RESUMO
Background/Aims: Differential diagnosis between aggressive osteoblastoma and low grade osteosarcoma may be very diffi cult or even impossible on a small biopsy. This study was designed to assess the usefulness of immunoexpression of COX-2 and osteocalcin in the differential diagnosis of the two tumour types. Methods: Immunostaining of COX 2 and osteocalcin were studied in 9 osteoblastomas and 30 osteosarcomas. Results: All osteoblastomas and 11/20 (55%) high-grade osteosarcomas showed COX-2 immunoreactivity. All low grade osteosarcomas were COX-2 negative. COX-2 was signifi cantly higher (p<0.002) in osteoblastomas 9/9 (100%) than in osteosarcomas 13/30 (43%) and in aggressive osteoblastomas versus low grade osteosarcomas (p<0.01). Osteocalcin was found in tumour cells of all osteosarcomas and osteoblastomas and in the osteoid matrix of 84% of osteosarcomas and 78% of osteoblastomas. Strong osteocalcin was signifi cantly higher (p<0.02) in osteoblastomas (78%) than in osteosarcomas (27%). Conclusion: COX-2 is a valuable marker in distinction between osteosarcoma and osteoblastoma. Negative COX-2 could confi rm the diagnosis of low grade osteosarcoma versus aggressive osteoblastoma. Intensity and distribution of osteocalcin may indicate the degree of osteoblastic differentiation.
RESUMO
Cysts of the larynx are rare lesions, most frequently located on the supraglottic region. This study included eight cases with supraglottic cyst. Five cases were males with an age average of 32 year old [22-60 years]. Two cases were females with an age average of 28 years [20, 36 years respectively]. One case was a boy, 8 years old. The cases were classified into four groups: three cases with aryepiglottic cyst [37.5%, two cases on the left side and one case on the right side], three cases with epiglottic cyst on the pharyngeal surface [37.5%, two on the left side and one case on the right side], one case with ventricular cyst [12.5%] and one case with vallecular cyst [12.5%]. Presentation, histopathological evaluations, acoustic and aerodynamic presentation were discussed