Study on the Promoter Region-149C＞T and-579G＞T Polymorphism of DNMT3B with Genetic Susceptibility to Esophageal Cancer of Han Population in Jiangsu Suqian / 现代检验医学杂志

Objective To investigate the association of DNA methyltransfcrase 3B (DNMT3B) promoter region single nucleo tide polymorphism (SNP)-149C＞T and-579G＞T with genetic susceptibility of esophageal cancer (EC) of Han population in Jiangsu Suqian.Methods Gcnotypes of the-149C＞T and 579G＞T locus in DNMT3B promoter region were examinedby polymerase chain reaction with restriction fragment length polymorphism (PCR RFLP) in 246 esophageal cancer patients and 240 healthy controls.Results In-149C＞T site,the distributions of genotypes TT compared with CT in the EC group and the controls were no significant difference (x2 =0.089,P＞0.05).In-579G＞T site,the distributions of genotypes TT compared with GT+GG in the EC group and the controls were no significant difference (x2 =0.649,P＞0.05).When strat ified by age and gender,there was no significant difference in the EC group and the controls in both two sites (x2 =0.044～0.876,all P＞0.05).Conclusion The DNMT3B promoter region-149C＞T and-579G＞T were not associated with the genetic susceptibility of esophageal cancer.

Prevalence of Helicobacter pylori cagA, vacA, and iceA genotypes in children with gastroduodenal diseases / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the prevalence of cagA, vacA, and iceA genotypes in the isolated strains of Helicobacter pylori (H.pylori) from children with gastroduodenal diseases in Jiangxi, China, as well as the association between cagA, vacA, and iceA genotypes and the type of gastroduodenal diseases.</p><p><b>METHODS</b>The samples of gastric antral mucosa were collected from 316 children with gastroduodenal diseases in Jiangxi, and a total of 107 strains of H.pylori were isolated. The genomic DNA of these strains was extracted, and PCR was used to determine the ureA, cagA, vacA, and iceA genotypes.</p><p><b>RESULTS</b>Of all the 107 isolated strains of H.pylori, the detection rates of ureA and cagA genes were 100% (107/107) and 94.4% (101/107) respectively. The overall detection rate of vacA gene was 100% (107/107), and the detection rates of vacAs1a, vacAs1c, vacAm1, and vacAm2 genes were 74.8% (80/107), 25.2% (27/107), 29.9% (32/107), and 69.2% (74/107) respectively, with both vacAm1 and vacAm2 genes detected in 0.9% (1/107) of all H.pylori strains. In the chimera of vacA gene, the detection rates of vacAs1a/m1, vacAs1a/m2, vacAs1c/m1, and vacAs1c/m2 genes were 26.2% (28/107), 51.4% (55/107), 3.7% (4/107), and 17.8% (19/107) respectively (P<0.001). The detection rates of iceA1 and iceA2 genes were 79.4% (85/107) and 9.3% (10/107), respectively (P<0.001), and both iceA1 and iceA2 genes were detected in 7.5% (8/107) of all strains. The detection rates of the genotypes of H.pylori showed no significant differences between the peptic ulcer, chronic gastritis, and duodenal bulbar inflammation groups (P>0.05).</p><p><b>CONCLUSIONS</b>The dominant genotypes of H.pylori are cagA, vacAs1a/m2, and iceA1, and there are mixed infections with H.pylori strains of different genotypes in children with gastroduodenal disease from Jiangxi, China. The genotypes of H.pylori are not associated with the type of gastroduodenal disease.</p>

A study on relationship between type II diabetes, hyper lipid level and carotid artery atherosclerosis by ultrasonography / 预防医学

Objective ToevaluatetherelationshipbetweentypeIIdiabetes,hyperlipidlevelandcarotidartery arthrosclerosisbyultrasonography.Methods Atotalof150retiredofficerswereselected,ofwhich63diabeticpatients were divided into case group while 87 subjects free of diabetes were divided into control group.Blood laboratory examination wasconductedandthemanifestationofcarotidarterywasexaminedbyultrasonography.Results TCH,TG,LDLand ApoB of case group were significantly higher than those in control group (P<0.05 ).The intimamedia thickness (IMT)of carotid artery in case group was increased significantly (P<0.05 ).Moreover,arteries narrowing and plaque were more than those in control group (both P <0.05 ).T peak velocity in carotid artery and internal carotid were of statistical significance between two groups (P<0.01 ).Furthermore,multiple linear regression analysis considering the degree of carotid artery arthrosclerosis and serum lipid showed that multiple correlation coefficient of case group was 0.870 (P<0.01)and0.902(P<0.01)ofcontrolgroup.Conclusion Hyperlipidemiamayhavethepromoteeffectontheformation of arteriosclerosis plaque and ultrasonography could be one of the effective methods to detect early vessel lesion of diabetic patients.

Effect of digital radiography processing parameters on digital chest radiograph for occupational exposed workers / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the effect of different processing parameters of digital radiography (DR) on the image quality of digital chest radiograph in dust-exposed workers.</p><p><b>METHODS</b>One hundred and five dust-exposed workers underwent both high-KV radiography and DR to obtain chest radiographs; the image processing parameters were set by the conventional processing method for digital chest radiograph (method A) and the processing method based on the special requirements of occupational diseases (method B). With the high-KV chest radiograph as the reference, the image qualities at 10 anatomic sites of DR image were graded. The images acquired by DR and high-KV radiography were compared, and the DR images acquired by methods A and B were also compared.</p><p><b>RESULTS</b>For method A, the scores at the 10 anatomic sites of DR image were mostly 0 and +1, accounting for over 88%, and the mean score was 0.23 ∼ 0.65, there was a significant difference between the mean score of DR image and the score of high-KV image (P < 0.001). For method B, the scores at the 10 anatomic sites of DR image were mostly 0, accounting for over 65%, and the mean score was -0.01∼ +0.02 except at the pleura and chest wall; there was no significant difference between the mean score of DR image and the score of high-KV image (P > 0.05). There were significant differences in the scores at the 10 anatomic sites between the DR images acquired by methods A and B (P < 0.01).</p><p><b>CONCLUSION</b>The DR images acquired based on different processing parameters are different. The quality of DR image acquired by the processing method based on the special requirements of occupational diseases is similar to that of high-KV image at the anatomic sites.</p>

Effect of tube voltage on digital chest radiograph for phantom and occupational exposed workers / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the image quality of different tube voltage on digital chest radiograph for contrast detail phantom (CDRAD2.0) and occupational exposed workers.</p><p><b>METHODS</b>The CDRAD2.0 phantom DR images of high KV and different tube voltages were analyzed by 3 readers, image quality figure (IQF) were calculated and compared; at the same time 136 exposed workers were examined with high-kV and DR chest radiograph of different tube voltages. Contrast to high-KV images, 10 anatomic sites were scored .The image differences were compared between DR and high-kV.</p><p><b>RESULTS</b>On CDRAD2.0 phantom, the IQF value of DR images in 3 readers were minimum in the condition of 120 kV, average value was 22.25. The analysis of variance in model with random effects, the mean IQF value of different tube voltage DR image had a significant difference (F = 13.775, P<0.01); By Dunnett t-tests analysis, the mean IQF value of DR image in 120 kV and high kilovoltage had no difference (t = -0.58, P = 0.979); On clinical cases, the DR image of 120 kV showed the closest anatomy to the high KV, the mean had no significant difference with 0 (P > 0.05) with single sample t test.</p><p><b>CONCLUSION</b>On the CDRAD2.0 phantom or clinical exposed workers, the DR image quality of 120 kV tube voltage equals to high-KV basically.</p>

Acupuncture for chronic pelvic pain syndromes (CPPS) and its effect on cytokines in prostatic fluid / 中国针灸

<p><b>OBJECTIVE</b>To explore the effect of acupuncture on chronic pelvic pain syndromes (CPPS), and its therapeutic mechanism.</p><p><b>METHODS</b>Fourty-seven cases of CPPS were treated with electroacupuncture on Zhongji (CV 3), Guilai (ST 29), Yinlingquan (SP 9), Sanyinjiao (SP 6), Guanyuan (CV 4), Shuidao(ST 28), Xuehai (SP 10) and Taichong (LR 3) as main acupoints. Chronic Prostatitis Symptom Index (CPSI) was adopted to grade the severity of pain or discomforts. Additionally, the levels of Interleukin-8 (IL-8), Interleukin-10 (IL-10) and Tumor necrosis factor-alpha (TNF-alpha) in prostate fluid were detected and the correlation between those changes and pain score was analyzed.</p><p><b>RESULTS</b>After treatment, pain or discomfort score in CPSI decreased remarkably as compared with that before treatment (P < 0.01). The levels of IL-8, IL-10 and TNF-alpha were lower than those before treatment (P < 0.01, P < 0.05). The positive correlation was obtained between IL-10 level and pain score (P < 0.05). The total effective rate was 89.4% (42/47).</p><p><b>CONCLUSION</b>Acupuncture has significant efficacy on CPPS through reducing IL-10 level to ease pain, and reducing the levels of IL-8 and TNF-alpha to relieve inflammatory reaction.</p>

Acupoint-injection of BCG polysaccharide nuclear acid for treatment of condyloma acuminatum and its immunoregulatory action on the patient / 中国针灸

<p><b>OBJECTIVE</b>To probe into immunological mechanisms and clinical therapeutic effect of acupoint-injection of BCG polysaccharide nuclear acid (BCG-PSN) for treatment of condyloma acuminatum (CA).</p><p><b>METHODS</b>Two hundred cases were randomly divided into 4 groups. After removed the CA by laser, the treatment group (group A) was treated with acupoint-injection of BCG-PSN, the control group I (group B) with intramuscular injection of BCG-PSN, the control group II (group C) with intramuscular injection of interferon, and the blank control group (group D) with no treatment. The levels of cellular immune function were detected before treatment and after treatment of 6 months, and the cases of relapse were recorded.</p><p><b>RESULTS</b>The cured rate of 94.3% in the group A was significantly higher than 78.0% in the group B, 80.4% in the group C and 78.2% in the group D, with significant differences (P < 0.05); in the group A, CD4+ percent increased, CD8+ percent decreased, CD4+ /CD+ ratio increased, and NK cell activity increased with a low relapse rate, and with significant differences as compared with the control groups (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>Acupoint-injection of BCG-PSN has a better therapeutic effect and it can obviously reduce the recurrence rate of CA. The cellular immunoregulatory action is one of the mechanisms of this therapy in preventing relapse of CA.</p>

Cardiovascular circulation feedback control treatment instrument / 中国医疗器械杂志

The cardiovascular circulation feedback control treatment instrument (CFCTI) is an automatic feedback control treatment system, which has the function of monitoring, alarming, trouble self-diagnosis and testing on the line in the closed loop. The instrument is designed based on the successful clinical experiences and the data are inputted into the computer in real-time through a pressure sensor and A/D card. User interface window is set up for the doctor's choosing different medicine. The orders are outputted to control the dose of medicine through the transfusion system. The response to medicine is updated continually. CFCTI can avoid the man-made errors and the long interval of sampling. Its reliability and accuracy in rescuing the critical patients are much higher than the traditional methods.

Effect of megace on the toxicity reaction in chemotherapy of tumor / 中国康复理论与实践

@#ObjectiveTo observe the effect of megace (MA) on the survival condition of cancer patients in chemotherapy (CT) periods.Methods92 patients with cancer were divided into the MA group (treated with MA+CT) and control group (treated only with CT). The changing of the appetite, normal food amount, weight, gastrointestinal reaction and whole body conditions of two groups were evaluated.ResultsIn the MA group, 52.2% patients had appetite improvement, 47.8% had more food amount, 45.7% gained more weight, 50% had no obvious gastrointestinal reaction such as vomiting and nausea, and 50% had improve the survival condition according to the Karnofsky performance status (KPS) scores (increment >10). In the control group, only 6.5% had appetite improvement, 4.3% had more food amount, 13% gained more weight, 28.3% had no vomiting and nausea, and 15.2% had improved the survival condition. There was a significant difference between two groups (P<0.01).ConclusionMegace is able to reduce nausea and vomiting caused by CT, improve appetite, increase patients weight, protect bone marrow from the inhibition of CT, improve the life quality of cancer patients, and has no evident side-effects.