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1.
Braz. j. microbiol ; 47(3): 665-674, July-Sept. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-788963

RESUMO

ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions (T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and pH = 6.6) for high AA synthesis (9.26 EU/mL). However, the maximum PA level (9.77 EU/mL) was obtained at pH 7.1 and 37.8 °C. Under these conditions, a considerably high reduction (between 69.9 and 77.8%) of the initial chemical oxygen demand of the waste was achieved. In verification experiments under the optimized conditions for production of each enzyme, the AA and PA obtained after 15 h of incubation were, respectively, 9.35 and 9.87 EU/mL. By using the Luedeking and Piret model, both enzymes were classified as growth-associated metabolites. Protease production delay seemed to be related to the consumption of non-protein and protein nitrogen. These results indicate that the brewery waste could be successfully used for a high scale production of amylases and proteases at a low cost.


Assuntos
Peptídeo Hidrolases/biossíntese , Bacillus subtilis/metabolismo , Fermentação , Amilases/biossíntese , Resíduos Industriais , Temperatura , Cinética , Biotransformação , Metabolismo dos Carboidratos , Concentração de Íons de Hidrogênio
2.
Rev. cuba. hig. epidemiol ; 29(1): 66-71, ene.-jun. 1991. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-102641

RESUMO

Se analizan los resultados de 3 849 muestras de queso estudiadas durante el período 1985-1988 en los Centros Provinciales de Higiene y Epidemiología. Los valores encontrados están entre 1 y 1,7 %. Se valoran los factores que pueden incidir en la contaminación de este alimento. Se proponen medidas de control


Assuntos
Queijo/análise , Contaminação de Alimentos , Salmonella/isolamento & purificação
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