Analysis Of Y-Linked Lineage Markers In Indian Populations.

Recently, Y-linked markers have been found extremely useful tools in population genetics and evolutionary biology. We have studied a tetranucleotide marker, DYS19 and a bialleleic marker DYS287, an Alu polymorphic marker (YAP) in five ethnic groups of India. Blood samples were collected from 215 random healthy males and DNA was extracted by using a simple salt precipitation method. DNA samples, after amplification by polymerase chain reaction (PCR), were electrophoresed. The detection of alleles was done by silver staining and reconfirmed by using a fragment analyser programme in an ALF express sequencer (Pharamacia). A total of four alleles were observed at DYS19 locus in all the five ethnic groups. Allele 194bp was found to be the predominant one in all the groups. The absence of YAP was found in all the groups.

D1S80 Minisatellite - Polymorphism In Five Ethnic Populations Of Maharashtra And Kerala.

The minisatellite locus D1S80 was studied in five ethnic populations, namely, Konkanastha Brahmins and Marathas of Maharashtra; Nairs, Ezhavas and Muslims from Kerala, to compare the allele and genotype frequencies. DNA isoleted from blood samples collected from 364 unrelated healthy donors was amplified by PCR and subjected to polyaerylamide gel electrophones. A total of 20 alleles and 71 genotypes were observed from the above population groups. A bimodal pattern was observed in the allele frequency distribution representing 18 and 24 as predominant ones. The observed heterozygosity was in the range of 65% to 82%. All the five population groups conformed to Hardy- Weinberg equilibrium expectations.

Studies On ApoB VNTR In Two Maharashtrian Ethnic population Groups.

The ApoB VNTR locus was analyzed in two distinct ethnic groups of Maharashtra to determine, the distribution of allele and genotype frequencies. Blood samples were collected from 183 random, unrelated, healthy donors. DNA was extracted by using a simple salt precipitation method, amplified by polymerase chain reaction (PCR) and the products were electrophoresed in 4% PAGE followed by silver staining. A total of 14 alleles and 38 genotypes were observed. Allele 37 and 39 were found to be the predominant alleles showing a bimodal distribution. Both the population groups conformed to Hardy-Weinberg Equilibrium expectations.