RESUMO
OBJECTIVE@#To improve the correct rate of ABO genotyping by meliorating AS-PCR primer.@*METHODS@#The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed.@*RESULTS@#The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer.@*CONCLUSION@#The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.
Assuntos
Humanos , Sistema ABO de Grupos Sanguíneos/genética , Alelos , Sequência de Bases , Primers do DNA/genética , Genótipo , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Sensibilidade e EspecificidadeRESUMO
OBJECTIVE@#To identify sarcosaphagous flies and their larvae, pupa.@*METHODS@#Sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing.@*RESULTS@#The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples.@*CONCLUSION@#It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.
Assuntos
Animais , Humanos , Sequência de Bases , China , Primers do DNA , DNA Mitocondrial/genética , Dípteros/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Medicina Legal , Genes de Insetos , Larva/genética , Filogenia , Reação em Cadeia da Polimerase/métodos , Pupa/genética , Análise de Sequência de DNA/métodos , Especificidade da EspécieRESUMO
<p><b>OBJECTIVE</b>To study genetic polymorphism of 9 STR loci in Han nation population in Shangdong Province.</p><p><b>METHODS</b>We investigated 100 unrelated individuals of Han nation population in Shandong Province and studied their genetic distribution of 9 STR loci and Amelogenin locus. Primers of 10 loci were labeled with the NHS-ester fluorescent dye 5-FAM (blue), Joe (green) or NED (yellow). The data of Han nation population were generated by multiple amplification and subjected to GeneScan, genotype and genetic distribution analysis.</p><p><b>RESULTS</b>83 alleles and 220 genotypes were observed, with the corresponding frequency of 0.0050-0.4050 and 0.0100-0.2100. The average of heterozygosity was 0.7778, the accumulated discrimination power was 0.9999. The accumulated probability of exclusion paternity was 0.9999. The polymorphism information content was 0.5823-0.8396.</p><p><b>CONCLUSIONS</b>Chi-Square test indicated that the distribution of genotypes agreed with Hardy-Weinberg equilibrium.</p>