Effect of sterigmatocystin on histopathological changes in rats kidney and spleen

This study investigated the histopathological changes of kidney and spleen tissues in male rats intoxicated with sterigmatocystin. 48 mature albino rats were divided into 6 equal groups. The 1st and 2nd group were administered sterigmatocystin orally in doses of 1.3 and 2.6 mg/60 g BW for 10 days. The animals of the 4th and 5th groups received sterigmatocystin by intraperitoneal in doses of 0.25 and 0.5 mg/60 g BW for 10 days. The 3rd and 6th groups were control groups. Microscopic examination of the oral group revealed cortical hemorrhage, hyaline cast in some of renal tubules and necrosis of the epithelial cells lining many of these tubules in the kidney. These lesions were more prominent in rats treated intraperitoneally and with the increase of the dose of the toxin, while spleen sections of rats showed congestion and hemorrhage. The higher doses of mycotoxin caused atrophy and necrosis of lymphocytes

Formation of mycotoxins by toxigenic fungi isolated from green coffee bean in Egypt

Isolates [991] of toxigenic fungi belonging to the genera Aspergillus and Penicillium were isolated from green coffee bean in Egypt and were tested for their ability to produce mycotoxinsFungi were identified and relative frequency of their occurrence and percentage of seeds infection was also studied Ochratoxins were produced by 8% of A ochraceus; whereas, six out of 109 isolates of PViridicatum and one isolate of P Cyclopium Aflatoxin B1 were produced by seven isolates of AFlavus [4.9%] Six out of 51 isolates of A versicolor formed sterigmatocystin Isolates of A niger, P frequentans and P. citrinum did not produce mycotoxins

Rapid confirmatory test for aflatoxins using some microorganisms

Four bacterial cultures were studied in a search for an organism sensitive to aflatoxins [B1, B2, G1, G2 and M1] to be used as confirmatory bioassay for these mycotoxins. It was found that bacillus megaterium GAIM 1057 was sensitive to Afl. B1 and B2 but not sensitive to Afl. G1, G2 and M1, while bacillus cereus was found to be sensitive to Afl. B1, B2, G1 and G2; its sensitivity was found to be more pronounced B2 and G2. Staphylococcus aureus and Escherichia coli showed intermediate sensitivity to Afl. B2, G1 and G2 but was not sensitive to Afl. B1. All the test microorganisms did not give a response with Afl. M1. The assay is rapid [15-17 hr], simple and inexpensive and can be used to verify the presence in the samples of aflatoxins B1, B2, G1 and G2