Flow cytometric analysis of peripheral T lymphocyte subpopulations in psoriasis

Psoriasis is a common inflammatory skin disorder, has received attention as a target for new pathogenesis and oriented therapies. Autoimmunity and T lymphocyte subsets are suggested to be involved in the development of psoriasis. The aim of this work is to asses the role of T lymphocyte subsets in the pathogenesis of psoriasis. We investigated the peripheral blood lymphocyte subpopulations obtained from psoriatic patient before and after treatment and from healthy controls, using two colour flow cytometry. We found highly significant suppression of total CD[3+] T Cells and CD[3+] CD[56+] NKT lymphocytes in psoriatic patient as compared to control. Also, we observed significant reduction of T helper cells in patients as compared to control. The highly significant reduction of CD[3+] T cell and CD[3+] CD[56+] NKT lymphocytes proved their actively involvement in the development of psoriasis

Increased production of vascular endothelial growth factor and some other cytokines in serum and ascitic fluid of cirrhotic patients with spontaneous bacterial peritonitis

The aim of this study was to determine serum and ascetic fluid vascular endothelial growth factor [VEGF], tumor necrosis factor- alpha [TNF-alpha], interleukin-6 [IL-6] and endotoxin, in cirrhotic patients and to search for a relationship between them and SBP. This study was performed on 40 cirrhotic patients with SBP, their ages ranged from 35 to 60 years, with mean of 47.5 +/- 11.6 years, 40 cirrhotic patients with non-infected ascites their ages ranged from 22 to 50 years, with mean of 34.5 +/- 6.5 years and 20 healthy control subjects with matched age and sex. From the results obtained it was concluded that VEGF, IL-6, TNF-alpha and endotoxin in the serum and ascetic fluid may become useful markers for diagnosis of SBP and for monitoring the treatment of cirrhotic patients