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Objective:To explore the application of transverse tibial bone transport microangiogenesis combined with vacuum drainage in the treatment of diabetic foot ulcer.Methods:During April 2018 to April 2019, the clinical data of 87 patients with diabetic foot ulcer who were performed transverse tibial bone transport microangiogenesis combined with vacuum drainage in Changxing County Hospital of Traditional Chinese Medicine of Zhejiang Province were collected. In this study, there were 64 cases of males and 23 cases of females, aged 43-84 years, and average age was (64.5 ± 8.4) years. Right foots of 42 cases and left foots of 45 cases received operation. The diabetic course was (13.7 ± 6.2) years. The area of ulcer was 2.2 cm × 3.0 cm-7.5 cm × 5.7 cm. According Wagner grade, 16 case was Ⅱ garde, 38 cases was Ⅲ grade, 30 cases was Ⅳ grade, and 3 cases was Ⅴ grade. All patients were given treatment of anti-infection, monitoring of blood glucose and correcting hypoproteinemia. Ankle-brachial index(ABI) and Michigan Neuropathy Screening Instrument (MNSI) score were used to evaluate the recovery of peripheral vessel and nerve before operation and 3 months after operation. All patients were evaluated by angiography or color Doppler ultrasound after operation.Results:All patients were followed up for 3-24 months with average 13.6 months, and the wound surface were healed. At 3 months after operation, the ABI increased from 0.59 ± 0.19 to 0.89 ± 0.28, the scores of MNSI decreased from (5.81 ± 1.60) scores to (4.42 ± 1.25) scores, and there were significant differences ( P<0.05). The postoperative angiography and vascular ultrasound of 74 cases showed satisfied regeneration of micro-vessel. Conclusions:Transverse tibial bone transport microangiogenesis combined with vacuum drainage can rebuild the micro-vessel in the treatment of diabetic foot ulcer, and improve the wound surface healing. It is an effective method in treatment of diabetic foot ulcer.
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Objective To explore the clinical effect of operative treatment for grade Ⅳ pronation-external rotation ankle injury.Methods 60 patients with pronation extorsion type Ⅳ degree of ankle injury were selected as the research subjects.According to the digital table,they were divided into two groups,30 cases in each group.The group A adopted ordinary steel plate,the group B adopted locking plate fixation.The clinical curative effect,postoperative recovery index,AOFAS score and complications were observed.Results The total effective rate of group A was 93.3％,which of group B was 90.0％,the difference was not statistically significant (x2 =0.218,P ＞ 0.05).The swelling subsided time [(24.2 ± 5.0) d vs.(22.9 ± 4.1) d],fracture healing time [(42.4 ± 9.5) d vs.(41.3 ± 9.4) d],postoperative recovery time of walking [(46.6 ± 9.2) d vs.(45.7 ± 9.0) d],AOFAS score [(87.3 ± 2.6) vs.(88.4 ±2.2)] between the two groups had no statistically significant differences(t =1.101,0.451,0.383,1.769,all P ＞0.05).The group A had 1 case in infection,the incidence rate of complications was 3.3％.The group B had no complication.The difference was not statistically significant in the incidence of complications between the two groups (x2 =1.017,P ＞ 0.05).Conclusion In the operative treatment for patients with grade Ⅳ pronation-external rotation ankle injury,whether choose ordinary steel plate fixation,or choose the locking plate fixation,the clinical effects of the two methods are both good,and the postoperative healing is fast.
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@#Objective To investigate the effects of morroniside on activation of caspase-3 in rats with focal cerebral ischemia/reperfusion. MethodsThe model was induced with occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. Vitamin E used for the positive control. The activity of caspase-3 was detected with spectrophotometry. ResultsCompared with sham group, the caspase-3 activity increased obviously in model rat. Compared with model group, Morroniside (30 mg/kg,90 mg/kg,270 mg/kg) decreased the activation of caspase-3 remarkably, which was dose-dependent (P<0.05). ConclusionMorroniside may reduce apoptosis by decreasing the activation of caspase-3 in rats.
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@#ObjectiveTo investigate the effects of morroniside on IL-1β in rat cortex with focal cerebral ischemia/reperfusion.MethodsThe animal model was induced by occlusion of middle cerebral artery with suture embolus, ischemia for 30 min, and reperfusion for 72 h in rats. The content of IL-1β was detected with enzyme linked immunosorbent assay(ELISA).ResultsCompared with sham group, the content of IL-1β increased obviously in model rat. Compared with model group, morroniside(30 mg/kg, 90 mg/kg, 270 mg/kg) and colchicine (0.06 mg/kg) decreased the content of IL-1β significantly (P<0.001).ConclusionMorroniside may protect the cortex from inflammatory factor IL-1β.
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@#Cerebral ischemia-reperfusion results in damage on neuron, leading to genes and proteins related to apoptosis activation. At the same time, generous cytokines released after cerebral ischemia-reperfusion can induce the apoptosis of the neuron. Many current studies have showed that the major damage mechanisms on apoptosis of the neuron are mitochondrion impairment, calcium overload, increased levels of oxygen radicals and so on. The advance research on the mechanism contributes to explore new neuroprotective drugs, and further identify the target and therapeutic effect of drug treatment.
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Aim The goal is to make a further comprehension of the pathogenesis of Parkinsons disease (PD) and hence add further knowledge for PD diagnosis by observing the functions of nitric oxide (NO) in striatum (STR). Method Microelectrodes were used to observe the effects of sodium nitroprusside (SNP), L-glutamic acid (GLU) and ?-aminobutyric acid (GABA) on STR neurons' spontaneous firing rates, and at the same time, we observed the effects of NO on GLU and GABA. Results 77% (51/66) of the tested neurons were excited by SNP, NO synthase inhibitor L-NAME antagonized the excitatory effect of SNP. Duringthe periods of microelectrophoresis GLU and GABA, SNP amplified the excitatory effect of GLU and weakened the inhibitory effect of GABA. L-NAME antagonized the excitatory effect of GLU.Conclusion These results demonstrated clearly that NO, GLU and GABA functions might converge in the same STR neurons. NO and GLU functions are excitatory whereas GABA function is inhibitory on the firing activities in STR neurons.