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1.
Int. j. morphol ; 41(1): 225-230, feb. 2023. tab
Artigo em Inglês | LILACS | ID: biblio-1430514

RESUMO

SUMMARY: The present research corresponds to a cross-sectional descriptive study in the anthropometric field, which allows coaches to develop standards to identify talent and optimize training in the discipline of beach volleyball. Its objective was to define the anthropometric profile and body composition of the Mexican Olympic beach volleyball teams in both sexes. The participants were couples one and two of Mexico in both sexes (defined by FIVB ranking), 4 women and 4 men with average age of 30.25±6.85 and 27.25±7.36 years. The technique used was Heath-Carter´s somatotype method and the anthropometric profile restricted ISAK protocol. Among the findings resulting from the research, unification can be observed in the male morphological characteristics (triceps skinfold, front thigh skinfold, calf skinfold, circumference of relaxed and contracted arm, waist, hip, femur diameter and BMI), as for the somatotype, both sexes of Mexican couples are positioned in mesomorphs-balanced. In relation to other studies consulted, balance in somatotype and muscular development is observed with high performance teams. Weight and height, as well as age, are important variables for the selection of talent and future optimal performance in world and professional volleyball.


La presente investigación corresponde a un estudio descriptivo transversal en el campo antropométrico, que permite a los entrenadores desarrollar estándares para identificar talentos y optimizar el entrenamiento en la disciplina de voleibol de playa. Su objetivo fue definir el perfil antropométrico y la composición corporal de las selecciones olímpicas mexicanas de voleibol de playa en ambos géneros. Los participantes fueron las parejas uno y dos de México en ambos géneros (definidos por ranking Fédération Internationale de Volleyball), 4 mujeres y 4 hombres con edad promedio de 30.25±6.85 y 27.25±7.36 años. La técnica utilizada fue el método del somatotipo de Heath-Carter y el protoolo ISAK del perfil antropométrico restringido. Entre los hallazgos resultantes de la investigación, se puede observar una unificación en las carácteristicas morfológicas masculinas (pliegue cuatáneotricipital, pliegue cutáneo anterior del muslo, pliegue citáneo de la pantorrila, circunferencia del brazo relajado y contraído, cintura, cadera, diámetro del fémur e IMC), en cuanto al somatotipo, ambos sexos de las parejas mexicanas se posicionan en mesomorfos-equilibrados. En relación a otros estudios consultados, se observa equilibrio en somatotipo y desarrollo muscular con equipos de alto rendimiento. El peso y la altura, así como la edad, son variables importantes para la selección del talento y en el futuro desempeño óptimo en el voleibol mundial y profesional.


Assuntos
Humanos , Masculino , Feminino , Adulto , Composição Corporal , Antropometria , Voleibol , Somatotipos , Estudos Transversais , México
2.
Int. j. morphol ; 39(1): 90-94, feb. 2021. tab
Artigo em Espanhol | LILACS | ID: biblio-1385328

RESUMO

RESUMEN: Determinar el perfil antropométrico y de composición corporal de los atletas ha sido -hasta hace poco- una herramienta fundamental para predecir el rendimiento deportivo y comportamiento biomecánico. Para este estudio, se evaluaron a 15 jugadores de la Selección Mexicana de Voleibol Varonil, con edad de 26,4 ± 4,73, previo a la XIV Copa Panamericana de Voleibol 2019. Se utilizó el protocolo ISAK para la toma antropométrica así como las ecuaciones de Carter & Heath para determinar el perfil antropométrico y somatotipo, el porcentajey kg de grasa, se calculó a partir de la ecuación de Durnin & Womersley, la masa muscular con el método De Rose y Gimares, y tejido óseo con la ecuación de Rocha. Resultados: el peso promedio fue de 85,45 kg (± 8,39), estatura 192,71 cm (± 7,15); somatotipo: endomorfo (2,3 ± 0,8), mesomorfo (4,0±1,4) y ectomorfo (3,6 ± 1,5). El % GC promedio fue de (3,6 ± 1,5), kg GC (11,2 ± 4,0), de % MM (43,8 ± 2,3), kg MM (37,3 ± 2,7), % TO (19,3 ± 1,8), KgTO (16,4 ± 1,2). Se concluye que la Selección Mexicana Varonil Mayor de Voleibol se encuentra dentro de los rangos competitivos de una Selección mayor; así como también muestra valores de referencia internacional. Presenta dos perfiles somatotípicos, endo-mesomórfico para jugadores defensivos y ecto-mesomórficos en jugadores ofensivos, beneficiando técnicamente su desempeño.


SUMMARY: Until recently, determining the anthropometric and body composition profile of athletes has been a fundamental tool for predicting sports performance and biomechanical behavior. For this study, 15 players form the Mexican Men`s Volleyball Team, aged 26.4±4.73, were evaluated prior to the beginning of the XIV 2019 Pan- American Volleyball Cup, using the ISAK protocol. As well as the Carter & Heath equation to determine the anthropometric profile and somatotype, the percentage and Kg of fat, with the Durnin & Womersley equation, the De Rose and Gimares muscle mass, and bone tissue with the Rocha equation. The average weight was 85.45 kg (± 8.39), height 192.71 cm (±7.15); somatotype: endomorphic (2.3 ± 0.8), mesomorphic (4.0 ± 1.4), and ectomorphic (3.6 ± 1.5). The overage % GC was (3.6 ± 1.5). kg GC (11.2 ± 4.0), de % MM (43.8 ± 2.3) kg MM (37.3 ± 2.7), % TO (19.3 ± 1.8), kg TO (16.4 ± 1.2). It is concluded that the Mexican Men`s Volleyball Team is within the competitive ranges of a larger international teams, in addition, and as international reference values, the Selection presents two somatotypes profile, endo-mesomorphic for defensive players and ecto-mesomorphic for offensive players, technically benefiting their performance.


Assuntos
Humanos , Masculino , Adulto , Composição Corporal , Antropometria , Voleibol , Somatotipos , Estudos Transversais , México
3.
Rev. cuba. med ; 60(supl.1): e2505, 2021. graf
Artigo em Espanhol | CUMED, LILACS | ID: biblio-1408966

RESUMO

Introducción: Los quistes enterogénicos son malformaciones producidas durante el período de diferenciación y desarrollo embriológico del intestino primitivo. Objetivo: Presentar una malformación digestiva infrecuente en la edad adulta. Caso clínico: Paciente de 58 años que acude por dolor abdominal y aumento de volumen en hipogastrio. Al examen físico se palpa masa en hipogastrio de 15 cm de diámetro. La tomografía axial computarizada simple y contrastada mostró una masa tumoral en la excavación pélvica hipodensa con densidad de 18 UH, pared gruesa, de 2 cm, que realza con el contraste y agenesia renal derecha. El estudio anatomo-patológico informa quiste enterogénico. Discusión: La clínica de las duplicaciones intestinales es inespecífica, el dolor abdominal recurrente y la presencia de masa abdominal, son frecuentes. El diagnóstico preoperatorio de las duplicaciones intestinales es infrecuente, depende de la disponibilidad de estudios de imagen y la sospecha clínica. Es infrecuente la asociación de quiste enterogénico y agenesia renal. Conclusión: Se presenta una paciente masculino con una masa pélvica de etiología dudosa y agenesia renal derecha, en el que se diagnosticó un quiste enterogénico. La asociación de quiste enterogénico y agenesia renal es una malformación rara en el adulto(AU)


Introduction: Enterogenic cysts are malformations produced during the period of differentiation and embryological development of the primitive intestine. Objective: To report an rare digestive malformation in adulthood. Clinical case report: A 58-year-old patient came for abdominal pain and increased volume in the hypogastrium. On physical examination, a 15-cm diameter mass was palpated in the hypogastrium. Simple and contrast computerized axial tomography showed a tumor mass in the hypodense pelvic excavation with a density of 18 HU, a thick wall of 2 cm, which was enhanced with contrast and right renal agenesis. The anatomo-pathological study reports an enterogenic cyst. Discussion: The symptoms of intestinal duplications are nonspecific, recurrent abdominal pain and the presence of an abdominal mass are frequent. The preoperative diagnosis of intestinal duplications is occasional, it depends on the availability of imaging studies and clinical suspicion. The association of enterogenic cyst and renal agenesis is uncommon. Conclusion: A male patient is reported, with a pelvic mass of doubtful etiology and right renal agenesis, in whom an enterogenic cyst was diagnosed. The association of enterogenic cyst and renal agenesis is a rare malformation in adults(AU)


Assuntos
Humanos , Feminino , Cistos Ovarianos/diagnóstico por imagem , Tomografia Computadorizada de Emissão de Fóton Único/métodos , Rim Único/epidemiologia
4.
Rev. cuba. med ; 57(2)abr.-jun. 2018. ilus
Artigo em Espanhol | LILACS, CUMED | ID: biblio-985557

RESUMO

El término amiloidosis cardiaca hace referencia a la afección del corazón como consecuencia del depósito de amiloide en el tejido cardiaco, ya sea en el contexto de una afección sistémica o de una forma localizada. Se presenta un caso donde se analiza la fisiopatología y el diagnóstico de esta enfermedad. La miocardiopatía por amiloidosis es una enfermedad inflamatoria infiltrativa que con frecuencia no es sospechada. El ecocardiograma no siempre permite identificar el clásico patrón infiltrativo restrictivo(AU)


The term cardiac amyloidosis refers to a heart affection which is a consequence of the accumulation of amyloid in the heart tissue that can appear in the context of a systemic affection or in a localized form. A case is presented and it is analyzed the physiopathology and the diagnosis of this disease. Miocardiopathy by amyloidosis is an infiltrative inflammatory disease that is not frequently suspected. Echocardiogram not always allows identifying the classic restrictive infiltrative pattern(AU)


Assuntos
Humanos , Insuficiência Cardíaca , Amiloidose/etiologia , Cardiomiopatias
5.
Salud UNINORTE ; 33(2): 252-258, mayo-ago. 2017.
Artigo em Espanhol | LILACS | ID: biblio-903646

RESUMO

Resumen El propósito de este documento fue reportar los beneficios de la actividad física, entrenamiento intervalo y entrenamiento continuo moderado en adultos sedentarios y físicamente activos. La actividad física involucra cualquier movimiento corporal que produce un aumento en el gasto energético en el metabolismo, mientras que el entrenamiento intervalo y entrenamiento continuo moderado puede ser utilizado para controlar el programa de cargas de entrenamiento (intensidad, volumen y pausa). Los beneficios que se han reportado cuando se realiza actividad física son: el incremento o mantenimiento de la condición física muscular, funciones cognitivas, cardiorespiratoria, equilibrio, peso corporal, control de la obesidad; todos ellos disminuyen los riesgos de enfermedades cardiovasculares, enfernedades crónicorrespiratorias, diabetes, presión alterial alta, sindrome metabolico, cáncer de colon, depresión y todas las causas de mortalidad. En contraste, la falta de actividad fisica ha ha sido identificada como factor de riesgo y está asociada a diversas enfemedades no transmisibles a nivel mundial. En este documento puntualizamos dos tipos de entrenamiento que han tenido aplicaciones para la salud en adultos. Este trabajo podría ayudar a promover la salud calidad de vida de la población adulta y eliminar el sedentarismo mediante la prescripción de la actividad física para la salud.


Abstract The purpose of this paper was report the benefit of the physical activity, interval training and moderate continuous training in sedentary and physically active adults. The physycal activity involve whatever bodily movement that produce a increased in the metabolic energetic expenditure. While that the interval training and moderate continuous training can be used for the training load schedule (intensity, volume and rest). While that the interval training and moderate continuous training can be used for the load training charge (intensity, volume and rest). The benefit that have been reported is the increasing or maintaining muscle fitness, cognitive and cardio- respiratory function, balance, body weight, obesity control, all reduce the risk of cardiovascular disease, cáncer, chronic respiratory diseases, diabetes, high blood pressure, metabolic syndrome, colon cancer, depression, and all causes of mortality. In contrast, the lack of physycal activity had been identified as risk factor and associated with various non comunicable diseases worldwide. In this paper, we point two types of training that had aplications for health in adults. This document could help to promote health, quality of live of the adult population and eliminate the physical inactivity by prescripcion of physical activity for the health.

6.
Int. j. morphol ; 33(2): 566-570, jun. 2015.
Artigo em Espanhol | LILACS | ID: lil-755511

RESUMO

La patología biliar litiásica es frecuente en nuestro país, con prevalencias entre 30% y 50%; y la ictericia obstructiva secundaria a coledocolitiasis (IOC), constituye un motivo de consulta frecuente en los servicios de urgencia. Por otra parte, la papilotomía endoscópica (PE) post colangiografía retrógrada endoscópica (CRE), constituye el tratamiento de elección en estos casos; sin embargo, es un procedimiento no exento de complicaciones. El objetivo de este estudio, es describir la MPO e identificar posibles factores de riesgo (FR) asociados a MPO, en pacientes con IOC, sometidos a PE. Serie de casos retrospectiva, de pacientes con IOC, a quienes se les realizó CRE y ulterior PE. La variable resultado fue desarrollo de MPO (hemorragia, perforación y pancreatitis). Otras variables de interés fueron canulación, dificultad de ésta, desarrollo de PE, uso de pre corte y mortalidad. La recolección de datos se realizó mediante una pauta ad-hoc, en la que se registraron las variables extraídas desde el protocolo operatorio y la ficha clínica. Se aplicó estadística descriptiva y analítica (Chi2 de Pearson y exacto de Fisher) para estimar fuerza de asociación. Se intervinieron 200 pacientes. La Media de edad fue de 60±18 años; 62% eran mujeres (n= 124). Se registró MPO en 32 casos (16,0%): Perforación (0,5%), pancreatitis (2,0%) y hemorragia (13,5%). La serie no registró mortalidad. No se logró objetivar asociación entre la variable "canulación difícil" y las variables hemorragia (p= 0,214); pancreatitis (p= 0,519); ni perforación (p= 1). Sin embargo, se verificó asociación entre el desarrollo de hemorragia y la realización de PE (p= 0,017). La hemorragia es la MPO más frecuente en esta serie; y la PE es un FR para el desarrollo de hemorragia.


Bileduct stones is prevalent in our country, with prevalences between 30% and 50%; and obstructive jaundice secondary to choledocholithiasis (OJC), is a frequent reason of consultation in emergency services. Furthermore, endoscopic papillotomy (EP) post ERCP is the treatment of choice in these cases; however, it is not free of complications (POM). The aim of this study is to describe POM and identify potential risk factors (RF) associated with POM in patients with OJC, underwent PE. Retrospective case series of patients with OJC, who underwent ERCP and subsequent PE. Outcome variable was the development of POM (bleeding, perforation, and pancreatitis). Other variables of interest were cannulation, difficulty of this, developing PE, using precut and mortality. Data collection was performed by an ad-hoc pattern in which the variables extracted from surgical protocols and clinical data were recorded. Descriptive and analytical statistics (Pearson Chi2 and Fisher's exact test) were applied to assess strength of association. 200 patients were operated. The mean age was 60±18 years; 62% were women (n = 124). MPO was recorded in 32 cases (16.0%): perforation (0.5%), pancreatitis (2.0%) and bleeding (13.5%). The series does not record mortality. It was not possible to objectify association between "difficult cannulation" and the variables bleeding (p= 0.214); pancreatitis (p= 0.519); and perforation (p= 1). However, association between bleeding and performing PE (p= 0.017) was observed. Hemorrhage is the most common cause of MPO in this series; and PE is a RF for the development of bleeding.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Colangiopancreatografia Retrógrada Endoscópica/efeitos adversos , Colangiopancreatografia Retrógrada Endoscópica/estatística & dados numéricos , Coledocolitíase/cirurgia , Icterícia Obstrutiva/cirurgia , Coledocolitíase/complicações , Seguimentos , Hemorragia/etiologia , Icterícia Obstrutiva/etiologia , Pancreatite/etiologia , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Esfinterotomia Endoscópica
7.
Rev. colomb. psiquiatr ; 37(3): 385-396, sep. 2008. tab
Artigo em Espanhol | LILACS | ID: lil-636425

RESUMO

Objetivos: Determinar la prevalencia de psicopatología en los candidatos a cirugía bariátrica en la Clínica Reina Sofía, entre octubre y noviembre de 2007, e identificar algunos potenciales factores de riesgo para su aparición. Método: Se realizó un estudio descriptivo con corte transversal comparativo, en el cual participaron 50 candidatos a cirugía bariátrica, a quienes después de firmar el consentimiento informado se les aplicó la Structured Clinical Interview (SCID), para el DSM-IV, y preguntas estructuradas para la exploración sistemática de las variables estudiadas. Resultados: La muestra de 50 pacientes se conformó en un 62% (n=31) por mujeres. La edad promedio de los participantes fue de 45,2 (DE=12,94) y el promedio en el índice de masa corporal (IMC) fue de 38,92 Kg/m² (DE=3,53). La prevalencia de psicopatología fue del 38% (n=19), clasificada como trastornos del estado del ánimo: 24% (n=12); trastornos adaptativos: 8% (n=4), y trastornos de ansiedad: 6% (n=3). Al comparar los sujetos de acuerdo con la presencia de psicopatología, se encontró una asociación estadísticamente significativa entre el antecedente de abuso sexual infantil y la presencia de psicopatología (OR=10,71; IC 95%: 1,14-100,52). No hubo relación con variables sociodemográficas, grado de obesidad o problemas psicosociales. Conclusiones: El estudio mostró una asociación estadísticamente significativa entre la presencia de psicopatología y el antecedente de abuso sexual infantil...


Objectives: To determine the prevalence of psychopathology among bariatric surgery candidates at the Reina Sofía Clinic during October-November 2007, and to identify some factors associated to psychopathology in this population. Method: This is a descriptive study with a comparative transversal analysis. 50 bariatric surgery candidates were considered into study, who accepted to participate in the study and signed written informed consent. These patients were then assessed with Structured Clinical Interview (SCID), for DSM-IV, and structured questions for the systematic variables exploration. Results: In the study group (n=50), 62% (n=31) were women, the mean age was 45.2 years (SD=12.94) and the mean body mass index (BMI) of the group was 38.92 Kg/m² (SD=3.53). The psychopathology prevalence was 38% (n=19), with mood disorders: 24% (n=12); adaptative disorders; 8% (n=4), and anxiety disorders: 6% (n=3). The comparative analysis by the psychopathology presence showed a signifi cant correlation with history of sexual abuse and psychopathology (OR=10.71; 95% C.I.: 1.14-100.52). The analysis did not show significant relation among demographic characteristics, obesity grade or social problems and the risk of psychopathology. Conclusions: the study shows a significant correlation between child sexual abuse history and psychopathology...


Assuntos
Obesidade , Psicopatologia , Abuso Sexual na Infância
8.
Rev. salud pública ; 10(3): 374-385, jul. 2008. tab
Artigo em Espanhol | LILACS | ID: lil-497301

RESUMO

Objetivos Caracterizar pacientes con intento suicida atendidos en Psiquiatría en la Unidad Primaria de Atención (UPA) La Estrella entre octubre-2004 y octubre-2005 y determinar la persistencia de ideación suicida, los factores asociados así como factores predictores. Métodos Caracterización inicial de pacientes con intento suicida (n=156). Diseño posterior de casos y controles (n=77) según persistencia de ideación suicida (n=27). Se determinaron los factores asociados y la predicción del riesgo suicida con un modelo multivariado. Resultados Los casos aumentaron de 6 en octubre-2004 hasta 10 en octubre-2005, con promedio mensual: 12 ± 3,6 casos. El 80 por ciento tenía 30 años o menor edad, 66,7 por ciento eran mujeres, 24,4 por ciento estudiantes y 20,5 por ciento desempleados. Como desencadenantes: disfunción familiar y conflictos de pareja en 35,2 por ciento cada uno. El 49 por ciento consumió alcohol previo al intento suicida. La persistencia de ideación suicida se asoció con tener 31 años o más, desempleo, más de un intento suicida previo, conflictos sin resolver y disfunción familiar. La no resolución de conflictos, OR=106,7 (IC 95 por ciento: 6,8, 1668,4) y la disfunción familiar, OR=11,05 (IC95 por ciento: 1,7, 71,2) fueron predictores de la persistencia de ideación suicida en el modelo de regresión logística. Conclusión La no resolución de conflictos y la disfunción familiar, predictores de la persistencia de ideación suicida en sujetos con intento suicida, el consumo de alcohol y otros factores asociados podrían controlarse con programas de prevención y promoción de salud mental.


Objective Characterising patients attempting suicide who were evaluated by psychiatry at the Primary Care Unit (PCU) " La Estrella " from October/2004 to October/2005 and determining persistent suicidal ideation and associated factors. Methods An initial description of attempted suicide patients (n=156). A subsequent case control study (n=77) determined persistent suicidal ideation cases (n=27). A multivariate model was used for determining the remaining factors associated with persistent suicide ideation. Results Cases increased from 6 in October/2004 to 10 in October/2005, average 12 ± 3,6 cases monthly. 80 percent were aged 30 or younger, 66,7 percent were female, 24,4 percent were students and 20,5 percent were unemployed. 35,2 percent suffered family dysfunction and 35.2 percent had conflicts between couples as precipitant factors. 49 percent had consumed alcohol prior to the suicide attempt. Persistent suicidal ideation was associated with being 31 years old or older, being unemployed and having a higher number of previous suicide attempts, unresolved conflicts and family dysfunction. Logistical regression revealed unresolved conflicts (OR=106.7; 6,8; 1 668,4 95 percent CI) and family dysfunction (OR=11.05; 1,7; 71,2 95 percent CI) as predictor variables for persistent suicidal ideation. Conclusion Unresolved conflicts and family dysfunction were found to be predictor factors for persistent suicidal ideation in subjects who had attempted suicide. Consuming alcohol and other associated factors should be controlled by mental health prevention and promotion programmes.


Assuntos
Adulto , Feminino , Humanos , Masculino , Imaginação , Tentativa de Suicídio/psicologia , Colômbia , Unidades Hospitalares , Atenção Primária à Saúde
9.
Arch. med. res ; 25(2): 241-5, 1994. tab
Artigo em Inglês | LILACS | ID: lil-198813

RESUMO

Bovine babesiosis, caused by parasites of the genus babesia, is one of the world's most severe tick-borne problems of cattle in temperate to tropical areas. In the America Babesia bovis and B bigemina are the causative agents, with the former considered to produce the greatest economic impact. The great complexity of the relationship causal agent-vector-host has severely hindered the efforts towards the production of a safe, long-lasting, solid-protection inducing vaccine. Recent importan contributions that have encourage the study of these agents include the development of in vitro cultivation systems, procedures for the isolation of single infected-erythrocytes, density gradient-based centrifugation systems for the isolation and concentration of both infected erythrocytes and merozoites, isozyme detection and differentitation systems that help discrimate between parasite species, and development of DNA-based diagnostics and characterization protocols. Currently, the study of the cellular immune response against these parasites is taking new endeavors in order to discern the relationship between B cells, T cell, macrophages and their product and parasites leading to the establishment of solid, long-lasting protection. In an attempt to design a rational vaccine, T cell lines and clones are being established, and phagocytosis of infected erythrocytes and their antigens studied to try to pinpoint relevant epitopes


Assuntos
Animais , Bovinos , Anticorpos Monoclonais/imunologia , Babesiose/parasitologia , Doenças dos Bovinos/parasitologia , Bovinos/parasitologia , Parasitos/isolamento & purificação
10.
Mem. Inst. Oswaldo Cruz ; 87(supl.3): 207-11, 1992. tab, ilus
Artigo em Inglês | LILACS | ID: lil-121103

RESUMO

The development of a repetitive DNA probe for Babesia bigemina was reviewed. The original plasmid (p(Bbi)16) contained an insert of B. bigemina DNA of approximately 6.3 kb. This probe has been evaluated for specificityand analytical sensitivity by dot hybridization with isolates from Mexico, the Caribbean region and Kenya. A partial restriction map has been constructed and insert fragments have been subcloned and utilized as specific DNA probes. A comparison of 32P labelled and non-radioactive DNA probes was presented. Non-radioctive detection systems that have been used include digoxigenin dUTP incorporation, and detection by colorimetric substrate methods. Derivatives from the original DNA probe have been utilized to detect B. bigemina infection in a) experimentally inoculated cattle, b) field exposed cattle, c) infected Boophilus microplus ticks, and d) the development of a PCR amplification system


Assuntos
Babesiose/diagnóstico , DNA , Eucariotos
11.
Mem. Inst. Oswaldo Cruz ; 87(supl.3): 213-7, 1992. tab, ilus
Artigo em Inglês | LILACS | ID: lil-121104

RESUMO

An epidemiological survey was conducted in south east Mexico, in an effort to establish the serological reactivity and carrier status to Babesia bigemina of an indigenous cattle population. The prevalance was obtained through the Indirect Fluorescent Antibody Test (IFAT), using an in vitro culture-derived B. bigemina antigen. A specific, digoxigenin-coupled, ~6kb B. bigemina-DNA probe (BBDP), was used to indicate the presence of the parasite. Serum samples from 925 animals of all ages, were obtained within the three regions (I, II, III) of the state of Yucatan and tested by IFAT. In addition, whole blood samples draw from 136 of the same animals of region II were analyzed using the BBDP. Positive IFAT (IFAT+) reactions were observed in 531 sera for a 57% overall prevalence. Regional values were: I = 157 + (56%), II = 266 + (68%) and III = 108 + (42%). Only 32 (23%) of the blood samples tested with BBDP showed distinctive hybridization signal, in contrast with 100 (73%) IFAT + animals. The responses distribution for IFAT vs. BBDP was: +/+ 23, +/- 77, -/+ 9 and -/- 27 respectively. It was found that the analytical sinsitivity of BBDP appears to be low for its utilization is widespread epidemiological surveys. It was considered, however, that the colorimetric probe mifht to be useful to safely detect transmission prone carriers, since it is able to detect parasitemias as low as 0.001


Assuntos
Babesiose/epidemiologia , DNA , Fluorescência
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