Expression and clinical significance of cyclin H and CDK7 in human hemangiomas / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the expression and clinical significance of cyclin H and cyclin-dependent kinase 7 (CDK7) in human hemangiomas.</p><p><b>METHODS</b>Immunohistochemistry technique was used to measure the expression of cyclin H and CDK7 proteins in proliferative, involuting hemangiomas and normal skin tissues. Immunohistochemical technique for factor VIII-related antigen was used to prove that the cells which expressed cyclin H and CDK7 were endothelial cells. Average optical density and positive area of the expression of cyclin H and CDK7 proteins in proliferative, involuting hemangiomas and normal skin tissues were measured by image analysis (HPIAS-1000).</p><p><b>RESULTS</b>The expression of cyclin H and CDK7 protein in proliferating hemangiomas was significantly higher than that in involuting hemangiomas and normal skin tissues (P < 0.01). But no significant difference was found in the expression of cyclin H and CDK7 protein between involuting hemangiomas and normal skin tissues (P > 0.05).</p><p><b>CONCLUSIONS</b>cyclin H and CDK7 may play an important role in the generation and development of human hemangiomas.</p>

The expression of p73 and c-fos protein in hemangioma and its significance / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the expression of p73 and c-fos protein and its significance in the development of children hemangioma.</p><p><b>METHODS</b>The quantitative expressions of p73 and c-fos protein in hemangioma and normal skin were detected by immunohistochemistry.</p><p><b>RESULTS</b>The expressions of p73 and c-fos protein were strong in proliferative hemangioma while they were very weak in involutional hemangioma and normal skin. There were significant differences between the proliferative and involutional hemangioma or the normal skin in the expressions of p73 and c-fos (P < 0.01). No statistical significances of p73 or c-fos P73 expressions were observed between involutional hemangioma and normal skin (P > 0.05).</p><p><b>CONCLUSIONS</b>P73 and c-fos may play an important role in the development and involution of skin hemangioma.</p>

Investigation of the influence of nitric oxide on the survival of a random pattern skin flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the changes of nitric oxide concentration in the distal portion of a random pattern skin flap and the influence of the exogenous L-arginine on the survival of the random pattern skin flap.</p><p><b>METHODS</b>A random pattern skin flap (7 cm x 2 cm) was cranially designed and elevated on the back of a Wistar rat. An image analysis technology was used to evaluate the survival rate of the skin flap, while a biochemistry method was used to test the concentrations of the NO in the tissue.</p><p><b>RESULTS</b>The survival area of the flap in the L-arginine-treated group was significantly enlarged (63.83 +/- 5.13)% (P < 0.01) in seven days postoperatively, compared with the control group (43.26 +/- 2.86)%. The NO concentration in the tissue was no statistic difference between all of the groups immediately after the operation (P > 0.05). But, the NO concentration in the control was decreasing at the beginning and then increasing slightly to reach the high level in 12 hours after the operation. It was thereafter slumped down to the baseline in 72 hours after the surgery. Although the changes in the L-arginine-treated group were quite similar to the control excepting of the extent, the NO concentration was kept in a higher level in the sequential time after the operation (P < 0.01, P < 0.01, P < 0.05 and P < 0.01).</p><p><b>CONCLUSIONS</b>The NO concentration in skin flap tissue after the elevation was going up slightly for a short time. The exogenous L-arginine could promote the NO concentration in the random pattern skin flap to protect it from ischemic injury.</p>