti inflammatory and diuretic effects and acute toxicity of aqueous extract of psychotria morindoides hutch on rat and mice

The 1:1 aqueous extract of Radix Psychotria morindoides Hutch – Rubiacease with an oral dose of 10g/kg body weight of rat: Has no acute and anti-inflammatory effect on carrageenin – indecued edema in hind paw, but it reduces the asbestos induced granuloma as a subchronic inflammation medel. Increases the excretion of urine up to 83% at the sixth hour after drug ingestion. DL50 of the aqueous extract could not be determined because of its very low toxicity.

To evaluate the hypoglycemic effect of smilax glabra-roxb per oral route in the mice and its influence on the glucose tolerance test

Cryophylisat powder of ethanolic fluid extract of Smilax glabra (SG) was administered orally on mouse with a single dose of 1g/kg of body mass. Hypoglycemic effect exerted with 10.58% approximatively. Continous use of 7 days of this dose increased the hypoglycemic action. Oral SG effects manifested lately with less level than injectable SG. Apart from the increase of synthetization capacity of glycogene, SG promote the sensitivity of the tissues to insuline.

Subchronic toxicity of smilax glabra roxb (SG) on rabbit

Cryophylisat powder of ethanolic fluid extract of Smilax glabra with the oral dose of 1.25g and 2.5g/kg of body mass (5-10 times of active dose on hypoglycemic effect was administered in rabbit in 15-30 days continously. These doses did not influenced on the body mass, the hematologies indices as well as the functional indices of lever and kidney. However, in microscopic image, there are some degenerative mainfestations of low levels of liver cells among two groups using the studied preparation.

Screening study on the hypoglycemic effect of four herbal medicines in Vietnam

On rat, among 4 these herbal medicine plants, only Rehmania flutinosa does not decrease significantly the blood sugar level with the oral doses of 1000-1500 mg/kg body weight and with intraperitoneal doses of 200-300mg/kg. Oral use of Anemarrhena asphodeloides and Angiopteris evecta decreases blood sugar at the doses of 100-1.500mg/kg. Intraperitoneal use of the doses of 200-300mg/kg Anemarrhena asphodeloides, Angiopteris evecta and gynoitema pentaphyllum lower glycemia by above 25% in comparison with the pretreated level.

Effects of Morinda sp. on Liver Injury Induced by Paracetamol in Rats

Effects of aqueous extracts from the root of Morinda sp. were studied on liver injury induced by a high dose of paracetamol (acetaminophen) in rats. The results showed that the extract had a hepatoprotective effect at a dose of 10g/kg of body weight. The effect was similar to that of silymarin at a dose of 25mg/kg of body weight in terms of AST, ALT activities, total serum cholesterol and protein concentrations as well as macro-and microscopic histological studies of the liver.